MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

Tribolet, Leon; Kerr, Emily; Cowled, Christopher; Bean, Andrew G.D.; Stewart, Cameron R.; Dearnley, Megan; Farr, Ryan J.

doi:10.3389/fmicb.2020.01197

Cited by 162 publications

(117 citation statements)

References 125 publications

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“…As a result of the assay design, we expect it to be ideally‐suited for detecting short nucleic acid targets such as microRNA (18–25 mer) or short DNA barcodes released from DNA machines [32] . MicroRNAs have been identified as clinically important diagnostic biomarkers for various diseases including cancer, [33, 34] cardiovascular conditions, [35] and infectious diseases [36] …”

Section: Resultsmentioning

confidence: 99%

Enhancing the Sensitivity of Photoelectrochemical DNA Biosensing Using Plasmonic DNA Barcodes and Differential Signal Readout

et al. 2021

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Photoelectrochemical biosensors hold great promise for sensitive bioanalysis; however, similar to their electrochemical analogues, they are highly affected by the variable backgrounds caused by biological matrices. We developed a new PEC biosensing strategy that uses differential signal generation, combining signals from two separate but correlated binding events on the biosensor, for improving the limit‐of‐detection, sensitivity, and specificity of PEC DNA biosensors in biological samples. In this assay, the binding of unlabeled target DNA is followed by the capture of a signal amplification barcode featuring a plasmonic nanoparticle. The interaction of the plasmonic barcode with the semiconductive building blocks of the biosensor results in significant signal amplification, and together with differential signal processing enhances the limit of detection and sensitivity of the assay by up to 15‐ and three‐fold, respectively, compared to the previously‐used PEC assays with a single binding event, demonstrating a limit of detection of 3 fM.

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Section: Resultsmentioning

confidence: 99%

Enhancing the Sensitivity of Photoelectrochemical DNA Biosensing Using Plasmonic DNA Barcodes and Differential Signal Readout

et al. 2021

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“…The possibility and the practical aspects of using miRNAs as biomarkers have been intensively reviewed in many human diseases, such as cancers [ 30 , 31 , 32 ], rheumatic diseases [ 33 ], diabetes mellitus [ 34 , 35 ], and infectious diseases [ 36 ]. In farm animals, discovering biomarkers will be crucial for the management of disease [ 37 ].…”

Section: Mirna Biomarker Development and Potential Therapeutic Appmentioning

confidence: 99%

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

Ngoc

Dudemaine

Mathur

et al. 2021

IJMS

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MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene expression post-transcriptionally by targeting either the 3′ untranslated or coding regions of genes. They have been reported to play key roles in a wide range of biological processes. The recent remarkable developments of transcriptomics technologies, especially next-generation sequencing technologies and advanced bioinformatics tools, allow more in-depth exploration of messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs), including miRNAs. These technologies have offered great opportunities for a deeper exploration of miRNA involvement in farm animal diseases, as well as livestock productivity and welfare. In this review, we provide an overview of the current knowledge of miRNA roles in major farm animal diseases with a particular focus on diseases of economic importance. In addition, we discuss the steps and future perspectives of using miRNAs as biomarkers and molecular therapy for livestock disease management as well as the challenges and opportunities for understanding the regulatory mechanisms of miRNAs related to disease pathogenesis.

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“…However, it is still too early to develop disease-modifying therapeutics that directly target and regulate the lncRNAs, or whether lncRNAs themselves can be used as pharmaceutical molecules (Hu et al, 2020a). The technology and feasibility in this area are also immature for the earlier researched miRNAs (Tribolet et al, 2020). Moreover, siRNA-mediated treatment technology based on RNA interference has made progress.…”

Section: Conclusion and New Perspectivesmentioning

confidence: 99%

Dysregulation of lncRNAs in Rheumatoid Arthritis: Biomarkers, Pathogenesis and Potential Therapeutic Targets

et al. 2021

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Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown etiology, mainly manifested by persistent abnormal proliferation of fibroblast-like synoviocytes (FLSs), inflammation, synovial hyperplasia and cartilage erosion, accompanied by joint swelling and joint destruction. Abnormal expression or function of long noncoding RNAs (lncRNAs) are closely related to human diseases, including cancers, mental diseases, autoimmune diseases and others. The abnormal sequence and spatial structure of lncRNAs, the disorder expression and the abnormal interaction with the binding protein will lead to the change of gene expression in the way of epigenetic modification. Increasing evidence demonstrated that lncRNAs were involved in the activation of FLSs, which played a key role in the pathogenesis of RA. In this review, the research progress of lncRNAs in the pathogenesis of RA was systematically summarized, including the role of lncRNAs in the diagnosis of RA, the regulatory mechanism of lncRNAs in the pathogenesis of RA, and the intervention role of lncRNAs in the treatment of RA. Furthermore, the activated signal pathways, the role of DNA methylation and other mechanism have also been overview in this review.

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MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

Cited by 162 publications

References 125 publications

Enhancing the Sensitivity of Photoelectrochemical DNA Biosensing Using Plasmonic DNA Barcodes and Differential Signal Readout

Enhancing the Sensitivity of Photoelectrochemical DNA Biosensing Using Plasmonic DNA Barcodes and Differential Signal Readout

miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies

Dysregulation of lncRNAs in Rheumatoid Arthritis: Biomarkers, Pathogenesis and Potential Therapeutic Targets

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