MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine cumulus cells

Pan, Bo; Toms, Derek; Shen, Wei; Li, Julang

doi:10.1152/ajpendo.00480.2014

Cited by 91 publications

(56 citation statements)

References 65 publications

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“…A putative involvement of miR-378 in regulating luteal cell survival in bovine has been suggested but not proven [23]. However, in the pig, miR-378 targets aromatase and progesterone receptor in granulosa cells and regulates both ovarian estradiol production and oocyte maturation [24], [25], [26]. Finally, another of the miRNAs investigated in our study, mir-222, may reportedly regulate steroidogenesis of granulosa cells [27].…”

Section: Discussionmentioning

confidence: 64%

A miRNA target network putatively involved in follicular atresia

Donadeu

Mohammed

Ioannidis

2017

Domestic Animal Endocrinology

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In a previous microarray study, we identified a subset of micro RNAS (miRNAs), which expression was distinctly higher in atretic than healthy follicles of cattle. In the present study, we investigated the involvement of those miRNAs in granulosa and theca cells during atresia. Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) confirmed that miR-21-5p/-3p, miR-150, miR-409a, miR-142-5p, miR-378, miR-222, miR-155, and miR-199a-5p were expressed at higher levels in atretic than healthy follicles (9–17 mm, classified based on steroidogenic capacity). All miRNAs except miR-21-3p and miR-378 were expressed at higher levels in theca than granulosa cells. The expression of 13 predicted miRNA targets was determined in follicular cells by RT-qPCR, revealing downregulation of HIF1A, ETS1, JAG1, VEGFA, and MSH2 in either or both cell types during atresia. Based on increases in miRNA levels simultaneous with decreases in target levels in follicular cells, several predicted miRNA target interactions were confirmed that are putatively involved in follicular atresia, namely miR-199a-5p/miR-155-HIF1A in granulosa cells, miR-155/miR-222-ETS1 in theca cells, miR-199a-5p-JAG1 in theca cells, miR-199a-5p/miR-150/miR-378-VEGFA in granulosa and theca cells, and miR-155-MSH2 in theca cells. These results offer novel insight on the involvement of miRNAs in follicle development by identifying a miRNA target network that is putatively involved in follicle atresia.

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Section: Discussionmentioning

confidence: 64%

A miRNA target network putatively involved in follicular atresia

Donadeu

Mohammed

Ioannidis

2017

Domestic Animal Endocrinology

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“…It is noted that some miRNAs sponged by circRNAs have been shown to control cumulus cell function, oocyte maturation, and early embryo development. For example, miRNA-224 targeted by circRNA3798 in cumulus cells (Li et al, 2017b), miRNA-21 bound by circRNA193 (Wright et al, 2016), and miRNA-378 bound by circRNA2982 (Pan et al, 2015) in oocytes. Altogether, these results demonstrate that circRNAs identified in both porcine cumulus cells and oocytes could have similar effects of miRNA sponge as those observed in other cellular contexts.…”

Section: Resultsmentioning

confidence: 99%

Circular RNA profiling in the oocyte and cumulus cells reveals thatcircARMC4is essential for porcine oocyte maturation

Cao

Gao

et al. 2019

Preprint

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Thousands of circular RNAs (circRNAs) have been recently discovered in cumulus cells and oocytes from several species. However, the expression and function of circRNA during porcine oocyte meiotic maturation have been never examined. Here, we separately identified 7,067 and 637 circRNAs in both the cumulus cell and the oocyte via deep sequencing and bioinformatic analysis. Further analysis revealed that a faction of circRNAs is differentially expressed (DE) in a developmental stage-specific manner. The host genes of DE circRNAs are markedly enriched to multiple signaling pathways associated with cumulus cell function and oocyte maturation. Additionally, most DE circRNAs harbor several miRNA targets, suggesting that these DE circRNAs potentially act as miRNA sponge. Importantly, we found that maternal circARMC4 knockdown by siRNA microinjection caused a severely impaired chromosome alignment, and significantly inhibited first polar body extrusion and early embryo development. Taken together, these results demonstrate for the first time that circRNAs are abundantly and dynamically expressed in a developmental stage-specific manner in cumulus cells and oocytes, and maternally expressed circARMC4 is essential for porcine oocyte meiotic maturation and early embryo development.

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“…Different miRNA expression levels were reported during follicle development in different mammalian species with potential involvement in follicle growth, ovulation, steroidogenesis, and follicular‐luteal transition (Donadeu, Schauer, & Sontakke, ). Recently, functional analysis studies in porcine oocytes reported specific miRNAs, such as miR‐378 (Pan, Toms, Shen, & Li, ), miR‐21 (Wright, Hale, Yang, Njoka, & Ross, ), miR‐224 (X. Li, Wang, Sheng, & Wang, ), and miR‐574 (Pan, Toms, & Li, ) regulate oocyte maturation by targeting specific transcripts.…”

Section: Discussionmentioning

confidence: 99%

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

Gad

Němcová

Murín

et al. 2019

Molecular Reproduction Devel

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Oocyte developmental competence is acquired during folliculogenesis and regulated by complex molecular mechanisms. Several molecules are involved in these mechanisms, including microRNAs (miRNAs) that are essential for oocyte‐specific processes throughout the development. The objective of this study was to identify the expression profile of miRNAs in porcine oocytes derived from follicles of different sizes using RNA deep sequencing. Oocytes were aspirated from large (LO; 3–6 mm) or small (SO; 1.5–1.9 mm) follicles and tested for developmental competence and chromatin configurations. Small RNA libraries were constructed from both groups and then sequenced in an Illumina NextSeq. 500. Oocytes from the LO group exhibited higher developmental competence and different chromatin configuration compared with oocytes from the SO group. In total, 167 and 162 known miRNAs were detected in the LO and SO groups, respectively. MiR‐205, miR‐16, miR‐148a‐3p, and miR‐125b were among the top 10 highly expressed miRNAs in both groups. Eight miRNAs were differentially expressed (DE) between both groups. Target gene prediction and pathway analysis revealed 46 pathways that were enriched with miRNA‐target genes. The oocyte meiosis pathway and signaling pathways including FoxO, PI3K‐Akt, and cAMP were predictably targeted by DE miRNAs. These results give more insights into the potential role of miRNAs in regulating the oocyte development.

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MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine cumulus cells

Cited by 91 publications

References 65 publications

A miRNA target network putatively involved in follicular atresia

A miRNA target network putatively involved in follicular atresia

Circular RNA profiling in the oocyte and cumulus cells reveals thatcircARMC4is essential for porcine oocyte maturation

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

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