MicroRNAs in nasopharyngeal carcinoma

Spence, Tara; Bruce, Jeff; Yip, Kenneth W.; Liu, Fei‐Fei

doi:10.21037/cco.2016.03.09

Cited by 50 publications

(62 citation statements)

References 70 publications

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“…Numerous studies have functionally characterized causal links between miRNAs and tumour development or progression in a subset of non-HPV related HNC (reviewed in [56]), which further highlights the utility of miRNAs as accurate tumour biomarkers. However, miRNA signatures have not been incorporated into clinical practice to date.…”

Section: Determination Of Hpv Status and Hpv As A Biomarkermentioning

confidence: 99%

HPV Associated Head and Neck Cancer

Spence

Bruce

Yip

et al. 2016

Cancers

Self Cite

114

108

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Head and neck cancers (HNCs) are a highly heterogeneous group of tumours that are associated with diverse clinical outcomes. Recent evidence has demonstrated that human papillomavirus (HPV) is involved in up to 25% of HNCs; particularly in the oropharyngeal carcinoma (OPC) subtype where it can account for up to 60% of such cases. HPVs are double-stranded DNA viruses that infect epithelial cells; numerous HPV subtypes, including 16, 18, 31, 33, and 35, drive epithelial cell transformation and tumourigenesis. HPV positive (HPV+) HNC represents a distinct molecular and clinical entity from HPV negative (HPV−) disease; the biological basis for which remains to be fully elucidated. HPV positivity is strongly correlated with a significantly superior outcome; indicating that such tumours should have a distinct management approach. This review focuses on the recent scientific and clinical investigation of HPV+ HNC. In particular, we discuss the importance of molecular and clinical evidence for defining the role of HPV in HNC, and the clinical impact of HPV status as a biomarker for HNC.

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Section: Determination Of Hpv Status and Hpv As A Biomarkermentioning

confidence: 99%

HPV Associated Head and Neck Cancer

Spence

Bruce

Yip

et al. 2016

Cancers

Self Cite

114

108

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“…As NPC is highly correlated to the Epstein-Barr virus (EBV) infection, EBV viral RNAs (miRNAs56, long non-coding RNAs7) and proteins are of diagnostic powers. In clinical practice, EBV capsid antigen IgA (VCA-IgA), early antigen Ig A (EA-IgA), nuclear antigen (EBNA1) IgA, and circulating EBV DNA are widely used for diagnosis and screening of NPC8910.…”

mentioning

confidence: 99%

SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma

Luo

Mok

Lin

et al. 2017

Sci Rep

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Nasopharyngeal carcinoma (NPC) is a serious threat to public health, and the biomarker discovery is of urgent needs. The data-independent mode (DIA) based sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS) has been proved to be precise in protein quantitation and efficient for cancer biomarker researches. In this study, we performed the first SWATH-MS analysis comparing the NPC and normal tissues. Spike-in stable isotope labeling by amino acids in cell culture (super-SILAC) MS was used as a shotgun reference. We identified and quantified 1414 proteins across all SWATH-MS analyses. We found that SWATH-MS had a unique feature to preferentially detect proteins with smaller molecular weights than either super-SILAC MS or human proteome background. With SWATH-MS, 29 significant differentially express proteins (DEPs) were identified. Among them, carbonic anhydrase 2 (CA2) was selected for further validation per novelty, MS quality and other supporting rationale. With the tissue microarray analysis, we found that CA2 had an AUC of 0.94 in differentiating NPC from normal tissue samples. In conclusion, SWATH-MS has unique features in proteome analysis, and it leads to the identification of CA2 as a potentially new diagnostic biomarker for NPC.

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“…Many researchers are confronted with difficulties in looking for biomarkers for NPC, which is useful to study cellular processes and monitor or recognize disruption or alterations in the cellular processes of cancer cells. 29,30 These studies can reveal why NPC occurs, and they provide specific biological targets for the early diagnosis of NPC. Biomarkers in relation to nanotechnology is a new era of early cancer diagnosis and precise drug delivery.…”

Section: Discussionmentioning

confidence: 99%