MicroRNA‐330 inhibits growth and migration of melanoma A375 cells: In vitro study

Abstract

Melanoma skin cancer is one of the main causes of male cancer‐related deaths worldwide. It has been suggested that miR‐330‐5p can act as a tumor suppressor in various types of cancers. So, in this study, we replaced miR‐330 in melanoma cancer cells by vector‐based miR‐330 to evaluate the effects of this microRNA on the growth and migration inhibition of melanoma cancer cells, and to determine the molecular mechanisms underlying its action. By using the MTT assay, the IC50 of Geneticin antibiotic was obtained as 460 µg/mL. The results of the qRT‐PCR showed the increased expression level of miR‐330 and decreased expression levels of MMP‐9, CXCR4, Vimentin, melanoma cell adhesion molecule, AKT1, and E2F1 messenger RNA in A375 transfected cells. The cytotoxicity assay results demonstrated the inhibition of cancer cells proliferation. Furthermore, the wound healing test results showed a migration reduction of transfected cells with miR‐330 compared with nontransfected ones. In addition, 4′,6‐diamidino‐2‐phenylindoleLB: Luria‐Bertani (DAPI) staining revealed the significant nucleus fragmentation in miR‐330 replaced cells, which correspond to apoptosis induction in replaced cells. The results showed that increase in miR‐330 expression level could significantly inhibit the tumor cell growth and the migration of melanoma cancer cells.

Exosomal MicroRNAs Contribute to Cognitive Impairment in Hypertensive Patients by Decreasing Frontal Cerebrovascular Reactivity

“…Previous studies indicated that the expression of miRNA-330 was significantly decreased in human prostate cancer, melanoma skin cancer, and colorectal cancer, which inversely correlated with its direct target specificity protein 1 (Sp1), E2F1 transcription factors, and thymidylate synthase (TYMS) expression. After overexpression of miRNA-330 in these cancer cells, cell growth, migration, and invasion capability were suppressed (Lee et al, 2009;Mao et al, 2013;Xu et al, 2017;Sehati et al, 2020). However, miRNA-330 overexpression promoted cellular proliferation of glioblastoma through directly targeting the 3 UTR of SH3GL2 gene (Qu et al, 2012).…”

miR-330 targeting BCO2 is involved in carotenoid metabolism to regulate skin pigmentation in rainbow trout (Oncorhynchus mykiss)

“…Although the biologic functions of most miR-NAs are not yet clarified, there is growing evidence that they perform essential roles in cell proliferation and apoptosis [7]. So far, several miRNAs, such as miR-330, miR-7013, and miR-18a, have been identified to regulate the apoptotic pathways of melanoma cells via targeting specific genes [40][41][42]. Moreover, overexpression of miR-330 can also suppress proliferation and induce apoptosis in other cell types [43,44], thus, miR-330 is often considered a pro-apoptotic factor.…”

Clinical Significance and Potential Regulatory Mechanisms of Serum Response Factor in 1118 Cases of Thyroid Cancer Based on Gene Chip and RNA-Sequencing Data

“…An unpublished miR-330-5p was selected to carry out preliminary dual-luciferase reporter assay, which confirmed that miR-330-5p can bind the 3'UTR of SRF. The expression level and mechanism of miR-330-5p have been studied in various cancers, including melanoma [37] hepatocellular carcinoma [38,39], pancreatic cancer [40,41], cervical cancer [42], prostate cancer [43], and non-small cell lung cancer [44]. However, the expression and function of miR-330-5p in TC and its molecular mechanism have not been clarified, and these topics warrant further study.…”