“…Biological cell counting is an important method to evaluate the progress of cell proliferation and the diagnosis of various diseases. Automated cell counting using software based morphological analysis has been employed in several biological and medical applications including: red blood cell (RBC) counting, white blood cells (WBC) counting, embryonic stem cell counting and tumour detection in mammography (Abbas, 2015;Chourasiya et al, 2014;Joshi et al, 2013;Hsu, 2012;Ghosh et al, 2016, Sandhaus, 2015Yi et al, 2013, Shirazi et al, 2016Tomari et al, 2014;Faustino et al, 2009).…”