MicroRNA profiling of rats with ochratoxin A nephrotoxicity

Abstract

BackgroundNephrotoxicity is the most prominent one among the various toxicities of ochratoxin A (OTA). MicroRNAs (miRNAs) are small non-coding RNAs that have an impact on a wide range of biological processes by regulating gene expression at post-transcriptional level or protein systhesis level. The objective of this study is to analyze miRNA profiling in the kidneys of rats gavaged with OTA.ResultsTo profile miRNAs in the kidneys of rats with OTA nephrotoxicity, high-throughput sequencing and bioinformatics approaches were applied to analyze the miRNAs in the kidney of rats following OTA treatment. A total of 409 known miRNAs and 8 novel miRNAs were identified in the kidney and the levels of the novel miRNAs were varied in response to different doses of OTA. Expression of miR-129, miR-130a, miR-130b, miR-141, miR-218b and miR-3588 were uniquely suppressed in mid dose but then elevated in high dose, with opposite expression to their target genes. The expression pattern was closely related with the “MAPK signaling pathway”. Dicer1 and Drosha were significantly suppressed, indicating an impairment of miRNA biogenesis in response to OTA.ConclusionsThe abrogation of miRNA maturation process suggests a new target of OTA toxicity. Moreover, the identification of the differentially expressed miRNAs provides us a molecular insight into the nephrtoxicity of OTA.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-333) contains supplementary material, which is available to authorized users.

Ochratoxin A induced early hepatotoxicity: new mechanistic insights from microRNA, mRNA and proteomic profiling studies

“…In contrast, ALB activity of c group was slightly higher in high-dose group than in control rats 13 . The liver damage in highdose group is more serious than that in medial dose group at 13 weeks.…”

“…After the administration of OTA, body mass of the high-dose rats reduced about 7% (p , 0.05) at 9 and 10 weeks 13 , but gradually recovered thereafter. The liver weights were comparable between OTA-treated and control rats at 2, 4 and 13 weeks (Fig.…”

“…The targets predicted by at least two different programs were chosen -as many investigators have recently been utilized this ''at least 2 algorithms'' approach to effectively reduce false positive targets 13,[17][18][19][20][21][22] . In a next step, further analysis was carried out on these selected putative target genes.…”

“…After the gavage, rats were anesthetized using chloral hydrate (6%, 5 ml/kg bw, ip). Blood samples were collected from the inner canthus before decapitation for determination of blood parameters including alanine (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), albumin (ALB), creatinine (CREA), glucose (GLU), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) using a Hitachi 7020 automatic biochemical analyzer (Hitachi, Tokyo, Japan) 13 .…”

Exposure to ochratoxin A through consumption of grape juices produced by steam distillation method and intended for school meals

“…Exposure to OTA through food consumption poses a health risk, since this mycotoxin has been related to nephrotoxicity (DAI et al, 2014). The International Agency for Research on Cancer (IARC) classified OTA as possible carcinogen to humans (IARC, 1993).…”