Preparation of magnetic glycoprotein‐imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white

Zhang, Lili; Tang, Yuhai; Hao, Yi; He, Gaiyan; Zhang, Bianbian; Gao, Ruixia; Zhang, Min

doi:10.1002/jssc.201600112

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…The adsorption capacities of different adsorbents for OVA are summarized in Table . Since its 3D porous architecture provides more adsorption sites for OVA, the theoretical adsorption capacity of the NiO‐rGOA composite has an obvious improvement compared with other adsorbents reported previously .…”

Section: Resultsmentioning

confidence: 88%

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In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

Chen

Zheng

Zhang

et al. 2017

J of Separation Science

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A three-dimensional reduced graphene oxide aerogel with embedded nickel oxide nanoparticles was prepared by a one-step self-assembly reaction in a short time. The nanoparticles could be captured into the interior of reduced graphene oxide network during the formation of the three-dimensional architecture. The composite exhibited porosity, good biocompatibility, and abundant metal affinity binding sites. The aerogel was used to isolate ovalbumin selectively from egg white, and favorable adsorption was achieved at pH 3. An adsorption efficiency of 90.6% was obtained by using 1 mg of the composite for adsorbing 70 μg/mL of ovalbumin in 1.0 mL of sample solution, and afterwards a recovery of 90.7% was achieved by using an eluent of 1.0 mL Britton-Robinson buffer solution at pH 5. After the adsorption/desorption, ovalbumin showed no change in the conformation. The adsorption behavior of ovalbumin on the reduced graphene oxide composite well fitted to the Langmuir adsorption model, and a corresponding theoretical maximum adsorption capacity was 1695.2 mg/g. A sodium dodecyl sulfate polyacrylamide gel electrophoresis assay demonstrated that the aerogel could selectively isolate ovalbumin from chicken egg white.

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Section: Resultsmentioning

confidence: 88%

“…Besides, molecularly imprinted polymers show good selective to specific substance. The magnetic glycoprotein‐imprinted nanoparticles was prepared by Zhang et al., which exhibited the specific recognition of OVA from egg white .…”

Section: Introductionmentioning

confidence: 99%

In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

Chen

Zheng

Zhang

et al. 2017

J of Separation Science

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“…Some new strategies need to be tried. Molecular imprinting technology (MIT) is a technique with good recognition capacity and high selectivity, which has been employed in the analysis and separation field successfully. − The cavities in the imprinted polymer can match the targets in shape, size, and functional groups. In our previous research, we have proved that MIT is able to improve the purity of bovine serum albumin (BSA) on mix-mode chromatography through introducing double recognition.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Adsorption Selectivity of Ion-Exchange Chromatography through the Double-Recognition Mode

Shi

Zhang

et al. 2019

J. Chem. Eng. Data

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Ion-exchange chromatography (IEC) was combined with molecular imprinting technology (MIT) to prepare two kinds of doublerecognition resins (DEAE@BSA and DEAE@bIgG) using bovine serum albumin (BSA) and bovine immunoglobulin G (bIgG) as the imprinting template proteins. New resins consist of ion-exchange interaction and spatial recognition and were characterized by Fourier transform infrared, scanning electron microscopy−energy-dispersive X-ray spectrometry, thermogravimetric analysis, and Brunauer−Emmett−Teller techniques. The static and dynamic adsorption behaviors of proteins on resins were investigated. In addition, the competitive adsorption between BSA and bIgG was also studied. It was found that the adsorbed protein density (Q c ) of the target protein remained the same after imprinting, but the competitive protein dropped obviously. The selectivity factors (α) of DEAE@BSA and DEAE@ bIgG could reach 3.56 and 2.56, respectively. The results of adsorption kinetics and frontal adsorption experiments further showed that the dynamic adsorption capacity of target proteins was improved in batch and column modes. Taken together, this study demonstrated that the selectivity of IEC could be improved with the double-recognition mode.

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“…Separation of a solid from an aqueous solution represents a huge issue in engineering aspect. Compared with traditional MIP, magnetic molecularly imprinted polymers (MMIPs) have been used in many complicated matrices such as egg white sample and serum sample , because the MMIP has unique magnetic properties that enable them to be easily separated from the matrix under an external magnetic field after adsorption and recognition.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a magnetic molecularly imprinted polymer by atom-transfer radical polymerization for the extraction of parabens from fruit juices

You

Piao²,

Chen

2016

J. Sep. Science

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A silica-based surface magnetic molecularly imprinted polymer for the selective recognition of parabens was prepared using a facile and general method that combined atom-transfer radical polymerization with surface imprinting technique. The prepared magnetic molecularly imprinted polymer was characterized by transmission electron microscopy, Fourier transform infrared spectrometry and physical property measurement. The isothermal adsorption experiment and kinetics adsorption experiment investigated the adsorption property of magnetic molecularly imprinted polymer to template molecule. The four parabens including methylparaben, ethylparaben, propylparaben, and butylparaben were used to assess the rebinding selectivity. An extraction method, which used magnetic molecularly imprinted polymer as adsorbents coupled with high-performance liquid chromatography for the determination of the four parabens in fruit juice samples was developed. Under the optimal conditions, the limits of detections of the four parabens were 0.028, 0.026, 0.021, and 0.026 mg/L, respectively. The precision expressed as relative standard deviation ranging from 2.6 to 8.9% was obtained. In all three fortified levels, recoveries of parabens were in the range of 72.5-89.4%. The proposed method has been applied to different fruit juice samples including orange juice, grape juice, apple juice and peach juice, and satisfactory results were obtained.

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Preparation of magnetic glycoprotein‐imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white

Cited by 11 publications

References 33 publications

In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

In situ self‐assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high‐efficiency separation of ovalbumin

Improvement of Adsorption Selectivity of Ion-Exchange Chromatography through the Double-Recognition Mode

Preparation of a magnetic molecularly imprinted polymer by atom-transfer radical polymerization for the extraction of parabens from fruit juices

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