MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway

Abstract

Preeclampsia is a pregnancy disorder that is primarily associated with maternal and neonatal or fetal morbidity and mortality. The discovery of dysregulated microRNAs (miRs) and their roles in preeclampsia has provided new insight into the mechanisms involved in pregnancy-related disorders. In the present study, quantitative PCR demonstrated that the expression levels of miR-524-5p were lower in patients with preeclampsia than those in normal pregnant women. Cell Counting Kit-8 and Transwell assays indicated that overexpression of miR-524-5p promoted the proliferation and invasion of HTR-8/SVneo cells, whereas inhibition of miR-524-5p suppressed HTR-8/SVneo cell proliferation and invasion. Furthermore, NUMB endocytic adaptor protein (NUMB), a negative regulator of the Notch signaling pathway and a target gene of miR-524-5p, limited the effects of miR-524-5p on HTR-8/SVneo cell invasion and migration. The present study demonstrated that miR-524-5p regulated the proliferation and invasion of HTR-8/SVneo cells at least partly by targeting NUMB to regulate the Notch signaling pathway.

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

“…However, the role of individual C19MC members has not been well established. Only few studies have investigated the role of specific C19MC members in trophoblast function and done so using only a single cell line ( Anton et al, 2015 ; Ding et al, 2015 ; Zhang et al, 2021 ; Zheng et al, 2021 ).…”

“…In PE, many members of the C19MC were shown to be dysregulated with the majority of them being upregulated ( Hromadnikova et al, 2013 ; Miura et al, 2015 ; Inno et al, 2021 ). For example, miR-512-5p, miR-515-5p, miR-517a/b/c, miR-518a/e/f-5p, miR-519a/b/c/e-5p, miR-519days/c-3p, miR-520a/b/c-5p and miR-520a-3p were consistently upregulated in PE ( Anton et al, 2015 ; Ding et al, 2015 ; Zhang M. et al, 2016 ; Inno et al, 2021 ) while miR-524-5p and miR-525-5p were consistently downregulated ( Zhang et al, 2021 ; Zheng et al, 2021 ). Given the large number of miRNAs within the C19MC cluster, the differential regulation of at least some cluster members and their potentially varying function, it can be expected that individual miRNAs in this cluster have distinct expression profiles throughout gestation.…”

Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR‐188‐3p/GRHL2 axis

“…18,19 For example, miR-524-5p was down-regulated in the placenta tissue of PE patients, and miR-524-5p overexpression facilitated the proliferation and invasion of HTR-8/SVneo cells. 20 MiR-188-3 had been reported to be lowly expressed in the plasma of PE patients, 21 but its role in PE was unclear.…”

“…And studies had shown that the differential expression of miRNA was related to the differentiation, migration and invasion of trophoblast cells 18,19 . For example, miR‐524‐5p was down‐regulated in the placenta tissue of PE patients, and miR‐524‐5p overexpression facilitated the proliferation and invasion of HTR‐8/SVneo cells 20 . MiR‐188‐3 had been reported to be lowly expressed in the plasma of PE patients, 21 but its role in PE was unclear.…”

Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia

“…Preeclampsia (PE) is one of the hypertensive disorders during pregnancy [ 1 ]. It has been proved that PE greatly contributes to the increasing maternal and perinatal morbidity and mortality around the world.…”