Microsatellite markers for evolutionary studies in the sexually deceptive orchid genus Chiloglottis

Abstract

The orchids in the genus Chiloglottis are pollinated exclusively by sexual deception. We describe 20 variable microsatellite loci that will facilitate the study of the evolution and ecology of this specialized pollination system. Two loci were isolated from Chiloglottis trilabra, with the rest derived from Chiloglottis valida. All loci were polymorphic in C. valida (HE 0.07–0.87). Most loci amplified, and were variable within close relatives of C. valida, and a number amplified more broadly, including outside the genus.

Pollination by sexual deception promotes outcrossing and mate diversity in self‐compatible clonal orchids

“…We therefore carried out a genotyping screen of a mixed‐taxon pool of 200 leaf samples from the transect at 12 microsatellite loci described in Flanagan et al . (). We then ranked loci in order of information content as measured by the effective number of alleles and chose the six most informative loci for genotyping in further analyses.…”

“…As the amount of tissue in early-stage protocorms is very small, the resulting DNA yields were also low and necessitated a trade-off between the number of loci genotyped against the amount of DNA required for reliable amplification and repeats if necessary. We therefore carried out a genotyping screen of a mixed-taxon pool of 200 leaf samples from the transect at 12 microsatellite loci described in Flanagan et al (2006). We then ranked loci in order of information content as measured by the effective number of alleles and chose the six most informative loci for genotyping in further analyses.…”

Microsatellite loci for a new Australian endemic plant species Erythroxylum sp. ‘Cholmondely Creek’ (J.R.Clarkson 9367) (Erythroxylaceae)

“…We thus Genomic DNA from three samples extracted from silica dried leaf material using the QIAGEN Tissue Lyser and QIAGEN DNeasy Plant mini kit, were pooled then purified and concentrated using SureClean (Bioline). The method of Glenn and Schable (2005) (modified by Flanagan et al 2006) was used to construct a microsatellite AG-enriched library. Polymerase chain reactions (PCRs) were carried out on 192 clones and from these 189 sequences were obtained using M13 primers.…”

Isolation and characterization of microsatellite markers for the waratah, Telopea speciosissima (Proteaceae)

“…Leaf tissue from one T. speciosissima plant was extracted using a QIAGEN DNeasy plant mini kit to obtain total genomic DNA. The microsatellite‐enriched library was constructed using the protocol described by Glenn & Schable (2005) with modifications outlined in Flanagan et al . (2006).…”