Microscopic structure of gelatin coacervates

Mohanty, Biswaranjan; Bohidar, H. B.

doi:10.1016/j.ijbiomac.2005.03.012

Cited by 67 publications

(26 citation statements)

References 42 publications

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“…5) shows coacervates of dense matter with a large heterogeneity without definite geometric structures in some parts, and fibril structures in other parts. The coexisting coacervates and fibril structures are very similar to those of an acid-treated pig gelatin (Uricanu et al, 2003), and an alkaline-treated gelatin (Mohanty & Bohidar, 2005). However, the pretreatment information for these latter two gelatins has not been reported although the results suggest that the two gelatins may have been pretreated with acid before extraction.…”

Section: Effects Of Pretreatments On Nanostructure Changes Of the Extmentioning

confidence: 84%

“…For example, Atomic force microscopy (AFM) has been one of the most powerful instruments for the characterization of nanostructures of food-related materials Yang, Lai, An, & Li, 2006;Yang, Wang, Lai et al, 2007). It has been applied to some biologically purified gelatins and hybrid gels (Benmouna & Johannsmann, 2004;Haugstad & Gladfelter, 1993, 1994Lin et al, 2002;Mackie, Gunning, Ridout, & Morris, 1998;Mohanty & Bohidar, 2005;Radmacher, Fritz, & Hansma, 1995;Saxena, Sachin, Bohidar, & Verma, 2005;Uricanu et al, 2003;Yang, Wang, Regenstein, & Rouse, 2007;Yao, Liu, Lin, & Qiu, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Effects of alkaline and acid pretreatment on the physical properties and nanostructures of the gelatin from channel catfish skins

et al. 2008

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Section: Effects Of Pretreatments On Nanostructure Changes Of the Extmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Effects of alkaline and acid pretreatment on the physical properties and nanostructures of the gelatin from channel catfish skins

et al. 2008

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“…Γ should satisfy the Young equation: γ ag = Γ + γ ap cosθ, where γ ag and γ ap are the interfacial tensions between the air/ gelatin and air/PEG phases, respectively, and the θ is the contact angle between the air bubble and the gelatin-gel surface. We measured θ by changing the temperature and obtained a value of Γ by assuming the previously reported values of γ ag ∼ 70 mN/m (26,27) (Fig. 3B, Right).…”

Section: Significancementioning

confidence: 99%

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

Yanagisawa

Nigorikawa

Sakaue

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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We report the spontaneous patterning of polymer microgels by confining a polymer blend within microspheres. A poly(ethylene glycol) (PEG) and gelatin solution was confined inside water-in-oil (W/O) microdroplets coated with a layer of zwitterionic lipids: dioleoylphosphatidylethanolamine (PE) and dioleoylphosphatidylcholine (PC). The droplet confinement affected the kinetics of the phase separation, wetting, and gelation after a temperature quench, which determined the final microgel pattern. The gelatin-rich phase completely wetted to the PE membrane and formed a hollow microcapsule as a stable state in the PE droplets. Gelation during phase separation varied the relation between the droplet size and thickness of the capsule wall. In the case of the PC droplets, phase separation was completed only for the smaller droplets, wherein the microgel partially wetted the PC membrane and had a hemisphere shape. In addition, the temperature decrease below the gelation point increased the interfacial tension between the PEG/ gelatin phases and triggered a dewetting transition. Interestingly, the accompanying shape deformation to minimize the interfacial area was only observed for the smaller PC droplets. The critical size decreased as the gelatin concentration increased, indicating the role of the gel elasticity as an inhibitor of the deformation. Furthermore, variously patterned microgels with spherically asymmetric shapes, such as discs and stars, were produced as kinetically trapped states by regulating the incubation time, polymer composition, and droplet size. These findings demonstrate a way to regulate the complex shapes of microgels using the interplay among phase separation, wetting, and gelation of confined polymer blends in microdroplets. microgels | aqueous two-phase systems | sol-gel phase separation | hydrogels | emulsions T he regulation of the 3D shapes of biopolymer gels at the mesoscale has numerous applications in the biomedical, cosmetic, and food materials industries, among others (1). Recently, top-down and bottom-up approaches have been reported to control the mesoscopic patterns of polymer gels. For example, photolithography and two-photon polymerization allow the regulation of gel patterns at the mesoscale (2-4). The advanced design of the molecules enables polymerization with a self-assembly and produces nonspherical microgels: spherical particles with a cavity, capsules, and cubic particles (5-7). However, these methods require highly specialized equipment and are generally difficult to adapt for biopolymer gels.Dynamical coupling between phase separation and sol-gel transition in polymer blends has also been investigated for the spontaneous formation of spherical microgels and a porous gel (8, 9). Ma et al. (10) and Choi et al. (11) confined aqueous two-phase systems (ATPSs) in microdroplets and fabricated microgels by selective polymerization. In such a confined space, phase separation accompanies wetting of a polymer to the substrate (12-15). Although the selective polymerization of phase-se...

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“…Gelatin gels are different in that they are prone to create many hydrogen bonds with increased weight-to-volume ratios provided the solvent is appropriate. Once above 30°C, the gelatin molecules have induced conformational changes to form a triple helix, creating a network of gelatin fibers that are stabilized by hydrogen bonding [89,90]. Furthermore, both collagen and gelatin have employed shared crosslinking strategies that employ chemical, enzymatic, or physical means.…”

Section: Collagen and Gelatinmentioning

confidence: 99%

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

Balikov

Lee

Boire

et al. 2015

Biosystems &Amp; Biorobotics

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Because of their potential to regenerate tissues and organs, stem cells garner extensive interest worldwide for treating a wide range of degenerative diseases. In numerous efforts, a variety of stem cell formats have been considered for therapeutic purposes to combat such pathologies, one being vascular-related diseases. In particular, the prevalence of peripheral artery disease (PAD) has steadily increased with the growth of the aging population in many first-world countries. Considering this disturbing trend as well as the obesity epidemic and the ballooning population growth in third-world nations, the burden of PAD is expected to increase worldwide to alarming levels in the coming decades. The advent of stem cell treatments could stymie this burden by alleviating the complications and improving upon the less-than-satisfactory outcomes from current standard-of-care surgical/pharmacological interventions for PAD. This chapter reviews the relevant, cutting-edge clinical and animal model research efforts in the field and explores the remaining questions as they pertain to convergence technologies that may provide the potential for stem cells to reverse PAD-induced tissue damage. Harnessing and translating this potential to create more viable and efficacious PAD treatments should be of paramount global and public health concern.

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Microscopic structure of gelatin coacervates

Cited by 67 publications

References 42 publications

Effects of alkaline and acid pretreatment on the physical properties and nanostructures of the gelatin from channel catfish skins

Effects of alkaline and acid pretreatment on the physical properties and nanostructures of the gelatin from channel catfish skins

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

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