MicroRNAs and cataracts: correlation among let-7 expression, age and the severity of lens opacity

Peng, Chi Hsien; Liu, Jorn Hon; Woung, Lin‐Chung; Lin, Tzu Jung; Chiou, Shih Hwa; Tseng, Po Chen; Du, Wei; Cheng, Cheng Kuo; Hu, Chao S.; Chien, Ke Hung; Chen, Shih‐Jen

doi:10.1136/bjophthalmol-2011-300585

Cited by 52 publications

(40 citation statements)

References 22 publications

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“…Hughes et al [15] found that mutation in the seed region of miR-184 caused anterior polar cataract. Peng et al [16] demonstrated that let-7 expression was significantly related to the severity of lens opacity and age of age-related cataract. Xu et al [33] found that at least 78 miRNAs were expressed in adult mouse retina, some of which were associated with retinal development, circadian rhythm (miR-183), retinal pigment degeneration (miR-1, miR-133, miR-183), and macular degeneration (miR-31, miR-150).…”

Section: Discussionmentioning

confidence: 98%

“…MiRNAs regulate mRNA degradation or translational interference by binding to a complementary sequence found in the 3′-untranslated regions (UTR) of target gene mRNAs [10]. In the past decade, more and more studies have demonstrated that aberrant expression of miRNAs was tightly linked to the pathogenesis of many age-related disease [11][12][13][14], including cataract progression [15,16]. MiRNA research on the treatment of diseases has also made new breakthroughs [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in age-related cataract

Zhao

Min

et al. 2014

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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MicroRNA-125b (miR-125b) has been implicated in a variety of diseases as either repressors or promoters, and plays crucial roles in many cellular processes such as cell differentiation, proliferation and apoptosis. Age-related cataract has become one of the most serious problems facing the aging population in the world. The purpose of this study was to investigate the role of miR-125b in the development of age-related cataract. We demonstrated that miR-125b was downregulated in both age-related cataract tissue and lens epithelial cell apoptosis induced by UV irradiation. We also identified the impact of miR-125b on apoptosis in a lens epithelial cell line. In vitro, miR-125b regulates human lens epithelial cell apoptosis at least in part by directly targeting p53. In addition,an inverse relationship between miR-125b and p53 expression was seen in age-related cataract tissue. In conclusion,this study suggests that miR-125b might be closely involved in the pathogenesis of cataract, and has the potential to be a diagnostic biomarker or even a therapeutic modality for cataract.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in age-related cataract

Zhao

Min

et al. 2014

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…Bioinformatics prediction reveals that lncRNA n371509 could act as a let-7 sponge and regulate the let-7 function for binding its target gene. Peng et al demonstrated that the let-7b microRNA expression was positively associated with patient age and severity of lens opacity [53]. The lncRNA XIST/let-7/TGF-β regulatory network might play a role in the pathogenesis of ARC [54][55][56].…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Liu

Shan

et al. 2018

Cell Physiol Biochem

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Background/Aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation. Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675. Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3’UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress. Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.

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“…Moreover HMGA2 expression is decreased in the lens of elderly humans through let7b microRNA regulation. The reduction ratio is correlated with the severity of lens opacity (Peng et al, 2012). In reported cases of HMGA2 deletion, eye phenotype was not described.…”

Section: Discussionmentioning

confidence: 95%

Complex translocation t(1;12;14)(q42;q14;q32) and HMGA2 deletion in a fetus presenting growth delay and bilateral cataracts

Raymond¹,

Francou

Petit³

et al. 2015

European Journal of Medical Genetics

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MicroRNAs and cataracts: correlation among let-7 expression, age and the severity of lens opacity

Abstract: These findings suggest that microRNAs play a role in age-related cataracts. A local let-7b microRNA increase may represent a risk factor in the formation of age-related cataracts.

Cited by 52 publications

References 22 publications

MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in age-related cataract

MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in age-related cataract

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Complex translocation t(1;12;14)(q42;q14;q32) and HMGA2 deletion in a fetus presenting growth delay and bilateral cataracts

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