Microscale depletion of high abundance proteins in human biofluids using IgY14 immunoaffinity resin: analysis of human plasma and cerebrospinal fluid

Hyung, Seok Won; Piehowski, Paul; Moore, Ronald J.; Orton, Daniel J.; Schepmoes, Athena; Clauss, Therese RW; Chu, Rosalie K.; Fillmore, Thomas; Brewer, Heather M.; Liu, Tao; Zhao, Rui; Smith, Richard

doi:10.1007/s00216-014-8058-3

Cited by 11 publications

(10 citation statements)

References 53 publications

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“…Several powerful methods, such as CE [1,2], immobilized metal affinity chromatography (IMAC) [3,4] and immunoaffinity chromatography [5,6], have been applied to capture the high-abundance proteins from biological samples. However, the high cost, poor stability and difficult acuisition of antibodies [7] hinders the wide application of these approaches.…”

Section: Introductionmentioning

confidence: 99%

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Gao

Zhang

Hao

et al. 2015

J of Separation Science

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A novel magnetic core-shell polydopamine-cupric ion complex imprinted polymer was prepared in one-step through surface imprinting technology, which could specifically recognize bovine hemoglobin from the real blood samples. The polymerization conditions and adsorption performance of the resultant nanomaterials were investigated in detail. The results showed that the cupric ion played an important role in the recognition of template proteins. The saturating adsorption capacity of this kind of imprinted polymers was 2.23 times greater than those of imprinted polymers without cupric ion. The imprinting factor of the imprinted materials was as high as 4.23 for the template molecule. The selective separation bovine hemoglobin from the real blood sample is successfully applied. In addition, the prepared materials had excellent stability and no obvious deterioration after five adsorption-regeneration cycles. Easy preparation, rapid separation, high binding capacity and satisfactory selectivity for the template protein make this polymer attractive in the separation of high-abundance proteins.

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Section: Introductionmentioning

confidence: 99%

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Gao

Zhang

Hao

et al. 2015

J of Separation Science

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“…29 Unfortunately, Tf is one of the proteins depleted using these commercial kits, making it necessary to seek alternative ways of albumin depletion. We accomplished this using blue dye resin (BDR), which has a high affinity to albumin.…”

Section: Resultsmentioning

confidence: 99%

Assessing the iron delivery efficacy of transferrin in clinical samples by native electrospray ionization mass spectrometry

Pawlowski

Kellicker

Bobst

et al. 2016

Analyst

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Serum transferrin is a key player in iron homeostasis, and its ability to deliver iron to cells via the endosomal pathway critically depends on the presence of carbonate that binds this protein synergistically with ferric ion. Oxalate is another ubiquitous anionic species that can act as a synergistic anion, and in fact its interaction with transferrin is notably stronger compared to carbonate, preventing the protein from releasing the metal in the endosomal environment. While this raises concerns that high oxalate levels in plasma may interfere with iron delivery to tissues, concentration of free oxalate in blood appears to be a poor predictor of impeded availability of iron, as previous studies showed that it cannot displace carbonate from ferro-transferrin on a physiologically relevant time scale under the conditions mimicing plasma. In this work we present a new method that allows different forms of ferro-transferrin (carbonate-vs oxalate-bound) to be distinguished from each other by removing this protein from plasma without altering the composition of the protein/metal/synergistic anion complexes, and determining their accurate masses using native electrospray ionization mass spectrometry (ESI MS). The new method has been validated using a mixture of recombinant proteins, followed by its application to the analysis of clinical samples of human plasma, demonstrating that native ESI MS can be used in clinical analysis.

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“…Manuscript to be reviewed triethylammonium bicarbonate (TEAB) at pH 8.5. Concentrated fractions were stored at -80°C (18).…”

Section: Immunoaffinity Depletionmentioning

confidence: 99%

Peer Review #2 of "Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis (v0.1)"

2019

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Background. Leishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed. Objective: The aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response. Methods. Four patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry (LC-MS). Results. Twelve proteins showed differential expression in the comparative LCelectrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were upregulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response. Conclusion. We identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in cutaneous leishmaniasis.

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Microscale depletion of high abundance proteins in human biofluids using IgY14 immunoaffinity resin: analysis of human plasma and cerebrospinal fluid

Cited by 11 publications

References 53 publications

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Assessing the iron delivery efficacy of transferrin in clinical samples by native electrospray ionization mass spectrometry

Peer Review #2 of "Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis (v0.1)"

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