MicroRNA profiles of BMSCs induced into osteoblasts with osteoinductive medium

Yan, Zhixiong; Guo, Yong; Wang, Yang; Li, Yanan; Wang, Jiahui

doi:10.3892/etm.2018.5723

Cited by 9 publications

(10 citation statements)

References 40 publications

(31 reference statements)

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“…MicroRNAs including miR-31, miR-138, miR-204, and mir-637 have been investigated to inhibit osteogenic differentiation of BMSCs [105][106][107][108]. However, recently, Yan et al reported that let-7c-5p, miR-181c-3p, miR-3092-3p and miR-5132-3p promoted osteogenic differentiation of mouse BMSCs [109].…”

Section: Epigenetic Factors Involved In Osteogenic Differentiation Ofmentioning

confidence: 99%

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Qadir

Liang

et al. 2020

IJMS

168

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Senile osteoporosis has become a worldwide bone disease with the aging of the world population. It increases the risk of bone fracture and seriously affects human health. Unlike postmenopausal osteoporosis which is linked to menopause in women, senile osteoporosis is due to aging, hence, affecting both men and women. It is commonly found in people with more than their 70s. Evidence has shown that with age increase, bone marrow stromal cells (BMSCs) differentiate into more adipocytes rather than osteoblasts and undergo senescence, which leads to decreased bone formation and contributes to senile osteoporosis. Therefore, it is necessary to uncover the molecular mechanisms underlying the functional changes of BMSCs. It will benefit not only for understanding the senile osteoporosis development, but also for finding new therapies to treat senile osteoporosis. Here, we review the recent advances of the functional alterations of BMSCs and the related mechanisms during senile osteoporosis development. Moreover, the treatment of senile osteoporosis by aiming at BMSCs is introduced.

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Section: Epigenetic Factors Involved In Osteogenic Differentiation Ofmentioning

confidence: 99%

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Qadir

Liang

et al. 2020

IJMS

168

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“…BMSC osteogenesis is the key step in bone regeneration and is affected by several factors including hormones, growth factors, environmental factors, and microRNAs [11]. BMSCs not only give rise to bone tissues but can also differentiate into adipose cells or osteoblasts [60].…”

Section: Role Of Bmscs In Bone Regenerative Therapymentioning

confidence: 99%

“…Therefore, the current focus of treating bone disorders is tissue regeneration using bone marrow mesenchymal stem cells (BMSCs) [7, 8]. A number of studies have investigated the effects of various drugs, mechanical stress, physical stimuli, and scaffolds on BMSCs [9–11], in order to clinically translate its regenerative capacity [12]. Traditional Chinese medicine (TCM) has also garnered considerable interest in recent years due to its minimal toxicity [13].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Action of Icariin in Bone Marrow Mesenchymal Stem Cells

Yang

et al. 2019

Stem Cells International

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Osteoporosis, femoral head necrosis, and congenital bone defects are orthopedic disorders characterized by reduced bone generation and insufficient bone mass. Bone regenerative therapy primarily relies on the bone marrow mesenchymal stem cells (BMSCs) and their ability to differentiate osteogenically. Icariin (ICA) is the active ingredient of Herba epimedii, a common herb used in traditional Chinese medicine (TCM) formulations, and can effectively enhance BMSC proliferation and osteogenesis. However, the underlying mechanism of ICA action in BMSCs is not completely clear. In this review, we provide an overview of the studies on the role and mechanism of action of ICA in BMSCs, to provide greater insights into its potential clinical use in bone regeneration.

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“…In support of our results, four of the six miRNAs ( miR-449a , miR-378a , miR-344 , and miR-874 ) have already been implicated in regulating insulin signaling, improving metabolic dysregulation, and activating insulin synthesis[ 35 - 38 ]. The other two miRNAs ( miR-5132-3p and miR-3075) have been suggested to play a regulatory role in the proliferation and migration of osteoblasts and Schwann cells[ 39 , 40 ]. Hence, we propose that miR-5132-3p and miR-3075 may act as new effector molecules in β-cell regulation.…”

Section: Discussionmentioning

confidence: 99%

Analysis of long noncoding RNA-associated competing endogenous RNA network in glucagon-like peptide-1 receptor agonist-mediated protection in β cells

Bai¹,

Zhi²,

Liu³

et al. 2020

WJD

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BACKGROUND Long noncoding RNAs (lncRNAs) and mRNAs are widely involved in various physiological and pathological processes. The use of glucagon-like peptide-1 receptor agonists (GLP-1RAs) is a novel therapeutic strategy that could promote insulin secretion and decrease the rate of β-cell apoptosis in type 2 diabetes mellitus (T2DM) patients. However, the specific lncRNAs and mRNAs and their functions in these processes have not been fully identified and elucidated. AIM To identify the lncRNAs and mRNAs that are involved in the protective effect of GLP-1RA in β cells, and their roles. METHODS Rat gene microarray was used to screen differentially expressed (DE) lncRNAs and mRNAs in β cells treated with geniposide, a GLP-1RA. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to assess the underlying functions of DE mRNAs. Hub mRNAs were filtered using the STRING database and the Cytoscape plugin, CytoHubba. In order to reveal the regulatory relationship between lncRNAs and hub mRNAs, their co-expression network was constructed based on the Pearson coefficient of DE lncRNAs and mRNAs, and competing endogenous RNA (ceRNA) mechanism was explored through miRanda and TargetScan databases. RESULTS We identified 308 DE lncRNAs and 128 DE mRNAs with a fold change filter of ≥ 1.5 and P value < 0.05. GO and KEGG pathway enrichment analyses indicated that the most enriched terms were G-protein coupled receptor signaling pathway, inflammatory response, calcium signaling pathway, positive regulation of cell proliferation, and ERK1 and ERK2 cascade. Pomc , Htr2a , and Agtr1a were screened as hub mRNAs using the STRING database and the Cytoscape plugin, CytoHubba. This result was further verified using SwissTargetPrediction tool. Through the co-expression network and competing endogenous (ceRNA) mechanism, we identified seven lncRNAs (NONRATT027738, NONRATT027888, NONRATT030038, etc .) co-expressed with the three hub mRNAs ( Pomc, Htr2a, and Agtr1a ) based on the Pearson coefficient of the expression levels. These lncRNAs regulated hub mRNA functions by competing with six miRNAs ( r no-miR-5132-3p, rno-miR-344g, rno-miR-3075, etc .) via the ceRNA mechanism. Further analysis indicated that lncRNA NONRATT027738 interacts with all the three hub mRNAs, suggesting that it is at a core position within the ceRNA network. CONCLUSION We have identified key lncRNAs and mRNAs, and highlighted here how they interact through the ceRNA mechanism to mediate the protective effect of GLP-1RA in β cells.

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MicroRNA profiles of BMSCs induced into osteoblasts with osteoinductive medium

Cited by 9 publications

References 40 publications

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Mechanism of Action of Icariin in Bone Marrow Mesenchymal Stem Cells

Analysis of long noncoding RNA-associated competing endogenous RNA network in glucagon-like peptide-1 receptor agonist-mediated protection in β cells

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