MicroRNA Era: The Importance for Diagnosis and Prognosis of Adrenocortical Tumors

Bezerra, Joao E.; Latrônico, Ana Claudia

doi:10.1155/2014/381917

Cited by 7 publications

(3 citation statements)

References 58 publications

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“…MicroRNAs (miRNAs) are a varied collection of non‐protein‐coding tiny (19‐22 nucleotides) RNAs with regulatory impacts on gene expression at post‐transcriptional level . By targeting or silencing target genes, miRNAs play a crucial role in controlling the progression of tumour cells.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

Diao

Jin

Huang

et al. 2018

J Cellular Molecular Medi

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This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cytometer, transwell assay and wound‐healing assay were employed to detect cell proliferation, apoptosis and cycle, invasiveness and migration, respectively. Dual‐luciferase reporting assay was performed to confirm the targeted relationship between miR‐129‐5p and TGIF2. The effects of TGIF2 expression on cell biological functions were also investigated using the indicated methods. Tumour xenograft was applied to explore the impact of miR‐129‐5p on tumorigenesis in vivo. MiR‐129‐5p expression was down‐regulated in both glioma tissues and glioma cells, while TGIF2 expression was aberrantly higher than normal level. Dual‐luciferase reporter assay validated the targeting relation between miR‐129‐5p and TGIF2. Overexpression of miR‐129‐5p or down‐regulation of TGIF2 inhibited the proliferation, invasion and migration capacity of glioma cells U87 and U251, and meanwhile blocked the cell cycle as well as induced cell apoptosis. MiR‐129‐5p overexpression repressed the tumour development in vivo. MiR‐129‐5p and TGIF2 had opposite biological functions in glioma cells. MiR‐129‐5p could inhibit glioma cell progression by targeting TGIF2, shining light for the development of target treatment for glioma.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

Diao

Jin

Huang

et al. 2018

J Cellular Molecular Medi

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“…MicroRNAs (miRNAs) are small noncoding RNAs that play critical roles in regulating various cellular functions by transcriptional silencing (Bezerra and Latronico, 2014 ). For instance, Zhou et al ( 2017 ) proposed that miR-224 could target SMAD4 to promote colorectal cell propagation.…”

Section: Introductionmentioning

confidence: 99%

NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway

Zhang

Ling

et al. 2018

Front. Mol. Neurosci.

105

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Objective: The present study aimed to explore the association between NFIX circular RNA (circNFIX) and miR-34a-5p in glioma. Furthermore, this study investigated the influence that circNFIX has on glioma progression through the upregulation of NOTCH1 via the Notch signaling pathway by sponging miR-34a-5p.Methods: We applied five methods, CIRCexplorer2, circRNA-finder, CIRI, find-circ and MapSplice2, to screen for circRNAs with differential expression between three glioma tissue samples and three paired normal tissue samples. The GSEA software was used to confirm whether significantly different pathways were activated or inactivated in glioma tissues. The binding sites between circNFIX and miR-34a-5p were confirmed by TargetScan. QRT-PCR and western blot were used to measure the relative expression levels of circNFIX, miR-34a-5p and NOTCH and identify their correlation in glioma. RNA immunoprecipitation (RIP) validated the binding relationship between circNFIX and miR-34a-5p, while the targeted relationship between NOTCH1 and miR-34a-5p was verified by a dual luciferase reporter assay. Cell viability and mobility were examined by a CCK-8 assay and wound healing assay, and a flow cytometry assay was employed to analyze cell apoptosis. The nude mouse transplantation tumor experiment verified that si-circNFIX exerted a suppressive effect on glioma progression in vivo.Results: Twelve circRNAs were differentially expressed between the tissue types. Of those, circNFIX was the sole circRNA to be overexpressed in glioma among the five methods of finding circRNAs. In addition, the Notch signaling pathway was considerably upregulated in tumor tissues compared with the paired normal brain tissues. It was determined that circNFIX acted as a sponge of miR-34a-5p, a miRNA that targeted NOTCH1. Downregulation of circNFIX and upregulation of miR-34a-5p both inhibited cell propagation and migration. Furthermore, a miR-34a-5p inhibitor neutralized the suppressive effect of si-circNFIX on glioma cells. Si-circNFIX and miR-34a-5p mimics promoted cell apoptosis. Moreover, it was demonstrated in vivo that si-circNFIX could suppress glioma growth by regulating miR-34a-5p and NOTCH1.Conclusion: CircNFIX was markedly upregulated in glioma cells. CircNFIX could regulate NOTCH1 and the Notch signaling pathway to promote glioma progression by sponging miR-34a-5p via the Notch signaling pathway. This finding provided a deeper insight into the function of circNFIX in human glioma cancer progression.

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“…В исследовании АКО у детей [17] с использованием технологии SNP-микрочипов были обнаружены повреждения в локусах онкогенов (MYC, MDM2, PDGFRA, KIT, MCL1, BCL2L1) и генов-супрессоров опухолевого роста (TP53, RB1, RPH3AL). Новые возможности дифференциальной диагностики АКО и АКК появились при использовании геномного и транскриптомного анализа, анализа микроРНК и метилирования ДНК [18,19]. Например, была выявлена связь гиперрегуляции IGF2, гиперэкспрессии mir-483 (3p и 5p) и гипорегуляции mir-195 с развитием АКК у взрослых [20,21].…”

Section: рисунокunclassified

Morphological diagnosis and assessment of the malignant potential in pediatric adrenal cortical neoplasms

Клецкая¹,

Рогожин²,

Коновалов³

et al. 2018

PHOI

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Adrenal cortical neoplasms in pediatric population are very rare. The clinical manifestations and biological behavior can be different from their counterparts in adults. This review contains data of epidemiology, clinical features, histology, biological behavior of adrenal cortical neoplasms in adults and children with particular attention to the approaches to malignancy assessment in different age groups.

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MicroRNA Era: The Importance for Diagnosis and Prognosis of Adrenocortical Tumors

Cited by 7 publications

References 58 publications

RETRACTED: MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

RETRACTED: MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway

Morphological diagnosis and assessment of the malignant potential in pediatric adrenal cortical neoplasms

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