Microscopic investigations of palynological features of convolvulaceous species from arid zone of Pakistan

Abstract

The pollen morphology of some species of glamorous family Convolvulaceae from arid zones of Pakistan has been evaluated. The pollen grains were acetolyzed, measured, described qualitatively, and illustrated using Light microscopy and scanning electron microscopy (SEM). The examined species have differences in shape, size, polarity and exine ornamentation, confirming the eurypalynous character. The pollen types varies from psilate to echinate and colpi to tricolporate, tetracolporate and pantocolporate. Exine ornamentation was exposed as perforate, reticulate and echinate. Spine morphology and exine sculpture are vital for the distinction of species. Pollen fertility shows that selected plants species are well-known in the Arid Zone. A taxonomic key is prepared to use these variations in the identification of species. Statistical analysis by using the Software XLSTAT exhibited that some morphological features is the main characters in identification of the taxa. These studied taxa were separated from each other using cluster Analysis. Our results constructed on MVSP ver. 3.22 software analyses that show morphological explanation; thus, the results highlight the importance of pollen morphology in the identification and characterization of species of the family convolvulacae in arid zone.

Foliar epidermal anatomy of Lamiaceae with special emphasis on their trichomes diversity using scanning electron microscopy

“…Additionally, SEM are a valuable research instrument that increasing our fundamental knowledge about previously unrecorded structural components of plants with high magnifications and resolution (Newton, ; Stant, ). Some previous studies (Ashfaq et al, ; Bahadur et al, ; Mannethody & Purayidathkandy, ; Moon, Hong, Smets, & Huysmans, ; Mousavi, Jafari, & Najafi, ; Salmaki et al, ; Ullah et al, ) also showed the importance of SEM in the identification, discrimination and phylogenetic relationship of other groups of plants.…”

Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy

“…Exine often reticulate, microreticulate scabrate, and psilate are taxonomically significant features examined under SEM to identify monocot taxa at microlevel. Similarly, Ashfaq et al (), Bahadur et al (), Butt et al (), Kosenko (), Sufyan et al (), Ullah, Zafar, Ahmad, Dilbar, et al () investigated pollen of various taxa, using LM and SEM techniques and reported that the pollen morphological characteristics were of important characters for the taxonomic identifications of species and genera in different plant families. The LM and SEM study show variation between the species with special emphasis on exine ornamentation, which has a significant role to support the taxonomy of problematic and closely related taxa (Carrijo et al, ; Lopes, Andreata, Cartaxo‐Pinto, Trovó, & Gonçalves‐Esteves, ; Nurit‐Silva & De Fátima Agra, ).…”

Microscopic investigation of pollen morphology of Brassicaceae from Central Punjab‐Pakistan

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