MicroRNA-411 inhibits malignant biological behaviours of colorectal cancer cells by directly targeting PIK3R3

Zhao, Jian; Xu, Jian; Zhang, Rui

doi:10.3892/or.2017.6135

Cited by 13 publications

(16 citation statements)

References 40 publications

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“…PIK3R3 was observed to be overexpressed in various types of human cancer, including ovarian cancer (32), colorectal cancer (33), glioma (34) and lung cancer (35). A number of previous studies demonstrated that PIK3R3 may be involved in a variety of cellular processes, including cell growth, apoptosis, migration, invasion, metastasis and epithelial-mesenchymal transition (28,36,37). Hence, targeting PIK3R3 may represent as a novel therapeutic method for cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑601 serves as a potential tumor suppressor in hepatocellular carcinoma by directly targeting PIK3R3

Song

Zhuang

et al. 2019

Mol Med Report

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Recently, microRNAs (miRNAs) have been acknowledged as important regulators of hepatocarcinogenesis and tumor progression. Therefore, identifying the underlying molecular mechanisms of miRNAs in the occurrence and development of hepatocellular carcinoma (HCC) may be important for understanding the pathogenesis of HCC and aid the identification of potential therapeutic strategies. In the present study, miRNA (miR)-601 was significantly downregulated in HCC tissues and cell lines; low miR-601 expression was strongly associated with tumor, node and metastasis staging and lymph node metastasis of patients with HCC. In addition, the overexpression of miR-601 expression significantly inhibited the proliferation and invasion of HCC cells. Regarding the underlying mechanism, phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was predicted to be a direct target of miR-601 in HCC cells. Furthermore, restoration of PIK3R3 expression in these cells counteracted the inhibitory effects of miR-601 on cell proliferation and invasion in HCC. Notably, miR-601 overexpression inhibited the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway in HCC via the regulation of PIK3R3. Collectively, these results demonstrated that miR-601 may inhibit the progression of HCC by directly targeting PIK3R3 and regulating the AKT/mTOR signaling pathway. Therefore, miR-601 may be an effective therapeutic target for the treatment of patients with HCC.

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Section: Discussionmentioning

confidence: 99%

“…Recent studies demonstrated that PIK3R3 is involved in the regulation of the AKT/mTOR signaling pathway (25)(26)(27)(28). Therefore, the present study investigated whether miR-601 may affect the AKT/mTOR signaling pathway in HCC cells.…”

Section: Mir-601 Inactivates the Akt/mtor Signaling Pathway In Hcc Cementioning

confidence: 93%

MicroRNA‑601 serves as a potential tumor suppressor in hepatocellular carcinoma by directly targeting PIK3R3

Song

Zhuang

et al. 2019

Mol Med Report

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“…MiR-411 is upregulated in hepatocellular carcinoma, 30 lung cancer, 31,32 and osteosarcoma; 33 on the contrary, it is downregulated in breast cancer, 34,35 renal cell cancer, 36 colorectal cancer, 37 cervical cancer, 38 and bladder cancer. 39,40 Nevertheless, whether miR-411 is dysregulated in ESCC and whether its dysregulation contributes to ESCC oncogenesis has been unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Among these candidates, miR-411 ( Figure 3A) was chosen for subsequent validation, based on its crucial roles in oncogenesis and tumor progression. [30][31][32][33][34][35][36][37][38][39][40] The luciferase reporter assay was carried out to gain more insights into the potential interaction between SNHG8 and miR-411. The results indicated that miR-411 mimics-mediated upregulation of miR-411 ( Figure 3B, P<0.05) significantly weakened the luciferase activity of the SNHG8-wt plasmid in Eca109 and TE-1 cells (P<0.05) but had no such effect on the SNHG8mut plasmid ( Figure 3C).…”

Section: Snhg8 Acts As a Sponge For Mir-411 In Esccmentioning

confidence: 99%

<p>SNHG8 is upregulated in esophageal squamous cell carcinoma and directly sponges microRNA-411 to increase oncogenicity by upregulating KPNA2</p>

Song¹,

Song²,

Lu³

et al. 2019

OTT

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Background: The long noncoding RNA, small nucleolar RNA host gene 8 (SNHG8), is upregulated in multiple human cancer types. However, whether SNHG8 is aberrantly expressed in esophageal squamous cell carcinoma (ESCC) and its biological functions have yet to be elucidated. Thus, we aimed to determine the expression status of SNHG8 in ESCC, explore the effects of SNHG8 on the oncogenicity of ESCC, and investigate the potential underlying mechanisms. Methods: SNHG8 expression in ESCC tissues and cell lines was determined via reversetranscription quantitative polymerase chain reaction. The actions of SNHG8 on the malignant characteristics of ESCC were explored using CCK-8 assay, flow-cytometric analysis, Transwell migration and invasion assays, and tumor xenografts in nude mice. Results: SNHG8 expression was significantly higher in ESCC tissues and cell lines. High SNHG8 expression was revealed to closely correlate with primary tumor invasion depth, lymph node metastases, TNM stage, and worse overall survival among patients with ESCC. Functional investigation showed that ablation of SNHG8 notably restricted ESCC cell proliferation, migration, and invasion while inducing apoptosis in vitro and hindered tumor growth in vivo. In the meantime, SNHG8 acted as a molecular sponge of microRNA-411 (miR-411) in ESCC. Furthermore, miR-411 exerted a tumor-suppressive effect on ESCC cells, and karyopherin alpha 2 (KPNA2) turned out to be a direct target gene of miR-411. Restoring KPNA2 expression neutralized the inhibitory effects of miR-411 overexpression on the malignant behaviors of ESCC cells. Moreover, silencing of miR-411 abrogated the influence of SNHG8 downregulation in ESCC cells. Conclusion: SNHG8 may play oncogenic roles in the malignancy of ESCC by sponging miR-411 to increase KPNA2 expression. The SNHG8-miR-411-KPNA2 pathway may be a novel target for the treatment of patients with ESCC and offer potential biomarkers for the diagnosis and prognosis of ESCC.

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“…Thus, miR-155 could be considered a prognostic marker for overall and disease-free survival ( 147 ). In a recent study, Zhao et al ( 148 ) presented that miR-411 functions as a tumor-suppressive miRNA directly targeting PIK3R3 and indirectly regulating the AKT/mTOR signaling pathway. In other words, an increase of miR-411 would result in the downregulation of PIK3R3 directly and AKT/mTOR indirectly.…”

Section: Prognostic Markersmentioning

confidence: 99%

Improving diagnosis, prognosis and prediction by using biomarkers in CRC patients (Review)

et al. 2018

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Colorectal cancer (CRC) is among the most common cancers. In fact, it is placed in the third place among the most diagnosed cancer in men, after lung and prostate cancer, and in the second one for the most diagnosed cancer in women, following breast cancer. Moreover, its high mortality rates classifies it among the leading causes of cancer-related death worldwide. Thus, in order to help clinicians to optimize their practice, it is crucial to introduce more effective tools that will improve not only early diagnosis, but also prediction of the most likely progression of the disease and response to chemotherapy. In that way, they will be able to decrease both morbidity and mortality of their patients. In accordance with that, colon cancer research has described numerous biomarkers for diagnostic, prognostic and predictive purposes that either alone or as part of a panel would help improve patient's clinical management. This review aims to describe the most accepted biomarkers among those proposed for use in CRC divided based on the clinical specimen that is examined (tissue, faeces or blood) along with their restrictions. Lastly, new insight in CRC monitoring will be discussed presenting promising emerging biomarkers (telomerase activity, telomere length and micronuclei frequency).

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MicroRNA-411 inhibits malignant biological behaviours of colorectal cancer cells by directly targeting PIK3R3

Cited by 13 publications

References 40 publications

MicroRNA‑601 serves as a potential tumor suppressor in hepatocellular carcinoma by directly targeting PIK3R3

MicroRNA‑601 serves as a potential tumor suppressor in hepatocellular carcinoma by directly targeting PIK3R3

<p>SNHG8 is upregulated in esophageal squamous cell carcinoma and directly sponges microRNA-411 to increase oncogenicity by upregulating KPNA2</p>

Improving diagnosis, prognosis and prediction by using biomarkers in CRC patients (Review)

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