MicroRNA‑503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12

Abstract

Numerous studies have demonstrated that microRNAs (miRNAs or miRs) are abnormally expressed in retinoblastoma (RB). miRNAs may serve a role in oncogene or tumor-suppressor activity in RB genesis and development by modulating various biological processes. miRNAs therefore, may be effective therapeutic targets for miRNA-based therapy in patients with RB. Recently it has been revealed that miR-503 may serve a role in various types of human cancer. However, the expression and functional roles of miR-503 are rarely reported in RB. In the current study, the expression of miR-503 was significantly upregulated in RB tissues and cell lines. In addition, Cell Counting Kit-8 and in vitro invasion assays were performed to assess cell proliferation and invasion, respectively. The results of the present study revealed that miR-503 inhibition impeded RB in vitro cell proliferation and invasion. Furthermore, protein tyrosine phosphatase nonreceptor type 12 (PTPN12) was demonstrated to be a direct target gene of miR-503 in RB cells. PTPN12 overexpression also led to the downregulation of miR-503 in RB cell proliferation and invasion. PTPN12 knockdown could therefore abrogate the effects of miR-503 downregulation in RB cells. In conclusion, the results demonstrated that miR-503 may serve a role in RB oncogenic activity progression by directly targeting PTPN12. Therefore, miR-503 may be a target for effective therapy in patients with RB.

The Causes and Consequences of miR-503 Dysregulation and Its Impact on Cardiovascular Disease and Cancer

“…miR-Taken together, miR-503 expression level varies in different types of cancer. However, except for the inconsistent results of colorectal cancer and glioblastoma multiforme, miR-503 has been found to be downregulated in most of cancer types, but upregulated in few cancer types, such as retinoblastoma (Zhao et al, 2009;Cheng and Liu, 2019), ESCC (Wu et al, 2018) and adrenocortical carcinoma (Ozata et al, 2011). miR-503 can act as "onco-miR" or tumor suppressor in different types of cancer.…”

“…In vitro, miR-503 was also lower in osteosarcoma cells than that in normal osteoblasts cells, and overexpression of miR-503 suppressed the proliferation and invasion of osteosarcoma U2OS cells, thus, it acted as a tumor suppressor in osteosarcoma via inhibiting its target, IGF-1R. However, in retinoblastoma (RB)(Cheng and Liu, 2019), miR-503 was greatly expressed in RB tissues, and acted as an "onco-miR" by targeting PTPN12.Frontiers in Pharmacology | www.frontiersin.org March 2021 | Volume 12 | Article 629611…”

The Causes and Consequences of Mir-503 Dysregulation and Its Impact on Cardiovascular Disease and Cancer

“…Taken together, miR-503 expression level varies in different types of cancer. However, except for the inconsistent results of colorectal cancer and glioblastoma multiforme, miR-503 has been found to be downregulated in most of cancer types, but upregulated in few cancer types, such as retinoblastoma [22,73], ESCC [71] and adrenocortical carcinoma [74]. miR-503 can act as "onco-miR" or tumor suppressor in different types of cancer.…”

“…In vitro, miR-503 was also lower in osteosarcoma cells than that in normal osteoblasts cells, and overexpression of miR-503 suppressed the proliferation and invasion of osteosarcoma U2OS cells, thus, it acted as a tumor suppressor in osteosarcoma via inhibiting its target, IGF-1R. However, in retinoblastoma(RB) [73], miR-503 was greatly www.videleaf.com expressed in RB tissues, and acted as an "onco-miR" by targeting PTPN12.…”

MicroRNA in Ophthalmology

“…А именно, ингибирование ее in vitro препятствует размножению и инвазии клеток РБ. Показано, что miRNA-503 может играть роль в прогрессировании активности РБ, непосредственно воздействуя на PTPN12 (прямая мишень miRNA-503) [46]. Дальнейшие исследования подтвердили значительную роль miRNA в онкогенной или опухолеподавляющей активности при развитии РБ.…”