Microscopic Instrumentation and Analysis of Laser-Tissue Interaction in a Skin Flap Model

Gourgouliatos, Z. F.; Welch, Ashley J.; Diller, Kenneth R.

doi:10.1115/1.2894888

Cited by 18 publications

(19 citation statements)

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“…After a bolus injection, the ICG plasma concentration decay is usually described as a biexponential decay. Ott et al [14] ) is in accordance with the data of literature about ICG halflife in plasma (3-5 minutes) [2,17]. Two different ICG formulations were used.…”

Section: Indocyanine Green Dye (Icg)mentioning

confidence: 91%

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Selective laser photocoagulation of blood vessels in a hamster skin flap model using a specific ICG formulation

et al. 1997

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Section: Indocyanine Green Dye (Icg)mentioning

confidence: 91%

“…This model is based on one developed previously by Papenfuss et al [13] and slightly modified by Gourgouliatos [14]. Each plate has a 18 mm central viewing aperture, and they are attached together surgically to provide support for the flap.…”

Section: Hamster Skin-flap Window Modelmentioning

confidence: 99%

Selective laser photocoagulation of blood vessels in a hamster skin flap model using a specific ICG formulation

et al. 1997

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“…The dorsal area of each hamster was shaved and epilated to remove hair roots. The dorsal skin flap window preparation (13,14) was implanted using the following procedure. The skin flap was pulled away from the body at the dorsal midline and sutured to a vertical C-Clamp.…”

Section: Methodsmentioning

confidence: 99%

Morphological Changes in Blood Vessels Produced by Hyperosmotic Agents and Measured by Optical Coherence Tomography¶

Vargas¹,

Readinger²,

Dozier³

et al. 2003

Photochem Photobiol

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Optical tissue clearing by hyperosmotic chemical agents significantly increases light depth penetration in skin and may improve light-based therapeutics such as laser treatment of cutaneous vascular lesions. A feasibility study was conducted to evaluate the potential role of optical clearing by glycerol in laser treatment of cutaneous vessels. Optical imaging was performed to investigate the morphological effects of glycerol on blood vessels of skin. Blood vessels were imaged using Doppler optical coherence tomography in in vivo hamster skin treated with glycerol. Images were obtained from the subdermal side to assess morphological changes in the blood vessels caused by glycerol and from the epidermal side to assess enhanced Doppler imaging of blood vessels. Application of glycerol to the subdermis resulted in venule stasis and for prolonged treatment times, arteriole stasis. In cases where flow remained in arterioles, an improved Doppler signal was detected from blood vessels when imaging transepidermally compared with the native condition. Intensity images indicated changes in blood optical properties and improved contrast of skin cross sections after glycerol application. The observed optical and morphological effects were reversed upon hydration of the skin with phosphate-buffered saline. The combination of increased depth of light penetration and the temporary slowing or cessation of flow in blood vessels could mean improved laser treatment of vessels.

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“…The window consists of two identical and symmetric aluminium plates. This model is based on one developed previously by Papenfuss et al [16] and slightly modified by Gourgouliatos et al [17]. Each plate has a 18 mm central viewing aperture and are attached together surgically to provide support for the flap.…”

Section: Materials and Methods Hamster Skin-flap Window Modelmentioning

confidence: 99%

Thermal damage assessment of blood vessels in a hamster skin flap model by fluorescence measurement of a liposome-dye system

et al. 1997

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Background and Objectives The present study was undertaken to evaluate the feasibility of thermal damage assessment of blood vessels by using laser‐induced release of liposome‐encapsulated dye. Study Design/Materials and Methods Experiments were performed in a hamster skin flap model. Laser irradiation was achieved with a 300 μm fiber connected to a 805 nm diode laser (power=0.8W, spot diameter=1.3 mm and pulse exposure time lasting from 1 to 6 s) after potentiation using a specific indocyanine green (ICG) formulation (water and oil emulsion). Liposomes‐encapsulated carboxyfluorescein were prepared by the sonication procedure. Carboxyfluorescein (5,6‐CF) was loaded at high concentration (100 mM) in order to quench its fluorescence. The measurements were performed after i.v. injection of DSPC liposomes (1.5 ml) and lasted 40 min. Fluorescence emission was measured with an ultra high sensitivity intensified camera. Results Three different shapes of fluorescent spots were identified depending on target (blood vessel or skin) and energy deposition in tissue: (i) intravascular fluorescence, (ii) transient low fluorescence circular spot, and (iii) persistent high intense fluorescence spot. These images are correlated with histological data. Conclusion Real‐time fluorescence imaging seems to be a good tool to estimate in a non‐invasive manner the thermal damage induced by a diode laser combined with ICG potentiation. Lasers Surg. Med. 20:131–141, 1997. © 1997 Wiley‐Liss, Inc.

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Microscopic Instrumentation and Analysis of Laser-Tissue Interaction in a Skin Flap Model

Cited by 18 publications

References 0 publications

Selective laser photocoagulation of blood vessels in a hamster skin flap model using a specific ICG formulation

Selective laser photocoagulation of blood vessels in a hamster skin flap model using a specific ICG formulation

Morphological Changes in Blood Vessels Produced by Hyperosmotic Agents and Measured by Optical Coherence Tomography¶

Thermal damage assessment of blood vessels in a hamster skin flap model by fluorescence measurement of a liposome-dye system

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