MicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting

Zhang, X H; Geng, Guiling; Su, Bo; Liang, Chao; Wang, F; Bao, Jun

doi:10.4238/gmr.15037674

Cited by 24 publications

(12 citation statements)

References 14 publications

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“…Along with their known micro‐RNA interactions, these lncRNAs may hold potential as future experimental targets. For instance, lncRNA LINC01225 , which was strongly positively correlated with RANKL and also 2.64‐fold higher in peri‐implantitis, is predicted to interact with miR‐338‐3p suggesting possible therapeutic value of their specific modulation.…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Liu

et al. 2019

J of Periodontal Research

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Background and Objective:Peri-implantitis is a biofilm-mediated infectious disease that results in progressive loss of implant-supporting bone. As compared to its analogue periodontitis, peri-implantitis is generally known to be more aggressive, with comparatively rapid progression and less predictable treatment outcomes, especially when advanced. An understanding of molecular mechanisms underpinning the similarities and differences between peri-implantitis and periodontitis is essential to develop novel management strategies. This study aimed to compare long non-coding RNAs (lncRNAs) and messenger RNA (mRNA) expression profiles between peri-implantitis and periodontitis.Methods: Inflamed soft tissue from peri-implantitis and periodontitis lesions, and healthy gingival tissue controls were analyzed by microarray. Cluster graphs, gene ontology (GO) analysis, and pathway analysis were performed. Quantitative real-time PCR was employed to verify microarray results. The expression levels of RANKL and OPG in the three tissue types were also evaluated, using qRT-PCR. Coding non-coding (CNC) network analyses were performed.Results: Microarray analyses revealed 1079 lncRNAs and 1003 mRNAs as differentially expressed in peri-implantitis when compared to periodontitis. The cyclooxygenase-2 pathway was the most up-regulated biological process in peri-implantitis as compared to periodontitis, whereas hemidesmosome assembly was the most down-regulated pathway. Osteoclast differentiation was relatively up-regulated, and RANKL/OPG ratio was higher in peri-implantitis than in periodontitis. Conclusions:The study demonstrated that peri-implantitis and periodontitis exhibit significantly different lncRNA and mRNA expression profiles, suggesting that osteoclast differentiation-related pathways are comparatively more active in periimplantitis. These data highlight potential molecular targets for periodontitis and peri-implantitis therapy development. K E Y W O R D SlncRNA, microarray, peri-implantitis, periodontitis, transcriptome | 343 LIU et aL.

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Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Liu

et al. 2019

J of Periodontal Research

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“…For instance, osteoclasts could secrete miRNA-21 that promoted tumor cell proliferation 17 and miRNA-378 that enhanced tumor growth, angiogenesis and cell survival 18 . Also, miRNA-338-3p was demonstrated to repress glucocorticoid-induced osteoclast differentiation and bone resorption through targeting on receptor activator for nuclear factor-κB ligand (RANKL) 19 .…”

Section: Discussionmentioning

confidence: 99%

Microarray Analysis of Differentially-expressed MicroRNAs in Acquired Middle Ear Cholesteatoma

et al. 2018

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Objectives: To analyze the miRNAs expression profiling between acquired middle ear cholesteatoma and normal skin, and to identify several novel miRNAs which may be involved in the etiopathogenesis of middle ear cholesteatoma.Methods: MiRNA microarray technology was adopted to analyze the miRNA expression profiling between acquired middle ear cholesteatoma and normal skin. qRT-PCR was used to validate selected differentially expressed miRNAs.Results: The miRNA microarray technology showed totally 44 upregulated (miRNA-21-3p, miRNA-584-5p, miRNA-16-1-3p, etc) and 175 downregulated (miRNA-10a-5p, miRNA-152-5p, miRNA-203b-5p, etc) miRNAs in cholesteatoma tissues with 2-fold change compared with normal skin. The qRT-PCR validation was in accordance with the microarray results partly: miRNA-21-3p and miRNA-16-1-3p expressed significantly higher while miRNA-10a-5p exhibited an obviously decreased expression in middle ear cholesteatoma tissues when compared with normal skin. The GO and KEGG pathway analyses provided clues that these differentially expressed miRNAs might play essential roles in the etiopathogenesis of middle ear cholesteatoma, including cell proliferation, apoptosis, cell cycle, differentiation, bone resorption and remodeling process.Conclusions: Our study suggests possible roles of differentially expressed miRNAs in the pathogenesis of middle ear cholesteatoma. Targeting on these miRNAs may provide a new strategy for cholesteatoma therapy in the future.

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“…Pituitary MicroR-449a (miR-449a) impairment induced a decrease in the level of CRH receptor 1 (CRHR1) level in low-birth-weight rats (Nemoto et al, 2015 ), and miR-449a was involved in the GC induced CRHR1 downregulation (Nemoto et al, 2013 ). GC function was related to miR-338 (Zhang et al, 2016 ) and miR-433 (Smith et al, 2016 ) in osteoclast formation. Hypothalamic miRNAs reportedly played a critical role in energy balance (Schneeberger et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142

Zhu

Chen

Meng

et al. 2017

Front. Mol. Neurosci.

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Electroacupuncture (EA) could improve the hyperactivity of the hypothalamus pituitary adrenal (HPA) axis induced by hepatectomy. However, its underlying mechanism still remains largely unclear. Here, we found that hypothalamic corticotrophin releasing hormone (CRH) modulates the function of the HPA axis, while hepatectomy induced an HPA axis disorder and EA application could regulate the hypothalamic CRH. We first demonstrated that microRNAs (miRNAs) target on CRH via bioinformatics analysis and screened them in the primary hypothalamic neurons. MicroR-142 (miR-142) and miR-376c were identified to inhibit CRH at the mRNA and protein levels, and a dual luciferase reporter assay confirmed their binding to the 3′-untranslated regions (3′-UTR) of CRH. Further analyses revealed a decrease in hypothalamic miR-142 expression in the hepatectomy rats and an increase in miR-142 and miR-376c after EA intervention. Importantly, the improvement effect of EA on the HPA axis regulatory function in hepatectomy rats was blocked by miR-142 antagomir. Our findings illustrated that EA could up-regulate hypothalamic miR-142 expression and decrease the CRH level to alleviate the hyperactivity of the HPA axis induced by hepatectomy.

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MicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting

Cited by 24 publications

References 14 publications

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Microarray Analysis of Differentially-expressed MicroRNAs in Acquired Middle Ear Cholesteatoma

Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142

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