MicroRNAs as potential biomarkers to predict the risk of urinary retention following intradetrusor onabotulinumtoxin‐A injection

Chermansky, Christopher; Kadow, Brian T.; Kashyap, Mahendra; Tyagi, Pradeep

doi:10.1002/nau.23296

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…Kashyap et al found that miR‐132 and miR‐221 were dysregulated in rat OAB models, and these miRNAs were effective in bladder hypertrophy and dysfunction. Chermansky et al also associated dysregulation of miR‐221 and miR‐125b with patients with OAB . Cordes et al reported that miR‐143 and miR‐145 regulate smooth muscle tone, and Imamura et al also stated that miR‐1 may be a new therapeutic target in OAB with the role of reducing the smooth muscle contraction of the bladder.…”

Section: Discussionmentioning

confidence: 99%

“…Chermansky et al 24 also associated dysregulation of miR-221 and miR-125b with patients with OAB. 24 Cordes et al 25 reported that miR-143 and miR-145 regulate smooth muscle tone, and Imamura et al 26 also stated that miR-1 may be a new therapeutic target in OAB with the role of reducing the smooth muscle contraction of the bladder. The current study also supported the view that dysregulation in expression levels of miRNAs was effective in regulating smooth muscle tone in the bladder.…”

Section: Discussionmentioning

confidence: 99%

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Exploring biomarkers in the overactive bladder: Alterations in miRNA levels of a panel of genes in patients with OAB

Fırat

Aybek

Akgün

et al. 2019

Neurourology and Urodynamics

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Aims It has been demonstrated that there are abundant stable microRNAs (miRNAs) in plasma, which is potentially disease‐specific. Adrenergic and muscarinic pathways play an important role in voiding physiology. Alterations in the levels of miRNAs are thought to influence the regulation of these pathways at the molecular level. The aim of this study was to investigate the relationship of miRNAs with overactive bladder pathogenesis and to provide a new perspective to treatment approaches. Methods This study included patients with an overactive bladder (OAB) diagnosis and a healthy control group. All patients completed a validated OAB‐V8 questionnaire. The relative expression levels of 12 miRNAs were examined in plasma by PCR. Receiver operating characteristic (ROC) curves were generated to evaluate the diagnostic qualification of miRNAs. Results The relative expression levels of let‐7b‐5p, miR‐92a‐3p, miR‐98‐5p, miR‐142‐3p, and miR‐200c‐3p were significantly upregulated and miR‐139‐5p was significantly downregulated in patients with OAB and no correlation was determined between the levels of miRNAs with OAB symptom score. Among the miRNAs, miR‐98‐5p provided the highest diagnostic accuracy alone (area under curve [AUC] = 0.79) in ROC analysis. The combination of miR‐98‐5p + miR‐139‐5p was seen to be a good indicator (AUC = 0.839). Conclusions These results suggest that alteration of the miRNA levels can be used as auxiliary parameters to explain the pathophysiology of OAB syndrome and could shed light on new treatment options.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploring biomarkers in the overactive bladder: Alterations in miRNA levels of a panel of genes in patients with OAB

Fırat

Aybek

Akgün

et al. 2019

Neurourology and Urodynamics

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“…39,40 Additionally, nonstandard testing, such as urethral function RNA testing, or nonurologic factors may also be useful to improve model accuracy. [41][42][43] Additionally, the performance of these models was not compared to clinical experts. Although prediction models are consistently superior to clinical judgment, this remains to be directly shown for these models, and this comparison is recommended in future studies.…”

Section: T a B L E 2 Coefficients For The Prediction Modelsmentioning

confidence: 99%

Predicting outcomes after intradetrusor onabotulinumtoxina for non‐neurogenic urgency incontinence in women

Hendrickson

Xie

Rahn

et al. 2021

Neurourology and Urodynamics

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Aims: Develop models to predict outcomes after intradetrusor injection of 100 or 200 units of onabotulinumtoxinA in women with non-neurogenic urgency urinary incontinence (UUI).Methods: Models were developed using 307 women from two randomized trials assessing efficacy of onabotulinumtoxinA for non-neurogenic UUI. Cox, linear and logistic regression models were fit using: (1) time to recurrence over 12 months, (2) change from baseline daily UUI episodes (UUIE) at 6 months, and (3) need for self-catheterization over 6 months. Model discrimination of Cox and logistic regression models was calculated using c-index. Mean absolute error determined accuracy of the linear model. Calibration was demonstrated using calibration curves. All models were internally validated using bootstrapping.Results: Median time to recurrence was 6 (interquartile range [IQR]: 2-12) months. Increasing age, 200 units of onabotulinumtoxinA, higher body mass index (BMI) and baseline UUIE were associated with decreased time to recurrence. The c-index was 0.63 (95% confidence interval [CI]: 0.59, 0.67). Median change in daily UUIE from baseline at 6 months was −3.5 (IQR: −5.0, −2.3). Increasing age, lower baseline UUIE, 200 units of onabotulinumtoxinA, higher BMI and IIQ-SF were associated with less improvement in UUIE. The mean absolute error predicting change in UUIE was accurate to 1.6 (95% CI:1.5, 1.7) UUI episodes. The overall rate of self-catheterization was 17.6% (95% CI: 13.6%-22.4%). Lower BMI, 200 units of onabotulinumtoxinA, increased baseline postvoid residual and maximum capacity were associated with higher risk of self-catheterization. The c-index was 0.66 (95% CI: 0.61, 0.76). The three calculators are available at http://riskcalc.duke.edu.

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“…MiRs are non-coding RNAs, approximately 19-23 nucleotides (nt) in length. They participate in epigenetic post-transcriptional control of protein-coding gene expression primarily by reversible translational repression or mRNA destabilization/ degradation and thereby inhibit protein synthesis (6).…”

Section: Original Articlementioning

confidence: 99%

“…Recently, Chermansky et al reported that the elevated expression of miR-221 and miR-125b in detrusor overactivity in the bladder tissue of patients may predict their high risk for undergoing urinary retention following intradetrusor injection of onabotulinumtoxin-A (6). In addition, the combination of upregulated miR-98-5p and downregulated miR-139-5p in the plasm of patients' overactive bladder (OAB) was found to be a useful biomarker for OAB.…”

Section: Original Articlementioning

confidence: 99%

MicroRNA expression profile in the spinal cord injured rat neurogenic bladder by next-generation sequencing

Shang¹,

Ou²,

Xu³

et al. 2020

Transl Androl Urol

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Background: An increasing amount of evidence has indicated that microRNAs (miRs) are involved in most biological conditions, including the neurogenic bladder (NB). However, to our knowledge, no studies have investigated these miR expressions in spinal cord-injured (SCI) rat NB. The goal of the study was to explore the miR expression profile in the SCI rat NB by next-generation sequencing (NGS).Methods: Female Wistar rats underwent spinal cord transection at T9-10 and were randomly divided into the SCI-1, SCI-2 and SCI-3 groups (n=5 for each group) whose bladder tissues were collected 1, 2, and 4 weeks after transection, respectively. The normal rats were used as the normal control (NC) group.MiRs microarray assays were used to detect the differentially expressed miRs between the groups by NGS, which was then verified by quantitative real-time polymerase chain reaction (qRT-PCR). Those significantly differently expressed miRs were analyzed with Gene Ontology categories and Kyoto Encyclopedia of Genes and Genomes bioinformatical analyses.Results: Compared with the NC group, 96, 28 and 51 miRs were downregulated in the rats' bladder in the SCI-1, SCI-2, and SCI-3 groups, respectively, and 133, 49, and 76 miRs were upregulated respectively. Specifically, miR-21-5p was the most significantly upregulated miR in all SCI groups. Also, 121 miRs (SCI-1 vs. SCI-2), 98 miRs (SCI-1 vs. SCI-3), and 26 miRs (SCI-2 vs. SCI-3) were of significantly different expression. Furthermore, a large set of genes implicated in essential signaling pathways were targeted by these miRs, including PI3K-Akt, MAPK, Rap1, and cGMP-PKG signaling pathways, along with the tight junction and metabolic pathways.Conclusions: This is the first demonstration of differentially expressed miRs, which may potentially serve as new molecular targets in the SCI rat NB.

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MicroRNAs as potential biomarkers to predict the risk of urinary retention following intradetrusor onabotulinumtoxin‐A injection

Cited by 8 publications

References 26 publications

Exploring biomarkers in the overactive bladder: Alterations in miRNA levels of a panel of genes in patients with OAB

Exploring biomarkers in the overactive bladder: Alterations in miRNA levels of a panel of genes in patients with OAB

Predicting outcomes after intradetrusor onabotulinumtoxina for non‐neurogenic urgency incontinence in women

MicroRNA expression profile in the spinal cord injured rat neurogenic bladder by next-generation sequencing

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