MicroRNA miR-145-5p inhibits Phospholipase D 5 (PLD5) to downregulate cell proliferation and metastasis to mitigate prostate cancer

Liu, Juanni; Li, Junhai; Ma, Yongtu; Xu, Chunbao; Wang, Yigang; He, Yanfeng

doi:10.1080/21655979.2021.1945361

Cited by 25 publications

(13 citation statements)

References 37 publications

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“…Therefore, we performed a ceRNA analysis using the TCGA data and found that RP11-354B3.1 regulated MAPK4 expression by acting as a ceRNA for miR-145-5p in GC, which was further confirmed by luciferase report assay. miR-145 has been a well-known tumor suppressive microRNA in diverse types of cancer, including prostate cancer (Liu et al, 2021), hepatocellular carcinoma (Wang et al, 2021) and esophageal carcinoma (Fan et al, 2021). Our findings also confirmed that up-regulation of miR-145-5p could inhibit GC cells proliferation and decrease MAPK4 protein expression, which is consistent with the phenotype induced by down-regulation of RP11-354B3.1.…”

Section: Discussionsupporting

confidence: 82%

Comprehensive Genomic Characterization Analysis Identifies an Oncogenic Pseudogene RP11-3543B.1 in Human Gastric Cancer

Chen

et al. 2021

Front. Cell Dev. Biol.

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Background: Gastrointestinal Cancer (GICs) is the most common group of malignancies, and many of its types are the leading causes of cancer related death worldwide. Pseudogenes have been revealed to have critical regulatory roles in human cancers. The objective of this study is to comprehensive characterize the pseudogenes expression profiling and identify key pseudogenes in the development of gastric cancer (GC).Methods: The pseudogenes expression profiling was analyzed in six types of GICs cancer from The Cancer Genome Atlas RNA-seq data to identify GICs cancer related pseudogenes. Meanwhile, the genomic characterization including somatic alterations of pseudogenes was analyzed. Then, CCK8 and colony formation assays were performed to evaluate the biological function of RP11-3543B.1 and miR-145 in gastric cancer cells. The mechanisms of pseudogene RP11-3543B.1 in GC cells were explored via using bioinformatics analysis, next generation sequencing and lucifarese reporter assay.Results: We identified a great number of pseudogenes with significantly altered expression in GICs, and some of these pseudogenes expressed differently among the six cancer types. The amplification or deletion in the pseudogenes-containing loci involved in the alterations of pseudogenes expression in GICs. Among these altered pseudogenes, RP11-3543B.1 is significantly upregulated in gastric cancer. Down-regulation of RP11-3543B.1 expression impaired GC cells proliferation both in vitro and in vivo. RP11-3543B.1 exerts oncogene function via targeting miR-145-5p to regulate MAPK4 expression in gastric cancer cells.Conclusion: Our study reveals the potential of pseudogenes expression as a new paradigm for investigating GI cancer tumorigenesis and discovering prognostic biomarkers for patients.

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Section: Discussionsupporting

confidence: 82%

Comprehensive Genomic Characterization Analysis Identifies an Oncogenic Pseudogene RP11-3543B.1 in Human Gastric Cancer

Chen

et al. 2021

Front. Cell Dev. Biol.

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“…MiR-145, an anti-tumor microRNA [ 38 , 39 ], was validated to participate in multiple BC biological behaviors [ 40 , 41 ]. Jiang et al found that miR-145 was a negative correction with HBXIP in BC tissues and it suppressed BC cell proliferation via targeting HBXIP [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of microRNA-145 enhanced docetaxel sensitivity in breast cancer cells via inactivation of protein kinase B gamma-mediated phosphoinositide 3-kinase -protein kinase B pathway

Zhou¹,

Cai²,

Lu³

2022

Bioengineered

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Chemoresistance is a major challenge for the treatment of breast cancer (BC). Previous studies showed that miR-145 level decreases in chemoresistant BC tissues. Nevertheless, the biological function of miR-145 on docetaxel resistance of BC cells remains unclear, which is what our research attempted to clarify. RT-qPCR analyzed miR-145 level, and cell viability and colony formation assays assessed the impact of miR-145 on docetaxel resistance. Molecular mechanisms of miR-145-mediated docetaxel sensitivity were examined by Luciferase reporter assay and Western Blot assessed the function of AKT3 and PI3K/AKT signaling. Our research found that miR-145 expression presented significant downregulation in docetaxel-resistant BC cells. Meanwhile, miR-145 overexpression facilitated the docetaxel sensitivity of BC cells in vivo and in vitro , while the miR-145 inhibitor decreased the sensitivity of BC cells to docetaxel. We also observed that miR-145 inhibited docetaxel resistance mainly via downregulation of the AKT3 expression and further inhibited PI3K/AKT pathway. To conclude, this research provides a novel strategy for improving chemosensitivity through the newly identified miR-145-AKT3/PI3K-AKT signaling pathway in BC.

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“…MiRNAs are known for mediating their target genes’ post-transcriptional silencing by targeting the 3ʹ-UTR of corresponding mRNA [ 35 ]. For instance, miR-145-5p could inhibit PLD5 resulting in downregulation of cell proliferation and metastasis in prostate cancer [ 36 ]. miR-1180-3p has been reported involving in the regulation of C11of54 in hepatocellular carcinoma and ST3GAL4 in cutaneous melanoma [ 30 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of microRNA-1180-3p for colorectal cancer and effect of its alteration on cell function

Zhang

Tian

2021

Bioengineered

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An early diagnosis and effective prognostic factors would greatly reduce the mortality rate of colorectal cancer (CRC). This research is intended to complete the evaluation of the prognostic value and potential role of miR-1180-3p in CRC. The miR-1180-3p levels were reduced in CRC patients’ tissues, blood, and human CRC cell lines. The ability of miR-1180-3p was explored in discrimination of CRC patients and healths and the value in overall survival estimate. The effect of miR-1180-3p dysregulation on the CRC cellular function was investigated. miR-1180-3p is downregulated in CRC tissues, blood and cells than normal ones. This lower expression was correlated with vascular invasion, lymph node metastasis, and TNM stage. With the use of ROC curve, miR-1180-3p showed discriminating ability in CRC patients and healthy subjects. With the result of Kaplan–Meier analysis and multi-multivariate Cox analysis, miR-1180-3p was an independent predictor for CRC patients’ overall survival. Utilizing CCK-8, Transwell and matrigel assays, overexpression of miR-1180-3p reduced cancer cell proliferation and mobility, but induced apoptosis, by targeting COL12A1 . miR-1180-3p might function as a suppressor in CRC progression and allowed the discovery of a new biomarker for diagnosis, prognosis and therapy target for CRC.

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MicroRNA miR-145-5p inhibits Phospholipase D 5 (PLD5) to downregulate cell proliferation and metastasis to mitigate prostate cancer

Cited by 25 publications

References 37 publications

Comprehensive Genomic Characterization Analysis Identifies an Oncogenic Pseudogene RP11-3543B.1 in Human Gastric Cancer

Comprehensive Genomic Characterization Analysis Identifies an Oncogenic Pseudogene RP11-3543B.1 in Human Gastric Cancer

Overexpression of microRNA-145 enhanced docetaxel sensitivity in breast cancer cells via inactivation of protein kinase B gamma-mediated phosphoinositide 3-kinase -protein kinase B pathway

Clinical significance of microRNA-1180-3p for colorectal cancer and effect of its alteration on cell function

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