microRNAs in the blastocoel fluid as accessible indicators of chromosomal normality

Esmaeilivand, Masoumeh; Fattahi, Amir; Abedelahi, Ali; Hamdi, Kobra; Farzadi, Laya; Goharitaban, Sepide; Niknafs, Behrooz

doi:10.1016/j.repbio.2022.100695

Cited by 4 publications

(6 citation statements)

References 25 publications

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“…In a recent study, Esmaeilivand et al explored the expression levels of predefined miRNAs associated with aneuploidy and implantation failure, including miR-345, miR-339, miR-141, miR-27b, miR-191, miR-20a, miR-30c, miR-661, miR-372, and miR-142 ( 16 ). Assessing blastocoel samples obtained from euploid and aneuploid blastocysts, the results revealed a significant increase of only miR-20a and miR-661 in the blastocoel fluid obtained from aneuploid embryos.…”

Section: Resultsmentioning

confidence: 99%

“…The role of miRNA in early development has been previously investigated, showing significant contribution in the process of implantation and stem cell differentiation (8-10). However, only limited studies have explored the potential of miRNAs in assessing developmental competence of early embryos, and the possible relationship between aneuploidy and miRNA expression (11)(12)(13)(14)(15)(16)(17)(18). In this systematic review, our aim is to identify and combine the reported information on miRNAs with potential association to aneuploidy in preimplantation blastocysts.…”

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confidence: 99%

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Differentially expressed microRNAs in aneuploid preimplantation blastocysts: a systematic review

Almutlaq,

Viñals Gonzalez,

SenGupta

2024

Front. Reprod. Health

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IntroductionMicroRNAs are small noncoding genes with gene expression regulatory function. Their emergence as potential diagnostic biomarker for many diseases has gained a specific interest among researchers. Observations of changes in miRNA levels correlating with aneuploidy in early embryos raise the prospective of employing miRNA as biomarkers to assess the embryo quality.MethodTo identify and gather the miRNAs with potential link to chromosomal abnormalities in embryos from previous research, we conducted a systematic search using four databases, including Embase, Medline, Web of Science, and Cochrane databases in accordance with PRISMA guidelines.ResultsOut of 200 identified records, only seven met the inclusion criteria. Seven miRNAs: miR-19b, miR-517c, miR-518e, miR-522, miR-92a, and miR-106a exhibited persistent downregulation in aneuploid blastocysts in the included studies. These miRNAs are members of important miRNA clusters, associated with abnormal expression in studies on reproductive failure. Pathway analysis revealed their involvement in regulating gene transcription, as well as cell cycle progression and apoptosis.DiscussionThe changes detected in the miRNA expression in aneuploid embryos across different studies support the aneuploidy and miRNA relationship and prospect miRNA as a valuable tool for the assessment of embryo quality. Collectively, these observations highlight the role of miRNAs in embryonic development, and their involvement in genetic abnormalities that occur in embryos, such as aneuploidy, indicating their potential implementation to improve the embryo selection and reproductive outcomes.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Differentially expressed microRNAs in aneuploid preimplantation blastocysts: a systematic review

Almutlaq,

Viñals Gonzalez,

SenGupta

2024

Front. Reprod. Health

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“…The procedures for investigations involving human subject samples were approved by the ethics council of Tabriz University of Medical Sciences in Iran (IR.TBZMED.REC.1398.1219), and written informed consent was obtained from all couples. Embryos from a previously published study conducted by our group were utilized [10]. On the third day of development, laser ablation (Hamilton Thorne Biosciences, Inc., Beverly, MA) was used to biopsy one cell from each embryo.…”

Section: Methodsmentioning

confidence: 99%

“…The primer pair sequences can be found in a previous study conducted by our group [10]. To evaluate miRNA levels, we run 45 cycles, with each cycle including primary denaturation for 10 seconds at 95°C, annealing temperature speci c for each primer for 10 seconds, and extension at 60°C for 60 seconds.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate miRNA levels, we run 45 cycles, with each cycle including primary denaturation for 10 seconds at 95°C, annealing temperature speci c for each primer for 10 seconds, and extension at 60°C for 60 seconds. The primer pair sequences can also be found in a previous study conducted by our group [10]. Each assay was performed in triplicate, and the gene expression was calculated based on the 2 −ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

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Association of Trophectoderm mRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Esmaeilivand,

Ghasemzadeh,

Niknafs

et al. 2023

Reprod. Sci.

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MicroRNAs (miRNAs) and mRNAs can serve as indicators of the chromosomal state of an embryo, with different pro les observed in euploid and aneuploid blastocysts. Examining the levels of miRNAs associated with aneuploidy and euploidy, as well as mRNAs related to implantation, can aid in predicting blastocyst chromosomal normality and improving assisted reproductive technology (ART) outcomes.This study analyzed chromosomal abnromality of 25 blastocysts using uorescence in situ hybridization (FISH) and also the expression of genes ERBB4, SELL, ITGB3, and ITGAV, as well as miRNAs, miR-339, miR-27b, miR-661, miR-30c, miR-191, miR-345, miR-142, miR-141, miR-20a and, miR-372. We found that 17 out of 25 embryos were aneuploid. Moreover, results revealed lower expression levels of miR-30c and

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Association of trophectoderm mRNAs and microRNAs with chromosomal aneuploidy of embryo

Esmaeilivand,

Ghasemzadeh,

Niknafs

et al. 2023

Preprint

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MicroRNAs (miRNAs) and mRNAs can serve as indicators of the chromosomal state of an embryo, with different profiles observed in euploid and aneuploid blastocysts. Examining the levels of miRNAs associated with aneuploidy and euploidy, as well as mRNAs related to implantation, can aid in predicting blastocyst chromosomal normality and improving assisted reproductive technology (ART) outcomes. This study analyzed chromosomal abnromality of 25 blastocysts using fluorescence in situ hybridization (FISH) and also the expression of genes ERBB4, SELL, ITGB3, and ITGAV, as well as miRNAs, miR-339, miR-27b, miR-661, miR-30c, miR-191, miR-345, miR-142, miR-141, miR-20a and, miR-372. We found that 17 out of 25 embryos were aneuploid. Moreover, results revealed lower expression levels of miR-30c and miR-372 in aneuploid embryos compared to euploid ones, while ITGAV and ITGB3 showed significantly higher expression in aneuploid embryos. These findings suggest that miR-372, miR-30c, ITGAV, and ITGB3 expression in trophectoderm cells can serve as biomarkers for assessing embryo health.

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microRNAs in the blastocoel fluid as accessible indicators of chromosomal normality

Cited by 4 publications

References 25 publications

Differentially expressed microRNAs in aneuploid preimplantation blastocysts: a systematic review

Differentially expressed microRNAs in aneuploid preimplantation blastocysts: a systematic review

Association of Trophectoderm mRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Association of trophectoderm mRNAs and microRNAs with chromosomal aneuploidy of embryo

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