Microsatellite instability in follicle centre cell lymphoma

Abstract

Fluorescent polymerase chain reaction (PCR) was used to assay 12 microsatellite markers (APC x 2, DCC, P53 x 2, RB1, NM23, WT1, D6S260, D6S262, D6S281 and TNFa) to look for evidence of microsatellite instability in 40 cases of follicle centre cell lymphoma (FCC). Evidence of novel alleles seen in the tumour tissue but not the normal uninvolved tissue was seen in seven cases (17%). In only two of these cases (5%) was more than one locus involved but in these cases multiple affected loci were seen (4/12 and 7/12 respectively). The detection of microsatellite instability indicates a DNA repair defect such as that which would be predicted to occur in cells with mutated mismatch repair genes, a novel finding in FCC lymphoma.

BAX frameshift mutations in cell lines derived from human haemopoietic malignancies are associated with resistance to apoptosis and microsatellite instability

“…Although MI is not thought to be a common feature of haemopoietic malignancies (Gartehnhaus et al, 1996;Larson et al, 1996;Randerson et al, 1996;Robledo et al, 1995;Volpe et al, 1996), there are clear examples of leukaemia and lymphoma with MI. For example, approximately 50% of human immunode®ciency virus-associated non-Hodgkin's lymphoma and 90% of therapy related leukaemia exhibit instability of at least 25% of markers tested (Bedi et al, 1995;BenYehuda et al, 1996).…”

Analysis of the frequency of microsatellite instability and p53 gene mutation in splenic marginal zone and MALT lymphomas

“…[22][23][24] DiVerent studies of the frequency of microsatellite instability in lymphoproliferative disorders have shown this to be an exceptional finding, with the exclusive exception of MALT lymphomas, where microsatellite instability has been identified in up to 52.5% of patients. [25][26][27][28][29][30][31] On the other hand, p53 mutation is one of the most common genetic alterations in human malignancies, contributing to tumour development and progression. 32 The frequency of p53 mutation in lymphoid disorders varies from 5% to 42%.…”

True

“…These data are consistent with studies indicating that microsatellite instability and mismatch repair deficiency are rare in various non-Hodgkin's lymphomas. 32,33 Recently, however, various studies suggested an association between MSI and certain subsets of lymphoid malignancies. These specifically include chronic infection and/or inflammation-related lymphomas such as Epstein-Barr virus (EBV)-associated lymphomas in AIDS patients and posttransplant patients as well as the EBV and Herpes Simplex Virus/HH8-associated body cavity lymphomas in AIDS patients.…”