MicroRNA dataset of normal and Nosema ceranae-infected midguts of Apis cerana cerana workers

Abstract

Nosema ceranae is a widespread fungal pathogen of honeybees, which is infective to all castes in the colony, including queens, drones and workers. Nosemosis caused by N. ceranae poses a big challenge for apiculture all over the world. Here, midguts of normal and N. ceranae-infected Apis cerana cerana workers at 7 and 10 days post infection were sequenced utilizing small RNA sequencing (sRNA-seq) technology. Totally, more than 150.54 Mb raw reads were produced in this article, and over 144.26 Mb high-quality clean reads with a mean ratio of 95.83% were obtained after strict filtering and quality control. For more insight please see “Comparative identification of microRNAs in Apis cerana cerana workers' midguts responding to Nosema ceranae invasion” (Chen et al., 2019). Raw data are available in NCBI Sequence Read Archive (SRA) database under the BioProject number PRJNA487111. Our data can be used for investigating differentially expressed microRNAs (miRNAs) and piRNAs and their regulatory roles engaged in A. c. cerana response to N. ceranae infection, and for offering potential candidates for uncovering the molecular mechanisms regulating eastern honeybee-microsporidian interactions.

Pacific Biosciences long reads-based genome sequencing data from a widespread bee fungal parasite,Nosema ceranae

“…Fungal spore preparation Following the method described by Cornman et al [1] with some minor modifications [2], using Percoll discontinuous density gradient centrifugation, N. ceranae spores were previously purified from an N. ceranae-infected western honeybee colony at College of Animal Sciences (College of Bee Science) in Fujian Agriculture and Forestry University. A bit of spores was examined via PCR and confirmed to be mono-specific [3] with previously developed specific primers [4].…”

A full-length transcriptome dataset of normal andNosema ceranae-challenged midgut tissues of eastern honeybee workers

“…Cornman et al [1] with some minor modifcations [2]. A small quantity of spores was examined by PCR detection and verified to be mono-specific [3] using previously developed specific primers [4]. The purified spores were immediately used for artificial inoculation of workers in treatment groups.…”

“…The purified spores were immediately used for artificial inoculation of workers in treatment groups. Artificial inoculation of bees and preparation of midgut samples were performed following our previously established method [3,5]. (1) Workers in un-challenged groups were inoculated in an identical manner with a 50% sucrose solution without spores.…”

Long-read transcriptome data of bee fungal parasite,Nosema ceranae

“…ceranae spores were previously purified following the method described by Cornman et al [1] with some minor modifications [2]. A bit of spores was subjected to PCR detection and proved to be mono-specific [3] using previously developed specific primers [4]. The purified spores were immediately frozen in liquid nitrogen and stored at -80 °C until use.…”