Microsatellite marker panels for use in high-throughput genotyping of mouse crosses

Iakoubova, Olga A.; Olsson, Christine; Dains, Katherine M.; Choi, Jim; Kalcheva, Ivetta; Bentley, L. Gordon; Cunanan, Madalyne; Hillman, David R.; Louie, Judi; Machrus, M.; West, David B.

doi:10.1152/physiolgenomics.2000.3.3.145

Cited by 13 publications

(10 citation statements)

References 18 publications

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“…Tail tips from parentals and F 1 and F 2 mice were digested with proteinase K and DNA precipitated with ethanol. Fluorescently labeled primers corresponding to 350 markers shown to be polymorphic between C57BL͞6Jand CAST͞Ei by Iakoubova et al (10) were tested to see whether they were also polymorphic between C57BL͞6J and CASA͞Rk, resulting in the identification of 255 markers that were used for genotyping in the current cross. The average spacing between these markers was 5.9 cM.…”

Section: Methodsmentioning

confidence: 99%

Loci on chromosomes 14 and 2, distinct from ABCG5/ABCG8, regulate plasma plant sterol levels in a C57BL/6J × CASA/Rk intercross

Sehayek

Duncan

Lütjohann

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Plasma plant sterol levels differ among humans due to genetic and dietary factors. A disease characterized by high plasma plant sterol levels, ␤-sitosterolemia, was recently found to be due to mutations at the ABCG5͞ABCG8 locus. To detect variants at this and other loci, a genetic cross was carried out between two laboratory mouse strains. Parental C57BL͞6J had almost twice the campesterol and sitosterol levels compared with parental CASA͞Rk mice, and F1 mice had levels halfway between the parentals. An intercross between F1s was performed and plasma plant sterol levels measured in 102 male and 99 female F2 mice. Plasma plant sterols in F2s displayed a unimodal distribution, suggesting the effects of several rather a single major gene. In the F2 mice, a full genome scan revealed significant linkages on chromosomes 14 and 2. With regard to chromosome 14, analysis showed a single peak for linkage at 17 cM with a logarithm of odds (LOD) score of 9.9, designated plasma plant sterol 14 (Plast14). With regard to chromosome 2, analysis showed two significant peaks for linkage at 18 and 65 cMs with LOD scores of 4.1 and 3.65, respectively, designated Plast2a and Plast2b, respectively. Four interactions between loci, predominantly of an additive nature, were also demonstrated, the most significant between Plast14 and Plast2b (LOD 16.44). No significant linkage or gene interaction was detected for the ABCG5͞ABCG8 locus on chromosome 17. Therefore, other genes besides ABCG5͞ABCG8 influence plasma plant sterol levels and now become candidates to explain differences in plasma plant sterol levels between humans.

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Section: Methodsmentioning

confidence: 99%

Loci on chromosomes 14 and 2, distinct from ABCG5/ABCG8, regulate plasma plant sterol levels in a C57BL/6J × CASA/Rk intercross

Sehayek

Duncan

Lütjohann

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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“…To determine whether these effects represented a global influence on X chromosome choice (regardless of Xce genotype), we used a QTL mapping strategy in a population of B6CASTF 2 Xce homozygotes and Xce heterozygotes. We genotyped 72 B6CASTF 2 Xce heterozygous and 28 B6CASTF 2 Xce homozygous females using a panel of microsatellite markers with 20 cM average spacing based on one developed previously (Iakoubova et al 2000). We identified a single major QTL (Figure 1) in this cross.…”

Section: Identification Of Major Genetic Influences On X Inactivationmentioning

confidence: 99%

Genetic Control of X Chromosome Inactivation in Mice: Definition of the Xce Candidate Interval

et al. 2006

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In early mammalian development, one of the two X chromosomes is silenced in each female cell as a result of X chromosome inactivation, the mammalian dosage compensation mechanism. In the mouse epiblast, the choice of which chromosome is inactivated is essentially random, but can be biased by alleles at the X-linked X controlling element (Xce). Although this locus was first described nearly four decades ago, the identity and precise genomic localization of Xce remains elusive. Within the X inactivation center region of the X chromosome, previous linkage disequilibrium studies comparing strains of known Xce genotypes have suggested that Xce is physically distinct from Xist, although this has not yet been established by genetic mapping or progeny testing. In this report, we used quantitative trait locus (QTL) mapping strategies to define the minimal Xce candidate interval. Subsequent analysis of recombinant chromosomes allowed for the establishment of a maximum 1.85-Mb candidate region for the Xce locus. Finally, we use QTL approaches in an effort to identify additional modifiers of the X chromosome choice, as we have previously demonstrated that choice in Xce heterozygous females is significantly influenced by genetic variation present on autosomes (Chadwick and Willard 2005). We did not identify any autosomal loci with significant associations and thus show conclusively that Xce is the only major locus to influence X inactivation patterns in the crosses analyzed. This study provides a foundation for future analyses into the genetic control of X chromosome inactivation and defines a 1.85-Mb interval encompassing all the major elements of the Xce locus.

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“…Most molecular markers designed for such studies have been described for classical laboratory strains (Dietrich et al . 1996; Iakoubova et al . 2000) carrying a mixture of various mouse genomes (Wade et al .…”

Section: Panel Of Microsatellite Multiplexes (A) and The List Of Primmentioning

confidence: 99%

Polymerase chain reaction multiplexing of microsatellites and single nucleotide polymorphism markers for quantitative trait loci mapping of wild house mice

Kawałko

Dufková

Wójcik

et al. 2009

Molecular Ecology Resources

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We tested 96 microsatellites and 10 single nucleotide polymorphisms for their allelic distribution in two subspecies of the house mouse, Mus musculus musculus and M. m. domesticus. Sixty-two microsatellites discriminated strain-specific differences among nine wild-derived 'musculus' and 'domesticus' and three 'classical' laboratory strains. For efficient genotyping, we optimized multiplex conditions using five microsatellites per polymerase chain reaction. All 10 single nucleotide polymorphisms were also optimized for simultaneous analysis in one reaction using SNaPshot multiplex. The uniform distribution of markers on autosomes and on the X chromosome makes these panels potentially useful tools for quantitative trait loci mapping of wild house mice.

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Microsatellite marker panels for use in high-throughput genotyping of mouse crosses

Cited by 13 publications

References 18 publications

Loci on chromosomes 14 and 2, distinct from ABCG5/ABCG8, regulate plasma plant sterol levels in a C57BL/6J × CASA/Rk intercross

Loci on chromosomes 14 and 2, distinct from ABCG5/ABCG8, regulate plasma plant sterol levels in a C57BL/6J × CASA/Rk intercross

Genetic Control of X Chromosome Inactivation in Mice: Definition of the Xce Candidate Interval

Polymerase chain reaction multiplexing of microsatellites and single nucleotide polymorphism markers for quantitative trait loci mapping of wild house mice

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