Microsatellite based assignment reveals history of extirpated mountain ungulate

Safner, Toni; Bužan, Elena; Iacolina, Laura; Potušek, Sandra; Rezić, Andrea; Sindičić, Magda; Kavčić, Krešimir; Šprem, Nikica

doi:10.1007/s10709-020-00084-5

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“…Bone structures that are equipped with attributive data on individuals enable us to study the spatiotemporal variability within and among populations and to perform retrospective studies (e.g. mandibles in different environmental studies: Kierdorf and Kierdorf 2000 , 2005 ; Jelenko and Pokorny 2010 ; antlers/horns in genetic studies: Hoffmann and Griebeler 2013 ; Giżejewska et al 2016 ; Safner et al 2020 ). These bones are usually kept for decades or even centuries in well-preserved public (museums) and private collections, which may cover different regions and historical periods, and in which data on the origin of individuals, year of death and age are also often recorded.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterisation of wild ungulates: successful isolation and analysis of DNA from widely available bones can be cheap, fast and easy

Bužan¹,

Potušek²,

Urzi³

et al. 2020

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Genetic characterisation of wild ungulates can be a useful tool in wildlife management and in obtaining a greater understanding of their biological and ecological roles in a wider spatiotemporal context. Different ways of optimising methodologies and reducing the costs of genetic analyses using widely available bone tissues collected within regular hunting allocations were examined. Successful isolation and analysis of DNA from widely available bones can be cheap, fast and easy. In particular, this study explored the possibility of using bones for extracting high quality nuclear DNA for microsatellite analysis. The utility of applying a modified demineralisation process using two commercially available DNA isolation kits, which differ significantly in price, was evaluated. The sample sets included bones and, for comparison, muscle tissues from four wild ungulate species: chamois (Rupicapra rupicapra), roe deer (Capreolus capreolus), wild boar (Sus scrofa), and Alpine ibex (Capra ibex). For the recent bones, these results confirmed that the DNA concentrations and microsatellite amplification were sufficiently high, even when using low-cost kits, after prior demineralisation. For old bones, prior demineralisation and use of a specially designed isolation kit led to a more successful extraction of DNA. Besides reducing kit-related costs, low-cost kits are much faster and therefore make genetic analysis more efficient.

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