Microscopical Lesions and Antigen Distribution in Bovine Fetal Tissues and Placentae Following Experimental Infection with Bovine Herpesvirus-1 during Pregnancy

Rodger, S. M.; Murray, J.; Underwood, Charlie J.; Buxton, D.

doi:10.1016/j.jcpa.2007.04.022

Cited by 19 publications

(21 citation statements)

References 8 publications

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“…17 The single case where BoHV-1 DNA was only detected in the placenta supports the hypothesis that placental damage may precede spread to the fetus, 24,26,29 and occasionally that viral insult is enough to cause abortion, in contrast to the hypothesis that placental lesions are a result of spread from fetal infection. 16 The presence of BoHV-1 within the endothelium of the blood vessels in the placenta has been previously observed 17,26 and, in an experimental challenge, virus was detected by the fluorescent antibody test despite the absence of placental lesions. 8 Abortion occurring in absence of a fetal infection has also been noted in cases of EHV-1 equine abortion, suggested to be due to failure of the vascular supply caused by EHV-1 infection.…”

Section: Discussionmentioning

confidence: 70%

Bovine herpesvirus 1abortion

et al. 2012

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Abstract. While Bovine herpesvirus 1 (BoHV-1) has been known as a cause of bovine abortion for nearly 50 years, information is limited on the current prevalence of BoHV-1 abortion in the United Kingdom, or about the mode of virus dissemination to cause infection of the fetus. The present study aimed to investigate these issues by surveying the prevalence of BoHV-1 in abortion cases in the United Kingdom, and comparing diagnostic methods to determine which are most efficient in BoHV-1-induced abortion. Where BoHV-1 DNA was detected, viral load was compared in fetal tissues, using realtime polymerase chain reaction (PCR), supported by histopathology and immunohistochemistry (IHC) to investigate virus dissemination in bovine abortions. A total of 400 U.K. bovine abortion cases were studied; PCR detected BoHV-1 nucleic acids in 10 cases, suggestive histopathological lesions were observed in 8, and positive IHC staining was observed in 9. In routine diagnosis, BoHV-1 was identified in 2 of these cases, highlighting the utility of using molecular diagnostic tests such as real-time PCR to achieve high sensitivity in potentially autolyzed tissues. The study of different fetal samples showed the highest viral load in the liver, along with severe multifocal necrotic hepatitis, suggesting either a clear tropism of the virus for this organ or that it is the first location to be reached in the fetus. Presence of viral antigen in endothelial cells of the placenta, brain, or heart suggest a hematogenous spread of virus from placenta to the liver, through the umbilical vein, and then to the rest of the organs via fetal blood vessels.

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Section: Discussionmentioning

confidence: 70%

Bovine herpesvirus 1abortion

et al. 2012

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“…Outros autores descrevem necrose linfoide com depleção secundá-ria (Enright et al 1984, Rodger et al 2007) e necrose e infiltrado inflamatório mononuclear periportal no fígado, além de focos de necrose pulmonar e hemorragias na região medular dos rins (Rodger et al 2007) em casos de aborto por Herpesvírus bovino tipo 1. Fig.10.…”

Section: Discussionunclassified

“…Essas diferenças podem estar relacionadas ao uso de diferentes cepas do vírus. Rodger et al (2007) descrevem marcação em hepatócitos e em células de baço e linfonodos, nestes tanto na região medular como na cortical. Essa marcação nos tecidos linfoides foi semelhante ao encontrado neste estudo.…”

Section: Discussionunclassified

Caracterização morfológica e imuno-histoquímica de lesões em casos de aborto bovino bacteriano e viral no sul de Minas Gerais

et al. 2014

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Lavras, from 1999Lavras, from to 2013. From 60 fetuses studied, 30 (50%) had microscopic lesions. From these, eight had lesions consistent with bacterial agents and three had lesions suggestive of viral agents. In the bacterial abortions, one fetus presented lesions compatible with leptospirosis, characterized by jaundice, cholestasis, lymphoplasmacytic intersticial nephritis, and tubular nephrosis. Seven fetuses had purulent pneumonia or bronchopneumonia and one of them had also fibrinous pleuritis and peritonitis; two of them presented positive immunostaining for Brucella abortus. One of the three fetuses with lesions of viral infection revealed positive imunostaining for bovine herpesvirus. The results showed that abortions of bacterial and viral origin occur in the Region of this study and prophylactic measures should be adopted on the farms. This study also demonstrates that immunohistochemistry associated with histopathology is a useful and viable tool for the diagnosis, especially when microbiological and/or serological tests are not available.

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“…In a study 27 conducted to determine the distribution of BHV1 antigen in experimentally infected pregnant heifers, BHV1 antigen was detected in the mesenchyme of fetal villi and endothelial cells. In a study 27 conducted to determine the distribution of BHV1 antigen in experimentally infected pregnant heifers, BHV1 antigen was detected in the mesenchyme of fetal villi and endothelial cells.…”

Section: Discussionmentioning

confidence: 99%