MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare

Abstract

With the development of information technology, the concept of smart healthcare has gradually come to the fore. Smart healthcare uses a new generation of information technologies, such as the Internet of Things (loT), big data, cloud computing, and artificial intelligence, to transform the traditional medical system in an all-around way, making healthcare more efficient, more convenient, and more personalized. miRNAs can regulate the proliferation, differentiation, and apoptosis of human cells. Relevant studies have also shown that miRNAs may play a key role in the occurrence and development of myocardial ischemia-reperfusion injury (MIRI). This study aims to explore the effects of miR-489 in MIRI. In this study, miR-489 expression in a myocardial ischemia-reperfusion animal model and H9C2 cells induced by H/R was detected by qRT-PCR. The release of lactate dehydrogenase (LDH) and the activity of creatine kinase (CK) was detected after miR-489 knockdown in H9C2 cells induced by H/R. The apoptosis of H9C2 cells and animal models were determined by ELISA. The relationship between miR-489 and SPIN1 was verified by a double fluorescence reporter enzyme assay. The expression of the PI3K/AKT pathway-related proteins was detected by Western blot. Experimental results showed that miR-489 was highly expressed in cardiac muscle cells of the animal model and in H9C2 cells induced by H/R of the myocardial infarction group, which was positively associated with the apoptosis of cardiac muscle cells with ischemia-reperfusion. miR-489 knockdown can reduce the apoptosis of cardiac muscle cells caused by ischemia-reperfusion. In downstream targeting studies, it was found that miR-489 promotes the apoptosis of cardiac muscle cells after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. In conclusion, high expression of miR-489 is associated with increased apoptosis of cardiac muscle cells after ischemia-reperfusion, which can promote the apoptosis after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. Therefore, miR-489 can be one of the potential therapeutic targets for reducing the apoptosis of cardiac muscle cells after ischemia-reperfusion.

Psoriasis and cardiovascular disease risk in European and East Asian populations: evidence from meta-analysis and Mendelian randomization analysis

“…HLA-B*35-linked haplotypes are more prevalent in CAD subjects as compared with that of healthy age- and sex-matched controls [ 56 ]. Additionally, miR-489 can enhance cardiac muscle cell apoptosis following ischemia–reperfusion via dampening of the SPIN1-mediated PI3K/AKT cascade [ 57 ]. Based on a large GWAS consortia, our MR results present convincing evidence of a causative role for psoriasis in CAD and MI, despite the unknown confounders in observational studies, underlining the importance of treating psoriasis in reducing the risk of the onset of CAD and MI.…”

Expression Analysis of Moritella viscosa-Challenged Atlantic Salmon Identifies Disease-Responding Genes, MicroRNAs and Their Predicted Target Genes and Pathways

“…All of these have been shown as involved in regulation of immune responses, angiogenesis, or wound healing. The three downregulated miRNAs were; miR-137 (angiogenesis and muscle regeneration [ 63 , 64 ]), miR-218 (regulation of cytokines, chemokines and interferon production, hypoxia-responding [ 65 , 66 , 67 ]) and mir-489 (apoptosis and wound healing [ 68 , 69 , 70 ]). Similarly, the three upregulated miRNAs have been reported as involved in regulation of disease relevant responses; miR-730 is an Actinopterygii-specific miRNA responding to bacterial infection in Zebrafish [ 52 , 71 , 72 ]), miR-144 regulates apoptosis/cell cycle control [ 73 ] while the teleost specific miR-2188 responds to viral infection and is likely involved in macrophage differentiation [ 25 , 74 ].…”

Retracted: MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare

“…Journal of Healthcare Engineering has retracted the article titled "MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare" [1] due to concerns that the peer review process has been compromised. Following an investigation conducted by the Hindawi Research Integrity team [2], signifcant concerns were identifed with the peer reviewers assigned to this article; the investigation has concluded that the peer review process was compromised.…”