microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Chen, Shuo; Chen, Xi; Xiu, Yin-Ling; Sun, Kaixuan; Zong, Zhi‐Hong; Zhao, Yang

doi:10.1186/s13048-014-0084-4

Cited by 35 publications

(30 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A deeper insight into the molecular mechanism of drug resistance is the key to resolving the issue. Several researchers have proved that expression dysregulation of miRNA in tumor cells could influence their sensitivity of the tumor to drugs by regulating target gene expression 20–22. Therefore, target-regulating miRNA is the advanced pathway for overcoming chemotherapy resistance of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Targeted regulation of MiR-98 on E2F1 increases chemosensitivity of leukemia cells K562/A02

Huang¹,

Hong²,

Hu³

et al. 2017

OTT

View full text Add to dashboard Cite

BackgroundmiRNA is a microRNA that negatively regulates protein expression at post-transcriptional or translational level. It is widely involved in the pathogenesis of tumors. miR-98 belongs to the let-7 family, and its overexpression can increase the sensitivity to drugs in solid cancer cells. However, the function of miR-98 in leukemia is still unclear. In this study, the effect of miR-98 on drug resistance and proliferation of leukemia cells were investigated.MethodsReal-time quantitative polymerase chain reaction analyzed the expression difference between miR-98 and E2F1 in leukemia cell lines, K562 and K562/A02. The downstream target gene of miR-98 was predicted by TargetScan; K562/A02 was transiently transfected with miR-98 mimic to upregulate the expression of miR-98; real-time quantitative polymerase chain reaction and Western blot were used to analyze the expression alterations of E2F1; cell counting kit-8 was used to evaluate the influence on K562/A02 proliferation and sensitivity to chemotherapeutic drugs; meanwhile, Western blot was used to analyze the expression of p21, Bax, matrix metalloproteinase 9 and ABCG2 proteins.ResultsE2F1 is one of the target genes of miR-98 proved by bioinformatics. Compared with the K562, the level of miRNA-98 expression was decreased in K562/A02, but the level of E2F1 expression was upregulated. Leukemia cell line K562/A02 was transfected with miR-98 mimic to upregulate the expression of miR-98, the expression of E2F1 was significantly decreased. After upregulating the miR-98 expression in K562/A02, the proliferation was weakened, and the sensitivity to chemotherapy was increased. Western blot showed that upregulated miR-98 expression increased the levels of p21 and BAX proteins in K562/A02 cells, and decreased the levels of matrix metalloprotease 9 and ABCG2 proteins, which were significantly different compared with those before miR-98 mimic transfection.ConclusionIn the leukemia drug-resistant cell line K562/A02, the targeted upregulated expression of miR-98 could decrease the proliferation of leukemia cells and improve the sensitivity to chemotherapeutics by inhibiting E2F1 expression. miR-98 might be a potential target for overcoming leukemia multidrug resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Targeted regulation of MiR-98 on E2F1 increases chemosensitivity of leukemia cells K562/A02

Huang¹,

Hong²,

Hu³

et al. 2017

OTT

View full text Add to dashboard Cite

show abstract

“…Previous studies showed the role of Hsa-miR-490 as potential drug resistance in ovarian cancer [147] and as a potential novel biomarker for diagnosing of colorectal cancer [148]. …”

Section: Discussionmentioning

confidence: 99%

How interacting pathways are regulated by miRNAs in breast cancer subtypes

Cava

Colaprico²,

Bertoli

et al. 2016

BMC Bioinformatics

View full text Add to dashboard Cite

BackgroundAn important challenge in cancer biology is to understand the complex aspects of the disease. It is increasingly evident that genes are not isolated from each other and the comprehension of how different genes are related to each other could explain biological mechanisms causing diseases. Biological pathways are important tools to reveal gene interaction and reduce the large number of genes to be studied by partitioning it into smaller paths. Furthermore, recent scientific evidence has proven that a combination of pathways, instead than a single element of the pathway or a single pathway, could be responsible for pathological changes in a cell.ResultsIn this paper we develop a new method that can reveal miRNAs able to regulate, in a coordinated way, networks of gene pathways. We applied the method to subtypes of breast cancer. The basic idea is the identification of pathways significantly enriched with differentially expressed genes among the different breast cancer subtypes and normal tissue. Looking at the pairs of pathways that were found to be functionally related, we created a network of dependent pathways and we focused on identifying miRNAs that could act as miRNA drivers in a coordinated regulation process.ConclusionsOur approach enables miRNAs identification that could have an important role in the development of breast cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s12859-016-1196-1) contains supplementary material, which is available to authorized users.

show abstract

“…miRNA treatment includes miRNA that substitutes low expression and miRNA that inhibits overexpression. Previous studies have used agomirs and antagomirs as analogues and inhibitors, respectively, of miRNAs for the treatment of cancer, and a large number of experiments in vivo have been conducted (23)(24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines

Liu

et al. 2016

Oncology Letters

View full text Add to dashboard Cite

Abstract. The present study aimed to explore the expression of microRNA (miRNA or miR) in drug-resistant and drug-sensitive ovarian cancer cell lines, and to seek the potential therapeutic target of ovarian cancer drug-resistant mechanism in order to improve drug resistance by altering miRNA levels. The drug-resistant characteristics of SKOV3/DDP, SKOV3, COC1/DDP and COC1 cell lines were studied. The miRNAs that were differentially expressed between cisplatin-resistant cells and its parental cells in ovarian cancer were screened with a miRNA chip. The effect of miRNAs was detected, and their drug-resistant mechanism was investigated by transfection and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide methods. Among the expression screening of miRNAs, 41 mRNAs, including Homo sapiens (hsa)-miR-30a-5p and hsa-miR-34c-5p, were highly expressed in the drug-resistant cells, whereas 44 miRNAs, including hsa-miR-96-5p and hsa-miR-200c-3p, were lowly expressed. The expression levels of hsa-miR-30a-5p in two types of ovarian cancer chemotherapy-resistant cell lines were significantly higher than those in chemotherapy-sensitive cell lines, which was associated with ovarian cancer chemotherapy resistance. In conclusion, high expression of miRNA-30a-5p was able to promote cell growth and colony forming ability, and enhance cell migration and invasion. Thus, miRNA-30a-5p is expected to become a meaningful novel target for ovarian cancer resistant treatment.

show abstract

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Cited by 35 publications

References 31 publications

Targeted regulation of MiR-98 on E2F1 increases chemosensitivity of leukemia cells K562/A02

Targeted regulation of MiR-98 on E2F1 increases chemosensitivity of leukemia cells K562/A02

How interacting pathways are regulated by miRNAs in breast cancer subtypes

Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines

Contact Info

Product

Resources

About