Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography

Paturzo, Melania; Fińizio, Andrea; Memmolo, Pasquale; Puglisi, Roberto; Balduzzi, Donatella; Galli, Andrea; Ferraro, Pietro

doi:10.1039/c2lc40114b

Cited by 83 publications

(42 citation statements)

References 23 publications

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“…This enhancement may be attributed to the shift of Doppler-frequency experienced by the photons scattered from the flowing particles. 59,60 The decrease in flow velocity may reduce the light scattered from RBCs. Eventually, the reduction of scattering light may lead to the decrease of LI during the initial state.…”

Section: Microfluidic-based Methodsmentioning

confidence: 99%

Microfluidic-based speckle analysis for sensitive measurement of erythrocyte aggregation: A comparison of four methods for detection of elevated erythrocyte aggregation in diabetic rat blood

Yeom

Lee

2015

Biomicrofluidics

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Biochemical alterations in the plasma and red blood cell (RBC) membrane of diabetic blood lead to excessive erythrocyte aggregation (EA). EA would significantly impede the blood flow and increase the vascular flow resistance contributing to peripheral vascular diseases. In this study, a simple microfluidic-based method is proposed to achieve sensitive detection of hyperaggregation. When a blood sample is delivered into the device, images of blood flows are obtained with a short exposure time for a relatively long measuring time. A micro-particle image velocimetry technique was employed to monitor variation of the flow rate of blood as a function of time. Given that EA formation in the channel creates clear speckle patterns, the EA extent can be estimated by calculating a speckle area (ASpeckle) through a normalized autocovariance function. The hematocrit effect is assessed by comparing optical images transmitted through blood samples. EA variations caused by dextran treatment are quantitatively evaluated using characteristic time (λSpeckle) obtained by fitting the variations of ASpeckle. Other indices including number of RBCs in an aggregate (NRBC), characteristic time of erythrocyte sedimentation rate (λESR), and aggregation index estimated from ultrasound signals (AIEcho) are determined under different EA conditions using conventional techniques. The four different methods are applied to diabetic blood samples to compare their indices under hyperaggregation conditions. It is found that the proposed method can detect variation of EA reasonably, compared with conventional measurement techniques. These experimental demonstrations support the notion that the proposed method is capable of effectively monitoring the biophysical properties of diabetic blood.

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Section: Microfluidic-based Methodsmentioning

confidence: 99%

Microfluidic-based speckle analysis for sensitive measurement of erythrocyte aggregation: A comparison of four methods for detection of elevated erythrocyte aggregation in diabetic rat blood

Yeom

Lee

2015

Biomicrofluidics

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“…In recent years, digital holography (DH) [1] has become an high-throughput imaging instrument able to furnish a diagnostic tool for microfluidic at lab-on-chip scale [2][3][4]. Among others features, DH allows accurate retrieving of the 3D positions of multiple particles performing three-dimensional (3D) tracking of the sample in the entire field of view.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional holographic tracking approach based on full-field complex wavefront matching

Memmolo

Miccio

Merola

et al. 2014

Frontiers in Optics 2014

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“…[5][6][7][8][9][10][11][12][13][14] Since the information in the phase image ( , ) is a quantitative measure of the morphology of the tissue biopsy, diagnosis based on QPI provides the potential to eliminate inter and intra observer variation and lends itself to automation.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of breast cancer biopsies using quantitative phase imaging

et al. 2015

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The standard practice in the histopathology of breast cancers is to examine a hematoxylin and eosin (H&E) stained tissue biopsy under a microscope. The pathologist looks at certain morphological features, visible under the stain, to diagnose whether a tumor is benign or malignant. This determination is made based on qualitative inspection making it subject to investigator bias. Furthermore, since this method requires a microscopic examination by the pathologist it suffers from low throughput. A quantitative, label-free and high throughput method for detection of these morphological features from images of tissue biopsies is, hence, highly desirable as it would assist the pathologist in making a quicker and more accurate diagnosis of cancers. We present here preliminary results showing the potential of using quantitative phase imaging for breast cancer screening and help with differential diagnosis. We generated optical path length maps of unstained breast tissue biopsies using Spatial Light Interference Microscopy (SLIM). As a first step towards diagnosis based on quantitative phase imaging, we carried out a qualitative evaluation of the imaging resolution and contrast of our label-free phase images. These images were shown to two pathologists who marked the tumors present in tissue as either benign or malignant. This diagnosis was then compared against the diagnosis of the two pathologists on H&E stained tissue images and the number of agreements were counted. In our experiment, the agreement between SLIM and H&E based diagnosis was measured to be 88%. Our preliminary results demonstrate the potential and promise of SLIM for a push in the future towards quantitative, label-free and high throughput diagnosis.

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Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography

Cited by 83 publications

References 23 publications

Microfluidic-based speckle analysis for sensitive measurement of erythrocyte aggregation: A comparison of four methods for detection of elevated erythrocyte aggregation in diabetic rat blood

Microfluidic-based speckle analysis for sensitive measurement of erythrocyte aggregation: A comparison of four methods for detection of elevated erythrocyte aggregation in diabetic rat blood

Three-dimensional holographic tracking approach based on full-field complex wavefront matching

Diagnosis of breast cancer biopsies using quantitative phase imaging

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