Microsatellite markers for the Mexican spotted owl (<i>Strix occidentalis lucida</i>)

Thode, Anna B.; Maltbie, Mary; Hansen, Leslie A.; Green, Lance D.; Longmire, Jonathan L.

doi:10.1046/j.1471-8286.2002.00267.x

Cited by 23 publications

(27 citation statements)

References 6 publications

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“…Some of this variation among families is certainly a function of time and resources expended as hundreds of microsatellites have been identified in the agronomically important chicken (personal communication, H. Cheng, USDA East Lansing, MI, USA). However, other studies, comparable to ours have reaped substantially greater numbers of markers (Isaksson & Tegelstrom 2002;Korfanta et al 2002;Thode et al 2002). Only two studies have identified polymorphic microsatellite loci in Laridae (Given et al 2002;Tirard et al 2002).…”

Section: Discussionsupporting

confidence: 70%

Novel microsatellite markers used to determine the population genetic structure of the endangered Roseate Tern, Sterna dougallii, in Northwest Atlanticand Western Australia

2005

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The Roseate Tern, Sterna dougallii, is an endangered species in the Northwest Atlantic, where it has undergone transient reductions in population size over the past 120 years. This population has been slow to regain former size and range, perhaps in part due to the female-biased sex ratio, which results in female-female pairs, reducing the average productivity of the colony. The larger populations of the Western Pacific and Indian Oceans are not endangered and there is no evidence of a biased sex ratio at breeding in Western Australia. We developed four novel microsatellite markers and adapted one other and these are the first used in the genus Sterna. We also determined the utility of these markers for 17 related species. Here we report the population genetic structure within and between two regions, the Northwest Atlantic and Western Australia. A significant finding is that the Northwestern Atlantic region has much lower allelic diversity than the Western Australia region, promoting the recommendation for increased protection of sites in this region in order to preserve remaining genetic diversity and new potential breeding habitats.

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Section: Discussionsupporting

confidence: 70%

Novel microsatellite markers used to determine the population genetic structure of the endangered Roseate Tern, Sterna dougallii, in Northwest Atlanticand Western Australia

2005

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“…All owls were genotyped at 10 variable microsatellite loci (Table 2) developed for Mexican spotted owls (S. o. lucida; loci: 6H8, 15A6, 13D8, 4E10.2; Thode et al 2002), Lanyu scops owls (Otus elegans botelensis; loci: Oe3-7, Oe053, Oe128, Oe129, Oe149; Hsu et al 2003Hsu et al , 2006, and ferruginous pygmy-owls (Glaucidium brasilianum; locus: FEPO5; Proudfoot et al 2005). One of these loci (Oe128) and an additional microsatellite marker (Bb126) are diagnostic of spotted versus barred owls (Funk et al 2007) and were genotyped to assure that no barred owls or spotted owlbarred owl hybrids were included.…”

Section: Samplingmentioning

confidence: 99%

Evidence for recent population bottlenecks in northern spotted owls (Strix occidentalis caurina)

et al. 2009

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The northern spotted owl (Strix occidentalis caurina) is one of the most controversial threatened subspecies ever listed under the US Endangered Species Act. Despite protection of its remaining forest habitat, recent field studies show continued declines of northern spotted owls. One potential threat to northern spotted owls which has not yet been shown is loss of genetic variation from population bottlenecks. Bottlenecks can increase the probability of mating among related individuals, potentially causing inbreeding depression, and can decrease adaptive potential. Here we report evidence for recent bottlenecks in northern spotted owls using a large genetic dataset (352 individuals and 10 microsatellite loci). The signature of bottlenecks was strongest in the Washington Cascade Mountains, in agreement with field data. Our results provide independent evidence that northern spotted owls have recently declined, and suggest that loss of genetic variation is an emerging threat to the subspecies' persistence. Reduced effective population size (N e ) shown here in addition to field evidence for demographic decline highlights the increasing vulnerability of this bird to extinction.

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“…Ten polymorphic microsatellite loci designed for pygmy-owls (Proudfoot et al 2005) and one locus (4E10.2) from the Mexican spotted owl (Thode et al 2002) were used to derive a genotype for each individual and to assess genetic variation within and between all populations. The 5¢-end of each forward primer was labeled with a fluorescent marker for genotyping on an ABI PRISM 377 automated DNA sequencer (Applied Biosystems, Foster City, California).…”

Section: Data Collectionmentioning

confidence: 99%

“…In this study, we examine patterns of genetic variation at 11 polymorphic microsatellite loci, 10 of which were isolated from the pygmy-owl (Proudfoot et al 2005) and one from the Mexican spotted owl (Strix occidentalis lucida, Thode et al 2002). Microsatellite markers exhibit high levels of genetic variation and have proven useful for detailed genetic studies within avian species (Barrowclough and Gutie´rrez 1990;Larson et al 2002;Pe´rez et al 2002;Csiki et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Variation in DNA microsatellites of the ferruginous pygmy-owl (Glaucidium brasilianum)

et al. 2006

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Eleven polymorphic microsatellite loci were used to assess genetic variation in the ferruginous pygmy owl (Glaucidium brasilianum) from North America. Analysis of genotypic variation suggests restricted gene flow between pygmy-owl populations in Arizona-Sonora and Sinaloa, and Texas and the remaining states in Mexico. The Arizona-Sonora population showed signs of a recent genetic bottleneck, an observation supported by low population estimates for Arizona (13-117 individuals). Heterozygosity in Arizona, however, was equal to levels recorded throughout Mexico and Texas. Congruent patterns revealed by nuclear (microsatellites) and mitochondrial DNA that indicate Arizona-Sonora and Texas populations are distinct from adjacent populations in Mexico, thus emphasizing need for the design and implementation of separate management plans for recovery and conservation efforts. Revealing evidence of distinct groups within the pygmy-owl populations in North America, results from this study may be used to make management decisions for the recovery and conservation of this species.

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Microsatellite markers for the Mexican spotted owl (Strix occidentalis lucida)

Cited by 23 publications

References 6 publications

Novel microsatellite markers used to determine the population genetic structure of the endangered Roseate Tern, Sterna dougallii, in Northwest Atlanticand Western Australia

Novel microsatellite markers used to determine the population genetic structure of the endangered Roseate Tern, Sterna dougallii, in Northwest Atlanticand Western Australia

Evidence for recent population bottlenecks in northern spotted owls (Strix occidentalis caurina)

Variation in DNA microsatellites of the ferruginous pygmy-owl (Glaucidium brasilianum)

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