Microscopy and Image Analysis

McNamara, George; Difilippantonio, Michael J.; Ried, Thomas; Bieber, Frederick R.

doi:10.1002/cphg.42

Cited by 23 publications

(13 citation statements)

References 280 publications

(282 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Objectives also naturally present with color artifacts, that is, chromatic aberrations or color “fringes.” Objectives that have been color corrected via the combination of unique glasses, each holding different color refraction properties, are followed by the suffix “apochromat.” Historically, apochromatic lenses would correct for three to four spectral lines. [ 71 ] Some modern WSI models include apochromatic lenses correcting for up to 14 spectral lines, that is, fully color corrected, for example, Carl Zeiss apochromatic.…”

Section: Mage Q Uality and R Esolutionmentioning

confidence: 99%

Contemporary Whole Slide Imaging Devices and Their Applications within the Modern Pathology Department: A Selected Hardware Review

Patel

Balis

Cheng

et al. 2021

Journal of Pathology Informatics

View full text Add to dashboard Cite

Section: Mage Q Uality and R Esolutionmentioning

confidence: 99%

Contemporary Whole Slide Imaging Devices and Their Applications within the Modern Pathology Department: A Selected Hardware Review

Patel

Balis

Cheng

et al. 2021

Journal of Pathology Informatics

View full text Add to dashboard Cite

“…Chromosome analysis may be done through the microscope or by the use of digital images with karyotyping analysis software on desktop computers. A number of digital‐image‐capture devices are available ( UNIT , McNamara et al., ). These devices, which can substantially speed the counting and analysis of chromosomes and provide a digital “photograph” of chromosomes, are currently in use in most cytogenetic laboratories.…”

Section: Tissue Sourcesmentioning

confidence: 99%

Overview of Clinical Cytogenetics

2016

View full text Add to dashboard Cite

Chromosome analysis is one of the first approaches to genetic testing and remains a key component of genetic analysis of constitutional and somatic genetic disorders. Numerical or unbalanced structural chromosome abnormalities usually lead to multiple congenital anomalies. Sometimes these are compatible with live birth, usually resulting in severe cognitive and physical handicaps; other times they result in miscarriage or stillbirth. Chromosome rearrangements also occur as somatic changes in malignancies. Identification of constitutional chromosomal anomalies (anomalies present in most or all cells of the body and/or the germline) can provide important information for genetic counseling. In this unit, we introduce chromosomal microarray analysis (CMA), which is a relatively recent addition to cytogenetic technologies, and has become the recommended first-tier testing method for patients with developmental delay, intellectual disability, autism, and/or multiple congenital anomalies. We also discuss non-invasive prenatal testing/screening (NIPTS), which uses circulating cell-free fetal DNA (cfDNA) from maternal plasma to rapidly screen for autosomal and sex-chromosome aneuploidies. Cytogenetic analysis of tumors is helpful in diagnosis and in monitoring the effects of treatment. The protocols in this chapter cover the clinical study of chromosomes in nonmalignant tissues.

show abstract

“…Digital image analysis method (Calvaruso et al, 2009; Chen, Li, Dong, & Pei, 2018; Madabhushi & Lee, 2016; McNamara, Difilippantonio, Ried, & Bieber, 2017) can be used to perform quantitative evaluations of reticular fiber fragments. Fragment counting under a microscope can be carried out by a specialist by continually looking at the microscope lens or by automatic cell counting methods (Belsare & Mushrif, 2012; Gurcan et al, 2009; Madabhushi & Lee, 2016;Yegin et al, 2015; Yegin, Yegin, & Ozdogan, 2016).…”

Section: Introductionmentioning

confidence: 99%

A digital image analysis study on the disintegration kinetics of reticular fibers in the ethylene glycol‐induced rat liver tissue

Saraydın

İnan

2020

Microscopy Res & Technique

View full text Add to dashboard Cite

Ethylene glycol (EG), the raw material of polyethylenterephthalate, which is the most consumed plastic in the world, has low toxicity, but its metabolites are toxic. EG metabolites can cause acidosis, fibrosis, and eventually cirrhosis in the liver. This study aimed to investigate the effect of EG on rat liver and to determine the quantitative values of the disintegration of reticular fibers (RF) in the liver with the dose duration and to investigate the changes by digital image analysis (DIA). For this purpose, Wistar albino rats were divided into control, and five different daily experimental groups. The control group received saline, and the experimental groups received EG. At the end of experiments, liver tissues of all euthanized rats were removed, and sections were taken, and RF was shown by silver staining. It was observed that the RF fragments in the experimental groups were less than the control group. DIA of RF fragments was then performed with Olympus cellSensDimension 1.15 software and number, area, and ROI% values of the fragments were determined. Statistical analysis revealed that there was a significant difference between control and all experimental groups. RF fragments showed first-order disintegration kinetics, mean disintegration rate constant, and half-time values were 0.1 day −1 and 7 days, respectively. Consequently, the digital image analysis approach can be a useful tool for the biologist, pathologist, fibrosis-cirrhosis specialist, and computer scientist to understand the effects of toxic chemicals in the liver and analyze reticular fiber disintegration.

show abstract

Microscopy and Image Analysis

Cited by 23 publications

References 280 publications

Contemporary Whole Slide Imaging Devices and Their Applications within the Modern Pathology Department: A Selected Hardware Review

Contemporary Whole Slide Imaging Devices and Their Applications within the Modern Pathology Department: A Selected Hardware Review

Overview of Clinical Cytogenetics

A digital image analysis study on the disintegration kinetics of reticular fibers in the ethylene glycol‐induced rat liver tissue

Contact Info

Product

Resources

About