MicroRNAs mediated regulation of glutathione peroxidase 7 expression and its changes during adipogenesis

Hanousková, Barbora; Vávrová, Gabriela; Ambrož, Martin; Boušová, Iva; Karlsen, Tommy A.; Skálová, Lenka; Matoušková, Petra

doi:10.1016/j.bbagrm.2021.194734

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…As such, H3.3K27M mutation accompanied by increased total H3K27ac and reduction in H3K27me3, leading to glioma formation and subsequent tumor progression in a brainstem glioma model 139 . Lastly, another potential insight into the epigenetic mechanisms related to GPX7 expression comes from our co-expression analyses showing that specific miRNA signatures can regulate GPX7 in gliomas, concurrent with a previous report wherein miR-137 and miR-29b were shown to bind to the 3′ UTR region of GPX7 and inhibit its expression in both SW480 (human colon adenocarcinoma) and HEK293 (human embryonic kidney cell line) cell lines 140 . Thus, the functional relationship between GPX7 expression and epigenetic modifications is complex and further mechanistic studies are required to explain this dependency.…”

Section: Discussionsupporting

confidence: 76%

Comprehensive analysis of epigenetics regulation, prognostic and the correlation with immune infiltrates of GPX7 in adult gliomas

Ferreira

Vitiello

Medina

et al. 2022

Sci Rep

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Gliomas are the most commonly occurring malignant brain tumor characterized by an immunosuppressive microenvironment accompanied by profound epigenetic changes, thus influencing the prognosis. Glutathione peroxidase 7 (GPX7) is essential for regulating reactive oxygen species homeostasis under oxidative stress. However, little is known about the function of GPX7 in gliomas. In this study, we hypothesized that GPX7 methylation status could influence biological functions and local immune responses that ultimately impact prognosis in adult gliomas. We conducted an integrated bioinformatics analysis mining GPX7 DNA methylation status, transcriptional and survival data of glioma patients. We discovered that GPX7 was remarkably increased in glioma tissues and cell lines, and was associated with poor prognosis. This upregulation was significantly linked to clinicopathological and molecular features, besides being expressed in a cell cycle-dependent manner. Our results consistently demonstrated that upregulation of GPX7 is tightly modulated by epigenetic processes, which also impacted the overall survival of patients with low-grade gliomas (LGG). Based on the analysis of biological functions, we found that GPX7 might be involved in immune mechanisms involving both innate and adaptive immunity, type I interferon production and regulation of synaptic transmission in LGG, whereas in GBM, it is mainly related to metabolic regulation of mitochondrial dynamics. We also found that GPX7 strongly correlates with immune cell infiltration and diverse immune cell markers, suggesting its role in tumor-specific immune response and in regulating the migration of immune cell types to the tumor microenvironment. Combining these multiple data, we provided the first evidence regarding the epigenetic-mediated regulatory mechanisms underlying GPX7 activation in gliomas. Furthermore, our study brings key insights into the significant effect of GPX7 in modulating both immune molecules and in immune cell infiltration in the microenvironment of gliomas, which might impact the patient outcome, opening up future opportunities to regulate the local immune response.

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Section: Discussionsupporting

confidence: 76%

Comprehensive analysis of epigenetics regulation, prognostic and the correlation with immune infiltrates of GPX7 in adult gliomas

Ferreira

Vitiello

Medina

et al. 2022

Sci Rep

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“…A recent meta-analysis of 25 individual studies comparing miR expression in CAD patients to healthy controls, counted a total of 239 reported dysregulated miRs, however, after accounting for reproducibility only 48 of them were shown to be significantly dysregulated [ 59 ]. Several of these miRs have been previously associated with one or more traditional CAD risk factors, for example, arterial hypertension [ 60 ], hyperlipidemia [ 61 , 62 , 63 , 64 ], obesity [ 65 , 66 ], COPD [ 60 , 67 ] and diabetes [ 68 , 69 ]. These and other factors such as smoking and physical inactivity have been identified as typical risk factors by Framingham heart study and its follow-ups [ 70 , 71 , 72 , 73 , 74 , 75 ].…”

Section: Discussionmentioning

confidence: 99%

Vascular Tissue Specific miRNA Profiles Reveal Novel Correlations with Risk Factors in Coronary Artery Disease

et al. 2021

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Cardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide. Non-coding RNAs have already been linked to CVD development and progression. While microRNAs (miRs) have been well studied in blood samples, there is little data on tissue-specific miRs in cardiovascular relevant tissues and their relation to cardiovascular risk factors. Tissue-specific miRs derived from Arteria mammaria interna (IMA) from 192 coronary artery disease (CAD) patients undergoing coronary artery bypass grafting (CABG) were analyzed. The aims of the study were 1) to establish a reference atlas which can be utilized for identification of novel diagnostic biomarkers and potential therapeutic targets, and 2) to relate these miRs to cardiovascular risk factors. Overall, 393 individual miRs showed sufficient expression levels and passed quality control for further analysis. We identified 17 miRs–miR-10b-3p, miR-10-5p, miR-17-3p, miR-21-5p, miR-151a-5p, miR-181a-5p, miR-185-5p, miR-194-5p, miR-199a-3p, miR-199b-3p, miR-212-3p, miR-363-3p, miR-548d-5p, miR-744-5p, miR-3117-3p, miR-5683 and miR-5701–significantly correlated with cardiovascular risk factors (correlation coefficient >0.2 in both directions, p-value (p < 0.006, false discovery rate (FDR) <0.05). Of particular interest, miR-5701 was positively correlated with hypertension, hypercholesterolemia, and diabetes. In addition, we found that miR-629-5p and miR-98-5p were significantly correlated with acute myocardial infarction. We provide a first atlas of miR profiles in IMA samples from CAD patients. In perspective, these miRs might play an important role in improved risk assessment, mechanistic disease understanding and local therapy of CAD.

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“…GPX5 is secreted in the epididymis and spermatozoa, while GPX6 is localized in the olfactory epithelium [ 18 , 19 ]. GPX7 and GPX8, both localized in the endoplasmic reticulum, share many characteristics: both contain cysteine instead of selenocysteine in their catalytic centers and exhibit minimal GPX activity due to the absence of a GSH-binding domain [ 20 , 21 ]. Many cysteine-based GPX-homologous sequences have been discovered, which do not rely on GSH as a reductant but prefer redoxins characterized by a CxxC motif [ 22 ].…”

Section: Gpxsmentioning

confidence: 99%

Insights into the Role of Glutathione Peroxidase 3 in Non-Neoplastic Diseases

Zhang,

Liao,

Lin

et al. 2024

Biomolecules

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Reactive oxygen species (ROSs) are byproducts of normal cellular metabolism and play pivotal roles in various physiological processes. Disruptions in the balance between ROS levels and the body’s antioxidant defenses can lead to the development of numerous diseases. Glutathione peroxidase 3 (GPX3), a key component of the body’s antioxidant system, is an oxidoreductase enzyme. GPX3 mitigates oxidative damage by catalyzing the conversion of hydrogen peroxide into water. Beyond its antioxidant function, GPX3 is vital in regulating metabolism, modulating cell growth, inducing apoptosis and facilitating signal transduction. It also serves as a significant tumor suppressor in various cancers. Recent studies have revealed aberrant expression of GPX3 in several non-neoplastic diseases, associating it with multiple pathological processes. This review synthesizes the current understanding of GPX3 expression and regulation, highlighting its extensive roles in noncancerous diseases. Additionally, this paper evaluates the potential of GPX3 as a diagnostic biomarker and explores emerging therapeutic strategies targeting this enzyme, offering potential avenues for future clinical treatment of non-neoplastic conditions.

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MicroRNAs mediated regulation of glutathione peroxidase 7 expression and its changes during adipogenesis

Cited by 7 publications

References 40 publications

Comprehensive analysis of epigenetics regulation, prognostic and the correlation with immune infiltrates of GPX7 in adult gliomas

Comprehensive analysis of epigenetics regulation, prognostic and the correlation with immune infiltrates of GPX7 in adult gliomas

Vascular Tissue Specific miRNA Profiles Reveal Novel Correlations with Risk Factors in Coronary Artery Disease

Insights into the Role of Glutathione Peroxidase 3 in Non-Neoplastic Diseases

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