Microsatellite analysis of environmental and clinical isolates of the opportunist fungal pathogen Aspergillus fumigatus

Rosehart, Kimberly; Richards, Miriam H.; Bidochka, Michael

doi:10.1099/0022-1317-51-12-1128

Cited by 25 publications

(17 citation statements)

References 16 publications

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“…Our result is consistent with earlier studies of more limited scope, for example, no correlation was found between genotype and geography for European and North American individuals (Debeaupuis et al 1997), nor for isolates from French and Italian patients (Bertout et al 2001), nor for environmental and clinical isolates from Canada (Rosehart et al 2002). Neither has any correlation between genotype and geography been found in another, study using a different collection of A. fumigatus individuals and different genetic markers (Rydholm et al 2003).…”

Section: Biogeographysupporting

confidence: 83%

“…Our data support the hypothesis that any isolate of either the ''fumigatus'' or the ''occultum'' clade is a potential pathogen, an idea originally formulated from European and North American collections of A. fumigatus (Debeaupuis et al 1997, Bart-Delabesse et al 1998, see also Rosehart et al 2002). These taxa are accidental human pathogens and are not transmitted from host to host; infection provides no apparent benefit to the fungi.…”

Section: Cryptic Speciessupporting

confidence: 74%

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Cryptic Speciation in the Cosmopolitan and Clonal Human Pathogenic Fungus Aspergillus Fumigatus

et al. 2005

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Abstract. Microbes and other organisms smaller than one to a few millimeters in size are hypothesized to have global populations, in contrast to the geographically restricted ranges of larger organisms. However, fungi, which routinely have reproductive propagules no larger than 10 micrometers, challenge the generality of this hypothesis because recent studies have shown that globally distributed morphological species embrace two or more geographically restricted phylogenetic species. We used the concordance of gene genealogies to recognize phylogenetic species in the globally distributed opportunistic human pathogenic fungus, Aspergillus fumigatus. Based on DNA sequence data of five loci for each of 63 individuals collected from five continents, we have delineated two phylogenetic species in this single morphological species. Unlike all other fungi examined to date, both genetically isolated groups showed a global distribution with no evidence of a correlation between genotype and geographic location. Sexual reproduction has never been observed in A. fumigatus, but when the same data were used to explore the association of alleles at the five loci for one of the phylogenetic species, evidence was found to support recombination. The discovery of a cryptic species is medically relevant because different species are likely to differ in virulence or drug resistance. The discovery of a globally distributed A. fumigatus species clade highlights the need for ecological studies of the fungus to either document global dispersal or propose alternative mechanisms by which it persists as single, global phylogenetic population.

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Section: Biogeographysupporting

confidence: 83%

Section: Cryptic Speciessupporting

confidence: 74%

Cryptic Speciation in the Cosmopolitan and Clonal Human Pathogenic Fungus Aspergillus Fumigatus

et al. 2005

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“…By far, the most successful typing approach for A. fumigatus has been microsatellite, or short tandem repeat, analysis. This method has been used successfully by several investigators (4,14,19,43,51) and has been shown to have a discriminatory power of 0.99 and better (4,14).…”

Section: Discussionmentioning

confidence: 99%

Multilocus Sequence Typing of the Pathogenic Fungus Aspergillus fumigatus

et al. 2007

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A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.

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“…In comparison with mammals, birds are highly susceptible to respiratory infection with Aspergillus fumigatus (Tell, 2005), and pulmonary invasive aspergillosis is a common disease in birds, causing significant economic losses in the poultry industry, with up to 30 % fatality (Zafra et al, 2008). While outbreaks of human invasive aspergillosis in hospital settings have been studied by several groups (Bertout et al, 2001;Chazalet et al, 1998;Rosehart et al, 2002), only one study focused on the epidemiology of aspergillosis in poultry (Lair-Fulleringer et al, 2003), and another recently in captive penguins (Alvarez-Perez et al, 2010). In these reports, Aspergillus flavus was second only to A. fumigatus, which accounted for approximately 95 % of avian infections.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite typing of Aspergillus flavus from clinical and environmental avian isolates

Hadrich

Drira

Neji

et al. 2013

Journal of Medical Microbiology

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Aspergillosis is one of the most common causes of death in captive birds. Aspergillus fumigatus accounts for approximately 95 % of aspergillosis cases and Aspergillus flavus is the second most frequent organism associated with avian infections. In the present study, the fungi were grown from avian clinical samples (post-mortem lung material) and environmental samples (eggs, food and litter). Microsatellite markers were used to type seven clinical avian isolates and 22 environmental isolates of A. flavus. A. flavus was the only species (28 % prevalence) detected in the avian clinical isolates, whereas this species ranked third (19 %) after members of the genera Penicillium (39 %) and Cladosporium (21 %) in the environmental samples. Upon microsatellite analysis, five to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a 0.852 D value. The combination of all six markers yielded a 0.991 D value with 25 distinct genotypes. One clinical avian isolate (lung biopsy) and one environmental isolate (egg) shared the same genotype. Microsatellite typing of A. flavus grown from avian and environmental samples displayed an excellent discriminatory power and 100 % reproducibility. This study showed a clustering of clinical and environmental isolates, which were clearly separated. Based upon these results, aspergillosis in birds may be induced by a great diversity of isolates.

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Microsatellite analysis of environmental and clinical isolates of the opportunist fungal pathogen Aspergillus fumigatus

Cited by 25 publications

References 16 publications

Cryptic Speciation in the Cosmopolitan and Clonal Human Pathogenic Fungus Aspergillus Fumigatus

Cryptic Speciation in the Cosmopolitan and Clonal Human Pathogenic Fungus Aspergillus Fumigatus

Multilocus Sequence Typing of the Pathogenic Fungus Aspergillus fumigatus

Microsatellite typing of Aspergillus flavus from clinical and environmental avian isolates

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