MicroRNAs transfected into granulosa cells may regulate oocyte meiotic competence during in vitro maturation of mouse follicles

Abstract

This work was supported by a grant from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A111539). None of the authors has any conflicts of interest to declare.

Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

“…Furthermore, in silico prediction showed that miR-451 may target genes such as TSC1, whose absence in the murine model causes POF (50); SPC25, which induces spindle misalignment in knockout murine models (51); and EREG (also known as epiregulin), which has also been tested as an inductor peptide for cumulus-oocytes in in vitro maturation protocols (52). In the murine model, it has also been found that miR-27a expression is increased in GCs from MII versus MI murine oocytes (53). These results are in accordance with our observations for hsa-miR-27b, a miR-27a homolog, which is expressed in higher amounts in GV FF than in MII FF.…”

Effects of Estradiol on the Paracrine Regulator Expression of In Vitro Maturated Murine Ovarian Follicles

“…The methods used for OFIVM have been modified from previous studies [16,17]. For the experiment of OFIVM, early stage pre-antral follicles were mechanically separated using a syringe needle.…”

Hypoxia induces miR-210, leading to anti-apoptosis in ovarian follicular cells of marine medaka Oryzias melastigma

“…NAs play an important role in ovarian development and function through regulating post-transcriptional gene expression (Baley and Li, 2012;Kim et al, 2013;Lei et al, 2010). miR-210 is one of the most studied hypoxia-inducible miRNAs (Cicchillitti et al, 2012), and accumulating evidence suggests an important role for miR-210 in cell apoptosis.…”