Microscopy, Culture, and Quantitative Real-Time PCR Examination Confirm Internalization of Mycobacteria in Plants

Kaevska, Marija; Lvončík, Samuel; Slaná, Iva; Kulich, Pavel; Králík, Petr

doi:10.1128/aem.00496-14

Cited by 14 publications

(4 citation statements)

References 26 publications

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“…paratuberculosis and M. avium ssp. hominissuis ) in plant tissues (i.e., roots, stem, fruit, and leaf) of other plants was confirmed in pinto bean ( Phaseolus vulgaris ) [ 85 ], grass [ 86 ], lettuce ( Lactuca sativa ), tomato ( Solanum lycopersicum ), and radish ( Raphanus raphanistrum ssp. sativus ) [ 78 ].…”

Section: Mycobacteria and Sapronosesmentioning

confidence: 99%

Nontuberculous Mycobacteria as Sapronoses: A Review

et al. 2022

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Mycobacteria are a unique group of microorganisms. They are characterised by exceptional adaptability and durability. They are capable of colonisation and survival even in very unfavourable conditions. In addition to the well-known obligate human pathogens, Mycobacterium tuberculosis and M. leprae, more than 200 other species have been described. Most of them form a natural part of the microflora of the external environment and thrive in aquatic and soil environments especially. For many of the mycobacterial species associated with human disease, their natural source has not yet been identified. From an ecological point of view, mycobacteria are saprophytes, and their application in human and animal diseases is opportunistic. Most cases of human disease from saprophytic mycobacteria occur in immunocompromised individuals. This adaptability and resilience to environmental pressures makes treatment of mycobacterial diseases (most often sapronoses and less often zoonoses) and permanent eradication of mycobacteria from the environment very difficult. Saprophytic mycobacterial diseases (sapronoses) are chronic and recurrent due to the fact of repeated endogenous or exogenous re-exposure. Therefore, knowledge regarding their occurrence in soil and dust would aid in the prevention of saprophytic mycobacterioses. In conjunction, their presence and ecological significance in the environment can be revealed.

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Section: Mycobacteria and Sapronosesmentioning

confidence: 99%

Nontuberculous Mycobacteria as Sapronoses: A Review

et al. 2022

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“…M. terramassiliense , M. rhizamassiliense , and M. numidiamassiliense were first described in tomato plant roots [ 210 ] and M. sarraceniae and M. helvum were first isolated in the pitcher plant in Minnesota sphagnum peat bogs [ 211 ]. M. avium was detected in the stems and leaves of different plant species after internalization into plant tissue through intact, as well as damaged, root systems, which can imply the role of plants in the spread and transmission of mycobacteria to other organisms in the environment [ 212 ]. Moreover, several plant-based foods have been investigated and found to be contaminated with NTM, namely those that are close to, or beneath, the soil surface [ 213 ].…”

Section: Mycobacteria Ecological Nichesmentioning

confidence: 99%

Non-Tuberculous Mycobacteria: Molecular and Physiological Bases of Virulence and Adaptation to Ecological Niches

et al. 2020

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Non-tuberculous mycobacteria (NTM) are paradigmatic colonizers of the total environment, circulating at the interfaces of the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. Their striking adaptive ecology on the interconnection of multiple spheres results from the combination of several biological features related to their exclusive hydrophobic and lipid-rich impermeable cell wall, transcriptional regulation signatures, biofilm phenotype, and symbiosis with protozoa. This unique blend of traits is reviewed in this work, with highlights to the prodigious plasticity and persistence hallmarks of NTM in a wide diversity of environments, from extreme natural milieus to microniches in the human body. Knowledge on the taxonomy, evolution, and functional diversity of NTM is updated, as well as the molecular and physiological bases for environmental adaptation, tolerance to xenobiotics, and infection biology in the human and non-human host. The complex interplay between individual, species-specific and ecological niche traits contributing to NTM resilience across ecosystems are also explored. This work hinges current understandings of NTM, approaching their biology and heterogeneity from several angles and reinforcing the complexity of these microorganisms often associated with a multiplicity of diseases, including pulmonary, soft-tissue, or milliary. In addition to emphasizing the cornerstones of knowledge involving these bacteria, we identify research gaps that need to be addressed, stressing out the need for decision-makers to recognize NTM infection as a public health issue that has to be tackled, especially when considering an increasingly susceptible elderly and immunocompromised population in developed countries, as well as in low- or middle-income countries, where NTM infections are still highly misdiagnosed and neglected.

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“…We are here reporting on natural infection. Accordingly, the number of colony-forming units (CFUs) estimated in the roots was 60 CFU/g which is five logs lesser than the 10 6 CFU/g detected in roots in the experimentally model 11 .…”

Section: Discussionmentioning

confidence: 73%

“…On the other hand, it was shown experimentally that M . avium can internalize in the roots of tomato plants and spread in its different tissues, namely stem, leaves and fruits 11 . We are here reporting on natural infection.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium terramassiliense, Mycobacterium rhizamassiliense and Mycobacterium numidiamassiliense sp. nov., three new Mycobacterium simiae complex species cultured from plant roots

Bouam

Armstrong

Levasseur

et al. 2018

Sci Rep

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Three slowly growing mycobacteria named strain AB308, strain AB215 and strain AB57 were isolated from the tomato plant roots. The 16S rRNA and rpoB gene sequence analyses suggested that each strain was representative of one hitherto unidentified slowly-growing Mycobacterium species of the Mycobacterium simiae complex. Genome sequencing indicated that each strain contained one chromosome of 6.015–6.029 Mbp. A total of 1,197, 1,239 and 1,175 proteins were found to be associated with virulence and 107, 76 and 82 proteins were associated with toxin/antitoxin systems for strains AB308, AB215 and AB57, respectively. The three genomes encode for secondary metabolites, with 38, 33 and 46 genes found to be associated with polyketide synthases/non-ribosomal peptide synthases and nine, seven and ten genes encoding for bacteriocins, respectively. The genome of strain AB308 encodes for one questionable prophage and three incomplete prophages, while only incomplete prophages were predicted in AB215 and AB57 genomes. Genetic and genomic data indicate that strains AB308, AB215 and AB57 are each representative of a new Mycobacterium species that we respectively named Mycobacterium terramassiliense, Mycobacterium numidiamassiliense and Mycobacterium rhizamassiliense.

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Microscopy, Culture, and Quantitative Real-Time PCR Examination Confirm Internalization of Mycobacteria in Plants

Cited by 14 publications

References 26 publications

Nontuberculous Mycobacteria as Sapronoses: A Review

Nontuberculous Mycobacteria as Sapronoses: A Review

Non-Tuberculous Mycobacteria: Molecular and Physiological Bases of Virulence and Adaptation to Ecological Niches

Mycobacterium terramassiliense, Mycobacterium rhizamassiliense and Mycobacterium numidiamassiliense sp. nov., three new Mycobacterium simiae complex species cultured from plant roots

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