MicroRNA-331-3p Suppresses Cervical Cancer Cell Proliferation and E6/E7 Expression by Targeting NRP2

High-risk human papillomavirus (HPV) infection is the etiological agent of some malignancies such as cervical, oral and oropharyngeal cancers. Kaposi sarcoma-associated herpesvirus (KSHV) represents a principal causative agent of several human cancers arising in those immunocompromised patients. Interestingly, KSHV DNA has been detected in the oral cavity and the female genital tract, although its detection rate in cervical samples is very low and few reports are about KSHV/HPV co-infection. Therefore, it remains unclear about the role of KSHV co-infection in the development of HPV-related neoplasias. In the current study, we report that HPV16-integrated cervical cancer cell-line SiHa is susceptible to KSHV latent infection and replication. We also have found that KSHV infection or viral latent proteins are capable of reducing HPV16 E6/E7 expression through the manipulation of cellular microRNA function. Array analysis indicates that KSHV infection induces some inflammatory cytokines/chemokines production as well as up-regulates a series of interferon-induced genes expression, which may facilitate host immune defense system attacking these co-infected cells and clearance of viruses. Together, our data have provided possible explanations for very low detection rate of KSHV shedding as well as of KSHV/HPV co-infection in cervical samples and/or cervical cancer cells.

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“…One of potential mechanisms is through cellular microRNAs such as miRNA129-5p and miRNA331-3p [19, 20]. Zhang et al .…”

Section: Resultsmentioning

confidence: 99%

KSHV co-infection down-regulates HPV16 E6 and E7 from cervical cancer cells

et al. 2017

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“…Here, in our study, miR-331-3p was shown to be significantly down-regulated in EAC patients with recurrence compared with those without. It has been demonstrated that overexpression of miR-331-3p inhibited the proliferation and migration of various cancer cells, suggesting the role of miR-331-3p as a tumor suppressor 22 – 26 . Functional data provided evidence that miR-331-3p could downregulate the expression of human epidermal growth factor receptor 2 (HER2).…”

Section: Discussionmentioning

confidence: 99%

Serum miR-331-3p predicts tumor recurrence in esophageal adenocarcinoma

Zhang

Zheng

et al. 2018

Sci Rep

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MicroRNAs (miRNAs) may contribute to the initiation and progression of cancer. The role of circulating miRNAs as predictors of recurrence in esophageal adenocarcinoma (EAC) has not been extensively explored. Here we measured the expressions of 167 miRNAs in serum samples from a discovery cohort of 72 EAC patients (32 patients with recurrence and 40 patients without). A rank sum test was performed to identify differentially expressed miRNAs. Cox regression model was applied to estimate the effect of miRNA expression on recurrence-free survival. The eligible miRNAs were then validated in an independent cohort of 329 EAC patients (132 patients with recurrence and 197 patients without). miR-331-3p was identified and confirmed to be differentially expressed between EAC patients with and without recurrence and associated with recurrence-free survival. In both cohorts, the expression of miR-331-3p was consistently decreased in patients with recurrence compared to those without (P < 0.05). Using patients with low expression of miR-331-3p as reference, those with high expression had HRs for recurrence of 0.45 (95% CI, 0.21-0.96, P = 0.040) and 0.55 (95% CI, 0.38–0.78, P = 0.001) in the discovery and validation cohorts, respectively. Therefore, serum miR-331–3p may be a useful biomarker for identifying EAC patients at high risk of recurrence.

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“…miR-331 was reported to be dysregulated in various cancers. For example, miR-331 was found to be significantly upregulated in pancreatic cancer and cervical cancer [24,25]. Additionally, miR-331 was reported to be downregulated in colorectal cancer and ovarian cancer [26,27].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR-331 Indicates Poor Prognosis and Promotes Progression of Breast Cancer

et al. 2020

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Background: With the increasing number of cases of breast cancer every year, the exploration of novel biomarkers has drawn attention. miR-331 has been demonstrated to play a role in various cancers, but its role in breast cancer is still unknown. Methods: In this study, we included 121 patients with breast cancer treated at Affiliated Hospital of Weifang Medical University. Breast cancer tissues and adjacent normal tissues were collected during the surgery. The expression of miR-331 in breast cancer tissues and cell lines was detected by qRT-PCR assay. Then, with the help of Kaplan-Meier survival and Cox regression analyses, the role of miR-331 in the prognosis of breast cancer was analyzed. Finally, the effect of miR-331 on cell proliferation, migration, and invasion was investigated with CCK-8 assay and transwell assay. Results: miR-331 was significantly upregulated in breast cancer tissues compared with normal tissues. The overexpression of miR-331 was associated with lymph node metastasis, TNM stage, and poor prognosis. From the results of Cox regression analyses, it was found that miR-331 served as an independent indicator in the prognosis of breast cancer. In addition, miR-331 was also found to be upregulated in breast cancer cells, which promoted cell proliferation, migration, and invasion of breast cancer. Conclusion: As shown from our data, miR-331 may be a potential prognostic biomarker in breast cancer. Moreover, the development and progression of breast cancer may involve miR-331. These findings suggest a novel therapeutic target for the treatment of breast cancer.

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MicroRNA-331-3p Suppresses Cervical Cancer Cell Proliferation and E6/E7 Expression by Targeting NRP2

Cited by 49 publications

References 50 publications

KSHV co-infection down-regulates HPV16 E6 and E7 from cervical cancer cells

KSHV co-infection down-regulates HPV16 E6 and E7 from cervical cancer cells

Serum miR-331-3p predicts tumor recurrence in esophageal adenocarcinoma

Overexpression of miR-331 Indicates Poor Prognosis and Promotes Progression of Breast Cancer

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