MicroRNA‑590 inhibits migration, invasion and epithelial‑to‑mesenchymal transition of esophageal squamous cell carcinoma by targeting low‑density lipoprotein receptor‑related protein 6

Abstract

MicroRNA-590 (miR-590) has been revealed as a tumor suppressor, while low-density lipoprotein receptor-related protein 6 (LRP6) is considered to act as a tumor promoter. However, their roles and underlying molecular regulatory mechanisms in esophageal squamous cell carcinoma (ESCC) have yet to be fully elucidated. Therefore, the present study aimed to investigate these mechanisms. The expression levels of miR-590 and LRP6 in human ESCC samples and cell lines were determined using reverse transcription-quantitative PCR. Bioinformatics analysis was used to predict the relationship between miR-590 and LRP6, and luciferase assay was performed to validate the relationship between these factors. Transwell assays were used to determine cell migration and invasion, while western blotting assays were used to detect the protein expression levels of LRP6, E-cadherin, N-cadherin and Vimentin. The present study demonstrated that miR-590 was downregulated and LRP6 was upregulated in ESCC tissues and cell lines. Furthermore, it was found that miR-590 overexpression and LRP6 knockdown inhibited cell migration, invasion and epithelial-to-mesenchymal transition (EMT) in ESCC cell lines. Additional mechanistic studies identified that LRP6 was a target of, and was inhibited by, miR-590. Collectively, the present findings suggested that miR-590 inhibited the invasion, migration and EMT of ESCC cells by mediating LRP6.

Long non‑coding RNA CASC9/microRNA‑590‑3p axis participates in lutein‑mediated suppression of breast cancer cell proliferation

“…miRNAs are short RNAs with a length of 20–25 nucleotides ( 39 ). They can inhibit or degrade their target mRNAs by binding to the 3′non-coding region of their target gene mRNA ( 18 , 19 ). It has been reported that miRNAs are involved in a series of important processes, such as tumor proliferation, invasion and migration ( 40 , 41 ).…”

“…Recent studies have reported that non-coding RNAs, such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs), play important roles in the development and progression of tumors (12)(13)(14). lncRNAs and miRNAs can influence the development of tumors by regulating physiological processes, such as cell proliferation and migration, apoptosis and autophagy (15)(16)(17)(18)(19). To the best of our knowledge, previous studies have not investigated whether lncRNAs and miRNAs enhance or affect the anticancer activity of lutein on breast cancer cells.…”

circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis

“…miRNAs exert their biological functions by regulating the expression of their target genes at the transcriptional or post-transcriptional level (44). For example, it has been determined that HDAC4 is regulated by miR-200b-3p, miR-206, miR-29b and miR-873-3p (45)(46)(47)(48).…”

The LINC01410/miR‐122‐5p/NDRG3 axis is involved in the proliferation and migration of osteosarcoma cells

“…MicroRNAs (miRNAs/miRs) are a kind of endogenous non‐coding small molecule single‐stranded RNA, with length generally between 19 and 24 nt; they mainly affect cell development, differentiation, proliferation, and apoptosis 25–28 . For instance, miR‐590 repressed the epithelial–mesenchymal transition (EMT) process by regulating LRP6 in esophageal squamous‐cell carcinoma 29 ; miR‐1 was proven to inhibit the malignancy of breast cancer stem cells by mediating Evi‐1 30 . MicroRNA‐122‐5p (miR‐122‐5p) acts as a tumor suppressor in ovarian, bile duct, and gastric cancers, glioma, and nasopharyngeal carcinoma 31–36 …”