MicroRNA-27a promotes myoblast proliferation by targeting myostatin

MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression

“…Besides, we also observed an inverse correlation between expressions of myostatin mRNA and miR-27a in fat and heart of pigs and in proliferating porcine myoblasts. The reciprocal expression of miR-27a and myostatin has also been reported in other studies (Allen and Loh, 2011;Huang et al, 2012;Chen et al, 2014).…”

“…High expression of miR-1/ 206 in muscle inhibits transcript level of myostatin, which contributes to muscular hypertrophy (Clop et al, 2006). In our previous work, we demonstrated that miR-27a suppresses transcript level of mouse myostatin (Huang et al, 2012). It should be pointed out that mature miR-27a sequence (5'-UUCACAGUGGCUAAGUUCCGC-3') is well conserved among mammalian species.…”

“…MiR-27a, a member of miR-27 family, which has negative impacts on kinds of cells through different pathways, has been demonstrated to promote murine myoblast proliferation and differentiation through targeting myostatin in our previous studies (Huang et al, 2012;Chen et al, 2014). Nevertheless, we have no idea whether miR-27a could also promote porcine myoblast proliferation via downregulation of myostatin expression.…”

“…Luciferase reporter assay was performed as previously described (Huang et al, 2012). Briefly, the HEK 293T cells were plated into a 24-well plate and maintained in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Invitrogen) at 37°C, 5% CO 2 in a humidified atmosphere.…”

“…Proliferating porcine myoblasts were determined by using the Click-iT EdU Alexa Fluor 594 Imaging Kit (Invitrogen). EdU proliferation assay was performed as previously described (Huang et al, 2012), with minor modification. Briefly, porcine myoblasts were incubated with 10 μM EdU for 6 h before fixation, permeabilization, and EdU staining.…”