Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients

Oyama, Kotaro; Mizuno, Akari; Shintani, Seine A.; Itoh, Hideki; Serizawa, Takahiro; Fukuda, Norio; Suzuki, Madoka; Ishiwata, Shin’ichi

doi:10.1016/j.bbrc.2011.12.015

Cited by 45 publications

(77 citation statements)

References 24 publications

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“…This increase in pacing threshold may provide some insight into the mechanism of infrared stimulation in the heart. For example, it has been shown that infrared stimulation can cause cardiomyocytes to contract by affecting actin-myosin interactions [43], which CytoD disrupts [44]. It is possible that normal optical pacing of cardiomyocytes works through a combination of contraction and alteration of membrane capacitance [35], and the abolition of motion therefore increases the optical pacing threshold.…”

Section: Discussionmentioning

confidence: 99%

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Wang

et al. 2014

Biomed. Opt. Express

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Cardiac electrophysiology plays a critical role in the development and function of the heart. Studies of early embryonic electrical activity have lacked a viable point stimulation technique to pace in vitro samples. Here, optical pacing by high-precision infrared stimulation is used to pace excised embryonic hearts, allowing electrophysiological parameters to be quantified during pacing at varying rates with optical mapping. Combined optical pacing and optical mapping enables electrophysiological studies in embryos under more physiological conditions and at varying heart rates, allowing detection of abnormal conduction and comparisons between normal and pathological electrical activity during development in various models. 440-447 (1978). 12. K. Kamino, "Optical approaches to ontogeny of electrical activity and related functional organization during early heart development," Physiol. Rev. 71(1), 53-91 (1991 Macrae, and D. S. Rosenbaum, "The zebrafish as a novel animal model to study the molecular mechanisms of mechano-electrical feedback in the heart," Prog. Biophys. Mol. Biol. 110(2-3), 154-165 (2012). 18. M. Bressan, G. Liu, and T. Mikawa, "Early mesodermal cues assign avian cardiac pacemaker fate potential in a tertiary heart field," Science 340 (

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Section: Discussionmentioning

confidence: 99%

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Wang

et al. 2014

Biomed. Opt. Express

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“…IR pulses could also produce contraction of cardiomyocytes in the Ca 2þ -free media and without noticeable Ca 2þ transients. 27,28 These results suggest that internal Ca 2þ modulatory mechanisms might dominate over Ca 2þ influx during IR stimulation.…”

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confidence: 83%

Ryanodine and IP₃ receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation

et al. 2017

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Abstract. Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from IR exposure could activate temperature-or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. Studies using fluorescent intensity-based calcium ion (Ca 2þ ) sensitive dyes show changes in Ca 2þ levels after various IR stimulation parameters, which suggests that Ca 2þ may originate from the external solution. However, activation of intracellular signaling pathways has also been demonstrated, indicating a more complex mechanism of increasing intracellular Ca 2þ concentration. We quantified the Ca 2þ mobilization in terms of influx from the external solution and efflux from intracellular organelles using Fura-2 and a high-speed ratiometric imaging system that rapidly alternates the dye excitation wavelengths. Using nonexcitable Chinese hamster ovarian (CHO-hM 1 ) cells and neuroblastomaglioma (NG108) cells, we demonstrate that intracellular IP 3 receptors play an important role in the IR-induced Ca 2þ , with the Ca 2þ response augmented by ryanodine receptors in excitable cells.

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“…The microscope with local heating systems was previously described (11). Confocal fluorescence images were captured with a confocal unit (CSU-X1, Yokogawa Electric, Tokyo, Japan), an electron multiplying charge-coupled device camera (iXon-Ultra, Andor Technologies plc, Belfast, Northern Ireland), and an objective lens (PlanApo N 60Â/1.45 Oil, Olympus, Tokyo, Japan) attached to the inverted microscope (IX-70, Olympus).…”

Section: Optical Setupmentioning

confidence: 99%

“…This thermosensing function of ion channels is used to remotely control neural activity with radiofrequency magnetic field local heating (6,7). Local heating itself can also induce local gene expression (8), membrane depolarization (9,10), and muscle contraction (11)(12)(13). These local heating techniques have a strong potential for the spatial control of cell functions.…”

Section: Introductionmentioning

confidence: 98%

Directional Bleb Formation in Spherical Cells under Temperature Gradient

Oyama

Arai

Isaka

et al. 2015

Biophysical Journal

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Living cells sense absolute temperature and temporal changes in temperature using biological thermosensors such as ion channels. Here, we reveal, to our knowledge, a novel mechanism of sensing spatial temperature gradients within single cells. Spherical mitotic cells form directional membrane extensions (polar blebs) under sharp temperature gradients (≥∼0.065°C μm(-1); 1.3°C temperature difference within a cell), which are created by local heating with a focused 1455-nm laser beam under an optical microscope. On the other hand, multiple nondirectional blebs are formed under gradual temperature gradients or uniform heating. During heating, the distribution of actomyosin complexes becomes inhomogeneous due to a break in the symmetry of its contractile force, highlighting the role of the actomyosin complex as a sensor of local temperature gradients.

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Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients

Cited by 45 publications

References 24 publications

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Ryanodine and IP₃ receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation

Directional Bleb Formation in Spherical Cells under Temperature Gradient

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Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients

Cited by 45 publications

References 24 publications

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Optical stimulation enables paced electrophysiological studies in embryonic hearts

Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation

Directional Bleb Formation in Spherical Cells under Temperature Gradient

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Product

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About

Ryanodine and IP₃ receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation