One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Self Cite

Glycoproteins are crucial in massive physiological events and clinical application. It is necessary to prepare new materials to isolate the specific glycoprotein. New and simple core-shell molecularly imprinted polymers were prepared by surface imprinting. The polymers are synthesized with magnetic nanoparticles as the core, water-soluble dendritic polyethyleneimine as the monomer and the ovalbumin as the template. The prepared imprinted polymers showed thin imprinted shell, biocompatibility and superparamagnetic properties. The resultant materials exhibited fast kinetics, high adsorption capacity, perfect selectivity and reusability. More important, they can absorb the template glycoprotein from the neutral solution and successfully be applied to recognize the ovalbumin from egg white, which means that they can provide an alternate method to isolate glycoprotein from bodily fluids.

“…The diameter of Fe 3 O 4 exhibiting in Fig. A and B is about 270 nm, which matches well with our previous study . After the imprinting process, Fig.…”

Section: Resultssupporting

confidence: 91%

“…Fe 3 O 4 nanoparticles (denoted as Fe 3 O 4 ) were prepared as our previous work . FeCl 3 ·6H 2 O (1.013 g), NaOAc (2.7 g), and PEG (0.075 g) were dissolved in EG (15 mL) in a Teflon‐lined stainless‐steel autoclave.…”

Section: Methodsmentioning

confidence: 99%

Preparation of magnetic glycoprotein‐imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white

Zhang

Tang

Hao

et al. 2016

Self Cite

“…In recent years, molecular imprinting solid‐phase extraction has been extensively used to efficiently separate trace compounds from complex matrixes . For example, molecular imprinting solid‐phase extraction was used to extract thiabendazole from citrus , folates from tomatoes , bovine hemoglobin from the bovine blood , and GA from natural resources . Hu et al.…”

Section: Introductionmentioning

confidence: 99%

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Zhang

Yue

et al. 2017

With the combined surface imprinting technique and immobilized template strategy, molecularly imprinted magnetic nanoparticles were successfully prepared and coupled with high-performance liquid chromatography to selectively separate and determine gallic acid from the pomegranate rind. On the surface of carboxyl-functionalized magnetic nanospheres, thin imprinting shells were formed using dopamine as monomer and crosslinker. The characteristics, polymerization conditions, and adsorption performances of the resultant nanomaterials were investigated in detail. In addition of good crystallinity, satisfactory magnetism, and uniform morphology of the obtained polymers, they had rapid binding kinetics, high adsorption capacity, and favorable reusability. In the mixed solution of four hydroxybenzoic acids, the prepared nanomaterials have an excellent selectivity to gallic acid with an imprinting factor of as high as 17.5. Therefore, the polymers have great potentials in specific extraction and enrichment of gallic acid from the complex natural resources.

“…Recently, the authors of some excellent review papers discussed different formats of MIPs . Also, numerous papers revealed the application of MIPs for the determination of bioanalytes such as bioamines . The MIPs were also successfully applied in fabrication of sensors and drug delivery devices .…”

Section: Introductionmentioning

confidence: 99%

Development of a validated strategy for the determination of tryptamine in human cerebrospinal fluid in the presence of competitors using molecularly imprinted polymers

Luliński

Bamburowicz-Klimkowska

Dana

et al. 2017

This study presents a validated strategy for the determination of tryptamine in the presence of its competitors, which involves the molecularly imprinted solid-phase extraction combined with high-performance liquid chromatography coupled with fluorimetric detection. Tryptamine-imprinted microscale sorbent was produced from 4-vinylbenzoic acid and ethylene glycol dimethacrylate in methanol by precipitation polymerization, and its imprinting factor was equal to 15.4 in static experiments or 18.6 in dynamic binding experiments. The method for tryptamine determination in the presence of serotonin and l-tryptophan was validated using a complex matrix of bovine serum albumin yielding the recoveries of tryptamine that ranged between 98.7 and 107.0%. Very low limits of detection and limits of quantification for tryptamine (19.9 and 60.3 nmol/L, respectively) allow the quantification of tryptamine in human cerebrospinal fluid in the presence of tryptophan and serotonin.