Microsatellite Markers in the Population from Rio Grande Do Norte, Northeastern Brazil

Abstract

Samples of venous blood were collected using the anticoagulant ACD (citric acid 0.038 M; tribasic sodium citrate 0.075 M; dextrose 0.033 M), from 247 non-related, healthy individuals from the population of the State of Rio Grande do Norte, in Northeastern Brazil. The DNA was extracted by rapid mini salting-out and digestion with proteinase K (1). The PCR was performed in a Perkin-Elmer 2400 thermal cycler, using amplification protocols described in Promega Manual (Madison, WI). The amplified products underwent electrophoresis in a denaturing polyacrylamide gel in a manual sequencer from Amershan Pharmacia (SQ3 Sequencer). The statistical analysis was performed using the Genetic Data Analysis (2) and the PowerStat (3) softwares. The complete dataset is available in www.nlink.com.br/mauricio.

13 STR loci frequencies in the population from Paraíba, Northeast Brazil

“…The population in the Northeast Region can be characterized as mixed, having originated from African, Caucasian and Native American ancestral individuals. This study is related to a long-term project of investigation of people from this region [1][2][3][4][5][6][7].…”

“…Comparisons involved five other populations [1][2][3][4][5][6][7] of Northeast Brazil, one of Portugal and one of Andalusia [14] (Fig. 2).…”

Genetic analysis of 13 STR loci in the population from the State of Pernambuco, northeast Brazil

“…Population: The Brazilian populations [1][2][3][4][5] have been studied for the loci CSF1PO, TPOX, TH01, F13A1, vWA, D16S539, D7S820, D13S317, D18S51, D21S11, and D8S1179 and this has been the first paper on the northeast Brazilian population encompassing this number of loci. As well as the State of Piaui [1] and of the Rio Grande do Norte [2], the population of the State of Pernambuco can be characterized as an exogamic population, composed of individuals of African, Caucasian and Native American ancestry individuals, and for this reason we have choose this population for our studies.…”

“…As well as the State of Piaui [1] and of the Rio Grande do Norte [2], the population of the State of Pernambuco can be characterized as an exogamic population, composed of individuals of African, Caucasian and Native American ancestry individuals, and for this reason we have choose this population for our studies. Blood sample from 546 unrelated individuals were provided from the paternity caseworks (mothers and fathers) of all State of Pernambuco.…”

“…The comparison of the distribution of the allelic frequency of the CSF1PO, TPOX, TH01, D7S820 and D13S317 loci of the population from Pernambuco with others Brazilian populations [1,2,10,11] show that our population is genetically more proximal of the population of the State of Alagoas [10] (d ¼ 0:0002) and genetically more distant of the population of African-derived Brazilian from the State of São Paulo [11] (d ¼ 0:0225). The forensic parameters regarding the 13 STR loci are useful in terms of variability, paternity and forensic purposes for the Pernambuco population.…”