Microscopic and Voltammetric Characterization of Bioanalytical Platforms Based on Lactate Oxidase

Parra, Ana Matus de la; Casero, Elena; Vázquez, Luís; Jin, Jiye; Pariente, F.; Lorenzo, Encarnación

doi:10.1021/la060184g

Cited by 24 publications

(25 citation statements)

References 28 publications

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“…[20][21][22][23][24][25][26][27][28][29] But, an important problem in GC application is the electrochemical-response reproducibility related to the electrode pretreatment. 30,31 Diverse investigations showed that the electrodes obtained by different voltammetric methods had different behaviors to the adsorption of organic molecules.…”

mentioning

confidence: 99%

Correlation Between the Distribution of Oxide Functional Groups and Electrocatalytic Activity of Glassy Carbon Surface

Vettorazzi

Sereno

Katoh

et al. 2008

J. Electrochem. Soc.

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Oxidative electrochemical pretreatment on glassy carbon ͑GC͒ electrodes in aqueous media produces changes in the relative densities of oxidized species present on the material surface. These changes vary with both applied potential and time procedure. The GC surface oxidation treatment not only increases the oxygen-containing group coverage but also causes lattice damage, producing a highly porous dielectric film. In this work we report the gradual relationship between the kinetics of the heterogeneous charge transfer after different pretreatment procedures and the surface distribution of oxidized species in GC measured through electron spectroscopy for chemical analysis. The lack of faradaic current observed when the electrode surface is oxidized under the constant-potential regime is proposed to be due to changes in the lattice structure, observed by scanning electron microscopy. This fact is important in systems where carbon materials are used as electrodes under the continuous-oxidation regime, such as high-performance liquid chromatography electrochemical detectors, batteries, capacitors, and for the generation of polymers by potentiostatic oxidation.

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mentioning

confidence: 99%

Correlation Between the Distribution of Oxide Functional Groups and Electrocatalytic Activity of Glassy Carbon Surface

Vettorazzi

Sereno

Katoh

et al. 2008

J. Electrochem. Soc.

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“…Tapping mode has been developed in an aqueous environment, thus allowing characterization of the enzyme electrodes in their working state. Indeed “the biological relevance of dry samples might be questioned” since “imaging under ambient conditions implies the application of higher forces on the protein, which could damage or distort the imaged structures” . However AFM can rarely provide information about the catalytic activity of immobilized enzymes, although we present here an example where time resolved AFM has enabled to visualize motions of protein domains during the catalytic cycle (see part 8).…”

Section: Microscopy and Nanoscopymentioning

confidence: 99%

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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Redox enzymes, which catalyze electron transfer reactions in living organisms, can be used as selective and sensitive bioreceptors in biosensors, or as efficient catalysts in biofuel cells. In these bioelectrochemical devices, the enzymes are immobilized at a conductive surface, the electrode, with which they must be able to exchange electrons. Different physicochemical methods have been coupled to electrochemistry to characterize the enzyme‐modified electrochemical interface. In this Review, we summarize most efforts performed to investigate the enzymatic electrodes at the micro‐ and even nanoscale, thanks to microscopy techniques. Contrary to electrochemistry, which gives only a global information about all processes occurring at the electrode surface, microscopy offers a spatial resolution. Several techniques have been implemented; mostly scanning probe microscopies like atomic force microscopy, scanning tunneling microscopy, and scanning electrochemical microscopy, but also scanning electron microscopy and fluorescence microscopy. These studies demonstrate that various information can be obtained thanks to microscopy at different scales. Electrode imaging has been performed to confirm the presence of enzymes, to quantify and localize the biomolecules, but also to evaluate the morphology of immobilized enzymes, their possible conformation changes upon turnover, and their orientation at the electrode surface. Local redox activity has also been imaged and kinetics has been resolved.

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“…First of all, the LOx were immobilized by direct absorption on glassy carbon electrodes and highly ordered pyrolytic graphite and the LOx layers has been characterized using microscopic techniques. The lactate could be amperometrically determined [200]. In a new report, it was presented a lactate biosensor based on the immobilization of lactate oxidase (LOx) using two different strategies including direct adsorption and covalent binding onto gold surfaces.…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

State-of-the-Art of (Bio)Chemical Sensor Developments in Analytical Spanish Groups

Plata

Contento

Ríos

2010

Sensors

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(Bio)chemical sensors are one of the most exciting fields in analytical chemistry today. The development of these analytical devices simplifies and miniaturizes the whole analytical process. Although the initial expectation of the massive incorporation of sensors in routine analytical work has been truncated to some extent, in many other cases analytical methods based on sensor technology have solved important analytical problems. Many research groups are working in this field world-wide, reporting interesting results so far. Modestly, Spanish researchers have contributed to these recent developments. In this review, we summarize the more representative achievements carried out for these groups. They cover a wide variety of sensors, including optical, electrochemical, piezoelectric or electro-mechanical devices, used for laboratory or field analyses. The capabilities to be used in different applied areas are also critically discussed.

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Microscopic and Voltammetric Characterization of Bioanalytical Platforms Based on Lactate Oxidase

Cited by 24 publications

References 28 publications

Correlation Between the Distribution of Oxide Functional Groups and Electrocatalytic Activity of Glassy Carbon Surface

Correlation Between the Distribution of Oxide Functional Groups and Electrocatalytic Activity of Glassy Carbon Surface

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

State-of-the-Art of (Bio)Chemical Sensor Developments in Analytical Spanish Groups

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