MicroRNA‑379 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by targeting tumor protein D52

Zhao, Xiaojun; Chu, Jiusheng

doi:10.3892/etm.2018.6302

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…miR-449b-5p suppressed NPC cells malignant phenotype in vitro by inhibiting the expression TPD52. In particular, Zhao et al 31 illustrated that TPD52 knockdown remarkably restrained NPC cells proliferation, invasion and migration. Our paper demonstrated that TPD52 was highly expressed in tumor tissues of NPC patients.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0007637 Facilitates Nasopharyngeal Carcinoma Progression by Sponging miR-636/TPD52 Axis

Wang¹,

Li²,

Pan³

et al. 2021

CMAR

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Purpose Hsa_circ_0007637 was discovered to be differentially expressed in nasopharyngeal carcinoma (NPC). However, the exact function and mechanism of Hsa_circ_0007637 on NPC have not been studied. This study firstly researched the function and mechanism of Hsa_circ_0007637 on NPC progression. Methods Hsa_circ_0007637, miR-636 and TPD52 expressions in 80 NPC patients were detected by quantitative real-time polymerase chain reaction. Hsa_circ_0007637 effect on NPC cell proliferation, apopticosis, invasion and migration was investigated by cell counting kit-8 assay, flow cytometry, transwell experiment and wound healing assay accordingly. Dual-luciferase reporter gene assay, RNA immunoprecipitation experiment and RNA fluorescence in situ hybridization experiment were performed to identify the binding between Hsa_circ_0007637 and miR-636. Dual-luciferase reporter gene assay and RNA pull down assay were conducted to verify the binding between miR-636 and TPD52. TPD52 protein expression in NPC cells was determined by Western blot. In vivo study was performed using nude mice. Immunohistochemistry was performed to assess TPD52 and Ki67 expression in tissues. Results Hsa_circ_0007637 was overexpressed in NPC tissues and cells. High Hsa_circ_0007637 expression predicted a poor outcome for NPC patients. Hsa_circ_0007637 knockdown decreased proliferation, invasion, migration and increased apoptosis of NPC cells ( P < 0.01). Hsa_circ_0007637 could enhance TPD52 expression via sponging miR-636. miR-636 overexpression or TPD52 knockdown weakened the promoting effect of Hsa_circ_0007637 on NPC cells malignant phenotype ( P < 0.01). Hsa_circ_0007637 knockdown suppressed NPC cells growth in vivo ( P < 0.01). Conclusion Hsa_circ_0007637 facilitates NPC progression by sponging miR-636/TPD52 axis.

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Section: Discussionmentioning

confidence: 99%

Hsa_circ_0007637 Facilitates Nasopharyngeal Carcinoma Progression by Sponging miR-636/TPD52 Axis

Wang¹,

Li²,

Pan³

et al. 2021

CMAR

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“…Previous studies reported the relationship between miR-379 and various diseases. The majority of these studies suggest that miR-379 plays tumor suppressive role in many types of carcinomas, including nasopharyngeal carcinoma, cervical cancer, lung cancer, gastric cancer, hepatocellular carcinoma, bladder cancer, and osteosarcoma [108][109][110][111][112][113]. With regard to metabolic disorders as described above, de Guia et al revealed a relationship between miR-379 and lipid homeostasis dysregulation [65].…”

Section: Discussionmentioning

confidence: 99%

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

et al. 2020

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Introduction Non-alcoholic fatty liver disease (NAFLD) has a wide spectrum, eventually leading to cirrhosis and hepatic carcinogenesis. We previously reported that a series of microRNAs (miR-NAs) mapped in the 14q32.2 maternally imprinted gene region (Dlk1-Dio3 mat) are related to NAFLD development and progression in a mouse model. We examined the suitability of miR-379, a circulating Dlk1-Dio3 mat miRNA, as a human NAFLD biomarker. Methods Eighty NAFLD patients were recruited for this study. miR-379 was selected from the putative Dlk1-Dio3 mat miRNA cluster because it exhibited the greatest expression difference between NAFLD and non-alcoholic steatohepatitis in our preliminary study. Real-time PCR was used to examine the expression levels of miR-379 and miR-16 as an internal control. One patient was excluded due to low RT-PCR signal. Results Compared to normal controls, serum miR-379 expression was significantly up-regulated in NAFLD patients. Receiver operating characteristic curve analysis suggested that miR-379 is a suitable marker for discriminating NAFLD patients from controls, with an area under the PLOS ONE |

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“…MiR-379, which was also upregulated, is an onco-suppressor miRNA. MiR-379 negatively regulates cell proliferation, migration and invasion in several human cancers, including nasopharyngeal carcinoma 30 , cervical carcinoma 31 , gastric cancer 32 and bladder cancer 33 , by targeting tumor protein D52 ( TPD52 ) , V-crk avian sarcoma virus CT10 oncogene homolog-like ( CRKL ), focal adhesion kinase ( FAK) and mouse double minute 2 (MDM2).…”

Section: Discussionmentioning

confidence: 99%

miRNA profiles of canine cutaneous mast cell tumours with early nodal metastasis and evaluation as potential biomarkers

Zamarian

Ferrari

Stefanello

et al. 2020

Sci Rep

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Cutaneous mast cell tumours (MCTs) are common skin neoplasms in dogs. MicroRNAs (miRNAs) are post-transcriptional regulators involved in several cellular processes, and they can function as tumour promoters or suppressors. However, the role of miRNAs in canine MCTs has not yet been elucidated. Thus, the current study aimed to characterize miRNA profiles and to assess their value as biomarkers for MCTs. miRNA expression profiles were assessed in formalin-fixed, paraffin-embedded samples by next-generation sequencing. Ten samples were MCT tissues, and 7 were healthy adjacent tissues. Nine dysregulated miRNAs (DE-miRNAs) were then validated using RT-qPCR in a larger group of MCT samples, allowing the calculation of ROC curves and performance of multiple factor analysis (MFA). Pathway enrichment analysis was performed to investigate miRNA biological functions. The results showed that the expression of 63 miRNAs (18 up- and 45 downregulated) was significantly affected in MCTs. Five DE-miRNAs, namely, miR-21-5p, miR-92a-3p, miR-338, miR-379 and miR-885, were validated by RT-qPCR. The diagnostic accuracy of a panel of 3 DE-miRNAs—miR-21, miR-379 and miR-885—exhibited increased efficiency in discriminating animals with MCTs (AUC = 0.9854) and animals with lymph node metastasis (AUC = 0.8923). Multiple factor analysis revealed clusters based on nodal metastasis. Gene Ontology and KEGG analyses confirmed that the DE-miRNAs were involved in cell proliferation, survival and metastasis pathways. In conclusion, the present study demonstrated that the miRNA expression profile is changed in the MCT microenvironment, suggesting the involvement of the altered miRNAs in the epigenetic regulation of MCTs and identifying miR-21, miR-379 and miR-885 as promising biomarkers.

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MicroRNA‑379 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by targeting tumor protein D52

Cited by 11 publications

References 32 publications

Hsa_circ_0007637 Facilitates Nasopharyngeal Carcinoma Progression by Sponging miR-636/TPD52 Axis

Hsa_circ_0007637 Facilitates Nasopharyngeal Carcinoma Progression by Sponging miR-636/TPD52 Axis

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

miRNA profiles of canine cutaneous mast cell tumours with early nodal metastasis and evaluation as potential biomarkers

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