MicroRNA expression profiles of trophoblastic cells

Morales-Prieto, Diana M.; Chaiwangyen, Wittaya; Ospina‐Prieto, Stephanie; Schneider, Ursula; Herrmann, Jörg; Gruhn, Bernd; Markert, Udo R.

doi:10.1016/j.placenta.2012.05.009

Cited by 225 publications

(219 citation statements)

References 41 publications

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“…Gene expression profiling has demonstrated that choriocarcinoma and SV40 transfected trophoblasts differed considerably both from each other and from primary cytotrophoblast and extravillous trophoblast [41]. Additionally, miRNA fingerprints of trophoblast cell lines and primary first and term trophoblasts have been found dissimilar [42], supporting our results. However, there are many examples of functional agreement between primary trophoblasts and derivative cell lines, supporting the use of these cell models [15,43].…”

Section: Multivariate Analysis Of Tlr Gene Expression and Cytokine Resupporting

confidence: 87%

“…The placenta contains different trophoblast types with specialized functions [3], and trophoblast cell lines can only partly reflect this complexity. Functional and phenotypic differences between trophoblast cell lines and primary trophoblasts have been extensively studied [38][39][40][41][42][43].…”

Section: Multivariate Analysis Of Tlr Gene Expression and Cytokine Rementioning

confidence: 99%

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Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

et al. 2015

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Section: Multivariate Analysis Of Tlr Gene Expression and Cytokine Resupporting

confidence: 87%

Section: Multivariate Analysis Of Tlr Gene Expression and Cytokine Rementioning

confidence: 99%

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

et al. 2015

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“…107 There are many miRNAs that are differentially expressed between the first and third trimesters of human pregnancy as demonstrated by in vitro experiments on first and third trimester cell lines. 9 Additionally, certain families of miRNAs are clustered together in chromosomal regions. As a result, although many of the miRNAs found to be differentially expressed in the placenta have different targets, they may be controlled by the same promoters and work in a synergistic manner.…”

Section: Microrna Regulation In the Placentamentioning

confidence: 99%

“…However, the HTR8/SVneo first trimester trophoblast cell line did not express these miRNAs. 9 From this group of miRNAs, miR-519-3P was recently shown to target Calcineurin subunit B type 1 (PPP3R1) and to encode a regulatory subunit of calcineurin in the human trophoblast. 110 These studies illustrate the importance of the C19MC miRNA cluster in human placenta.…”

Section: Microrna Regulation In the Placentamentioning

confidence: 99%

“…Since their discovery, miRNAs have emerged as major bioregulatory molecules of various processes including pregnancy. 6,[9][10][11] Pregnancy is a complex physiological process, and we are just beginning to understand the function of miRNAs during pregnancy. Successful mammalian pregnancy depends upon the establishment and maintenance of an adequate maternalfetal interface, and maternal immune tolerance of the semiallogeneic fetus.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNAs, immune cells and pregnancy

et al. 2014

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MicroRNAs (miRNAs) are a recently discovered class of non-coding RNAs that are expressed in many cell types, where they regulate the expression of complementary RNAs, thus modulating the stability and translation of mRNAs. miRNAs are predicted to regulate the expression of ,50% of all protein coding genes in mammals. Therefore, they participate in virtually all cellular processes investigated so far. Altered miRNAs expressions are associated with both physiological (pregnancy) and pathological processes (cancer). As the dynamic maternal-fetal interface plays a critical role in the maintenance of successful pregnancy, it is not surprising that the miRNAs that are unique to reproductive tissues are abundantly expressed. Research in this field has demonstrated the presence and dysregulation of a distinct set of pregnancy-associated miRNAs; however, most studies have centered on localizing various miRNAs in reproductive microdomains associated with normal or complicated pregnancies. Although several independent miRNA regulatory mechanisms associated with endometrial receptivity, immune cells, angiogenesis and placental development have been studied, miRNA-mediated regulation of pregnancy remains poorly understood. This review provides a summary of the current data on miRNA regulation as well as functional profiles of miRNAs that are found in the uterus, in immune cells associated with maternal tolerance to the fetus, and those involved in angiogenesis and placental development.

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miR‐346 and miR‐582‐3p‐regulated EG‐VEGF expression and trophoblast invasion via matrix metalloproteinases 2 and 9

Tsai

et al. 2016

BioFactors

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Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) is an important regulator for embryo implantation and placental development, and is clinically associated with several obstetric disorders related to insufficient or inappropriate trophoblast invasion, such as recurrent abortion, preeclampsia, and intrauterine fetal growth restriction. This study was performed to identify the microRNAs targeting EG-VEGF, and evaluate the regulatory effect on trophoblast biology. miR-346 and miR-582-3p were initially identified via bioinformatic tools, and their specific binding sites on the EG-VEGF 3'UTR were further confirmed using dual luciferase and a co-transfection assays. miR-346 and miR-582-3p were demonstrated not only to suppress EG-VEGF expression, but also inhibit trophoblast invasion and migration in the JAR and HTR-8/SVneo cell lines. We further evaluated the effect of microRNAs in HTR-8/SVneo cells coexpressing EG-VEGF and miR-346 or miR-582-3p on matrix metalloproteinase (MMP 2 and MMP 9) and the tissue inhibitors of metalloproteinase (TIMP 1 and TIMP 2) using RT-PCR, western blotting and gelatin zymography. TIMP 1 and TIMP 2 were not affected by the two microRNAs, whereas the expressions and activities of MMP 2 and MMP 9 were significantly downregulated, which in turn inhibited the invasion ability of trophoblasts. In conclusion, miR-346 and miR-582-3p regulate EG-VEGF-induced trophoblast invasion through repressing MMP 2 and MMP 9, and may become novel diagnostic biomarkers or therapeutic targets for EG-VEGF-related obstetric disorders. © 2016 BioFactors, 43(2):210-219, 2017.

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MicroRNA expression profiles of trophoblastic cells

Cited by 225 publications

References 41 publications

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

MicroRNAs, immune cells and pregnancy

miR‐346 and miR‐582‐3p‐regulated EG‐VEGF expression and trophoblast invasion via matrix metalloproteinases 2 and 9

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