MicroRNAs aid the assessment of programmed death ligand�1 expression in patients with non‑small cell lung cancer

Grenda, Anna; Nicoś, Marcin; Szczyrek, Michał; Krawczyk, Paweł; Kucharczyk, Tomasz; Jarosz, Bożena; Pankowski, Juliusz; Sawicki, Marek; Szumiło, Justyna; Bukała, Paulina; Milanowski, Janusz

doi:10.3892/ol.2019.10207

Cited by 15 publications

(17 citation statements)

References 42 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These immunotherapies block the PD-1 checkpoint to enable the immune system to recognize and target cancer cells. There are now multiple antibodies that are raised against anti-PD1 or anti-PD-L1, including Atezolizumab, Avelumab, Durvalumab, Pembrolizumab, and Nivolumab (120).…”

Section: Immune-checkpoint Inhibitorsmentioning

confidence: 99%

The Therapeutic Potential of DNA Damage Repair Pathways and Genomic Stability in Lung Cancer

et al. 2020

View full text Add to dashboard Cite

Section: Immune-checkpoint Inhibitorsmentioning

confidence: 99%

The Therapeutic Potential of DNA Damage Repair Pathways and Genomic Stability in Lung Cancer

et al. 2020

View full text Add to dashboard Cite

“…In the last 10 years, miRNA have been demonstrated to participate actively in human tumorigenesis [ 35 – 38 ]. More specifically, studies have found miR-203 to possess tumor-suppressive function by regulating downstream protein-coding genes in gastric cancer, lung cancer, breast cancer and ESCC [ 39 – 42 ].…”

Section: Introductionmentioning

confidence: 99%

Circ0120816 acts as an oncogene of esophageal squamous cell carcinoma by inhibiting miR-1305 and releasing TXNRD1

Song

Wang

et al. 2020

Cancer Cell Int

View full text Add to dashboard Cite

Background Circular RNAs (circRNAs) have been discovered to participate in the carcinogenesis of multiple cancers. However, the role of circRNAs in esophageal squamous cell carcinoma (ESCC) progression is yet to be properly understood. This research aimed to investigate and understand the mechanism used by circRNAs to regulate ESCC progression. Methods Bioinformatics analysis was first performed to screen dysregulated circRNAs and differentially expressed genes in ESCC. The ESCC tissue samples and adjacent normal tissue samples utilized in this study were obtained from 36 ESCC patients. All the samples were subjected to qRT-PCR analysis to identify the expression of TXNRD1, circRNAs, and miR-1305. Luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay were later conducted to verify the existing relationship among circ0120816, miR-1305 and TXNRD1. CCK-8, BrdU, cell adhesion, cell cycle, western blot and caspase 3 activity assays were also employed to evaluate the regulation of these three biological molecules in ESCC carcinogenesis. To evaluate the effect of circ0120816 on ESCC tumor growth and metastasis, the xenograft mice model was constructed. Results Experimental investigations revealed that circ0120816 was the highest upregulated circRNA in ESCC tissues and that this non-coding RNA acted as a miR-1305 sponge in enhancing cell viability, cell proliferation, and cell adhesion as well as repressing cell apoptosis in ESCC cell lines. Moreover, miR-1305 was observed to exert a tumor-suppressive effect in ESCC cells by directly targeting and repressing TXNRD1. It was also noticed that TXNRD1 could regulate cyclin, cell adhesion molecule, and apoptosis-related proteins. Furthermore, silencing circ0120816 was found to repress ESCC tumor growth and metastasis in vivo. Conclusions This research confirmed that circ0120816 played an active role in promoting ESCC development by targeting miR-1305 and upregulating oncogene TXNRD1.

show abstract

“…In the past 10 years, miRNA had been demonstrated to take an essential part in tumorigenesis [27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1

Song

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The morbidity and mortality of esophageal squamous cell carcinoma (ESCC) remains stubbornly high, in spite of emerging new diagnoses methods. The role of circular RNAs (circRNAs) in ESCC progression is still in need of more exploration. Methods: In this research, we gathered 36 patients’ ESCC tissues to analyze the expression of circ0120816, miR-1305 and TXNRD1. KYSE450 and KYSE510 cells were used to conduct the transfection. Aiming to verify our hypothesis, qRT-PCR, RNase R treatment, nuclear extraction, luciferase reporter assay, RNA immunoprecipitation assay, CCK-8, BrdU, cell adhesion, caspase 3 activity assay were used. Results: Circ0120816, upregulated in ESCC, acted as a sponge for miR-1305. Circ0120816 combined miR-1305 to enhance cell viability, proliferation adhesion and repress cell apoptosis in ESCC cell lines. On the contrary, miR-1305 exerted reversed effect in ESCC cells through decreasing TXNRD1. Conclusions: This research demonstrated that circ0120816 promoted ESCC development by competitive binding miR-1305 which could increase the expression of TXNRD1.

show abstract

MicroRNAs aid the assessment of programmed death ligand�1 expression in patients with non‑small cell lung cancer

Cited by 15 publications

References 42 publications

The Therapeutic Potential of DNA Damage Repair Pathways and Genomic Stability in Lung Cancer

The Therapeutic Potential of DNA Damage Repair Pathways and Genomic Stability in Lung Cancer

Circ0120816 acts as an oncogene of esophageal squamous cell carcinoma by inhibiting miR-1305 and releasing TXNRD1

Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1

Contact Info

Product

Resources

About