Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes

Spesso, María Florencia; Nuncira, Carmen T.; Burstein, Verónica Liliana; Masih, Diana T.; Dib, M. D.; Chiapello, Laura S.

doi:10.1007/s10096-013-1839-3

Cited by 21 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…39,52 This epidemiological pattern of clustered cases and high incidence in a short period of time is better known for viral 53 and bacterial pathogens; 54 such events caused by fungi are very rare in humans and are mainly limited to dermatophytes. 55 The emergence of fungal pathogens in other animals is mostly related to a recent introduction or shift in host, as in the case of white-nose syndrome in bats caused by Geomyces destructans , 56 lethargic crab disease by Exophiala cancerae 57 and the devastation of amphibian populations caused by the chytridiomycete Batrachochytrium dendrobatidis . 58,59 …”

Section: Discussionmentioning

confidence: 99%

Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species

Rodrigues

Hoog

Zhang

et al. 2014

Emerging Microbes & Infections

115

195

View full text Add to dashboard Cite

Sporotrichosis, caused by agents of the fungal genus Sporothrix, occurs worldwide, but the infectious species are not evenly distributed. Sporothrix propagules usually gain entry into the warm-blooded host through minor trauma to the skin from contaminated plant debris or through scratches or bites from felines carrying the disease, generally in the form of outbreaks. Over the last decade, sporotrichosis has changed from a relatively obscure endemic infection to an epidemic zoonotic health problem. We evaluated the impact of the feline host on the epidemiology, spatial distribution, prevalence and genetic diversity of human sporotrichosis. Nuclear and mitochondrial markers revealed large structural genetic differences between S. brasiliensis and S. schenckii populations, suggesting that the interplay of host, pathogen and environment has a structuring effect on the diversity, frequency and distribution of Sporothrix species. Phylogenetic data support a recent habitat shift within S. brasiliensis from plant to cat that seems to have occurred in southeastern Brazil and is responsible for its emergence. A clonal structure was found in the early expansionary phase of the cat–human epidemic. However, the prevalent recombination structure in the plant-associated pathogen S. schenckii generates a diversity of genotypes that did not show any significant increase in frequency as etiological agents of human infection over time. These results suggest that closely related pathogens can follow different strategies in epidemics. Thus, species-specific types of transmission may require distinct public health strategies for disease control.

show abstract

Section: Discussionmentioning

confidence: 99%

Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species

Rodrigues

Hoog

Zhang

et al. 2014

Emerging Microbes & Infections

115

195

View full text Add to dashboard Cite

show abstract

“…RAPD and ISSR-PCR methods have frequently been used for phylogenetic analysis and identification of dermatophytes (Kim et al, 1999(Kim et al, , 2001Cano et al, 2005;Leibner-Ciszak et al, 2010). Spesso et al (2013) demonstrated that the detection of intra-species polymorphisms in isolates of M. canis by RAPD-PCR may be applied in future molecular epidemiological studies in order to identify endemic strains, the route of infection in an outbreak and the coexistence of different strains in a single infection. In contrast to that reported and using the same method, Leibner-Ciszak et al (2010) were not able to detect genetic variations in 13 clinical isolates of M. canis.…”

Section: T Mentagrophytes (E)mentioning

confidence: 99%

“…However, outside factors such as temperature variation, medium and chemotherapy, can greatly influence the phenotypic characteristic and consequently can make the identification more difficult (Weitzman and Summerbell, 1995;Faggi et al, 2001;Kanbe, 2008;De Baere et al, 2010). Recently, molecular marker approaches, such as nested-polymerase chain reaction (PCR) (Verrier et al, 2013), random amplified polymorphic DNA (RAPD)-PCR (Kim et al, 2001;Baeza et al, 2006;Leibner-Ciszak et al, 2010;Dobrowolska et al, 2011;Spesso et al, 2013), inter-simple sequence repeat (ISSR)-PCR (Cano et al, 2005;Khosrav et al, 2012), PCR-restriction fragment length polymorphism (RFLP) (Yang et al, 2008;RezaeiMatehkolaei et al, 2012;Samuel et al, 2013), real time PCR (Bergmans et al, 2010) and multiplex PCR assay (Arabatzis et al, 2007;Kim et al, 2011) and others have been adapted for detection of dermatophytes from clinical specimens.…”

Section: Introductionmentioning

confidence: 99%

“…RAPD methods have frequently been used for phylogenetic analysis and identification of dermatophytes. Spesso et al (2013) used random amplified polymorphic DNA primers to recognise Microsporum canis, Microsporum gypseum, Trichophyton rubrum and Trichophyton interdigitale. For identification of Trichophyton mentagrophytes and Trichophyton tonsurans, Kim et al (1999 and proposed a RAPD method, in which T. mentagrophytes was divided into subtypes on the basis of amplification patterns in RAPD using three primers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Abdel-Fatah¹,

Moharram²,

Moubasher³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Three molecular techniques random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and restriction fragment length polymorphism (RFLP) were employed for identification and to study the genetic relationship among six species of dermatophytes and three species of yeasts isolated from Egyptian and Libyan patients with skin mycosis. Each species was represented by two isolates, one from Egyptian patients and the second from Libyan. RAPD in which four random 10-mer primers and two ISSR primers were used to amplify the DNA fragments of target fungi and RFLP in which two universal primers (ITS1 and ITS4) were used to amplify the internal transcribed spacer (ITS) regions of the ribosomal (rRNA) gene in fungal isolates followed by digestion with HinfI and HaeIII endonucleases was carried out. Three molecular marker techniques showed considerable potential for identifying and discriminating dermatophytes and Candida species and the achieved results confirmed identification based on conventional morphological methods. Results of RAPD and ISSR markers revealed 78.7% genetic similarity (GS) between Microsporum canis and other tested fungi reflecting a relatively longer genetic distance from other isolates of dermatophytes and yeasts. Candida krusei and Candida tropicalis were closely related showing 93.3% GS. C. albicans showed 90.9% similarity with other species of Candida. Epidermophyton floccosum was easily separated from all Trichophyton species showing 87.3% similarity. Unique bands were displayed by certain fungi and can be taken as a positive marker for isolate identification and discrimination. RFLP technique revealed differences in the number (1 to 5) and size (8 to 378 base pairs) of DNA fragments depending on the fungal isolate and restriction enzyme used. Within each fungal species, different isolates of dermatophytes and Candida from Egypt and Libya showed close relationship. Seven isozyme systems namely esterase, peroxidase, malate dehydrogenase, acid phosphatase, glutamate-oxalo-acetate transaminase, Urease and protease were studied to detect the gene expression and genetic variability among the different isolates of dermatophytes and Candida.

show abstract

“…These methods can be used to detect the pathogenic dermatophytes from the clinical samples with remarkable reliability. However, these methods are complicated and require pure DNA samples in large quantity, and thus are not suitable for rapid identification and examination of large groups of clinical samples [Faggi et al, 2001;Spesso et al, 2013].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection for Rabbit-Derived Dermatophytes Using Microsatellite-Primed Polymerase Chain Reaction

Miao

Song²,

Dai-Jun³

et al. 2013

Microb Physiol

View full text Add to dashboard Cite

A method exhibiting high sensitivity, specificity and rapidity to detect pathogenic dermatophytes was developed using microsatellite-primed polymerase chain reaction (PCR) in combination with a clustering method. The DNA fragments of Trichophyton mentagrophyton, Microsporum gypseum and Microsporum canis were amplified by using the primer (GACA)₄ to detect the DNA polymorphism fingerprints. Twenty-one clinical strains identified as T. mentagrophyton, M. gypseum or M. canis by morphological methods were distinguished according to the differences of standard stains' bands combined with NTSYS-pc2.10 software. The results showed that there were obvious and direct differences in the bands of the three pathogenic dermatophytes, and the similarity of isolated strains and standard strains were above 90%, in line with the results of morphological identification. The method is more accurate, rapid and simple, which is meaningful for the clinical diagnosis and epidemic research of the dermatophytes.

show abstract

Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes

Cited by 21 publications

References 28 publications

Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species

Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Rapid Detection for Rabbit-Derived Dermatophytes Using Microsatellite-Primed Polymerase Chain Reaction

Contact Info

Product

Resources

About