Microsphere-assisted microscopy

Darafsheh, Arash

doi:10.1063/5.0068263

Cited by 50 publications

(45 citation statements)

References 154 publications

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“…At present time, the true resolution limit of microlens-assisted microscopy is under debate. In most works, structures with subdiffraction features are used to estimate the resolution, which can lead to its overestimation [18,33,34].…”

Section: Photonic Nanojetsmentioning

confidence: 99%

“…The size of the smallest visible element dropped to ~50 nm. Since 2011, numerous studies have emerged concerning the acquisition of ultra-high resolution images with microsphere-assisted conventional optical microscopes [18]. Unlike other ultra-high resolution methods, this technology allows visualization of objects under white light illumination in real time without labels.…”

Section: Introductionmentioning

confidence: 99%

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Microlens‐assisted microscopy for biology and medicine

Trukhova

Pavlova

Синицына

et al. 2022

Journal of Biophotonics

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The addition of dielectric transparent microlens in the optical scheme is an effective and at the same time simple and inexpensive way to increase the resolution of a light microscope. For these purposes, spherical and cylindrical microlenses with a diameter of 1–100 μm are usually used. The microlens focuses the light into a narrow beam called a photonic nanojet. An enlarged virtual image is formed, which is captured by the objective of the light microscope. In addition to microscopy, the microlenses are successfully applied to amplify optical signals, increase the trapping force of optical tweezers and are used in microsurgery. This review considers the design and principle of microlens‐assisted microscopes. Taking into account the advantages of the super‐resolution optical methods for research in life science, the examples of the use of the microlenses in biomedical practice are discussed in detail.

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Section: Photonic Nanojetsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microlens‐assisted microscopy for biology and medicine

Trukhova

Pavlova

Синицына

et al. 2022

Journal of Biophotonics

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“…This value of the resolution is perfectly consistent with theoretical evaluations available in the literature. [ 50 ] Lastly, from this result, it is possible to calculate the lower limit of the spatial resolution enhancement due to the presence of the microsphere. Indeed, considering an initial spatial resolution of about 700 nm (diffraction limit), the ability to distinguish two different QDs separated by 300 nm results in a 2.3× spatial resolution enhancement.…”

Section: Spatial Resolutionmentioning

confidence: 99%

Photonic Jets and Single‐Photon Emitters

Ristori

Felici

Pettinari

et al. 2022

Advanced Photonics Research

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Photonic jets (PJs) obtained by illuminating a dielectric microsphere have recently shown to provide an efficient and cost‐effective means of laser‐writing and localizing single‐photon emitters with sub‐diffraction precision. The fabrication technique relies on the photoinduced formation of GaAsN quantum dots (QDs) that are self‐aligned to the microsphere, which in turn enhances the collection efficiency of their emission. Similarly, the angular magnification introduced by a microsphere placed on top of two close emitters allows to detect and resolve their separation below the diffraction limit by analyzing their angular emission pattern in momentum space. Along with a brief review of the two methods, a systematic numerical study on the formation and properties of PJs to streamline the optimization of the fabrication process is presented.

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“…An optical resolution as small as 50 nm has been demonstrated with this method 33 . The easy-to-implement nature of this method makes it an attractive and affordable way to boost the resolution of a conventional microscope 34 . However, the superior resolution of this method is achieved at a significantly reduced field of view, which is essentially reduced to the size of the microsphere.…”

Section: Introductionmentioning

confidence: 99%

An acoustofluidic scanning nanoscope using enhanced image stacking and processing

et al. 2022

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Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire field of view (200 × 200 μm) by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs. Moreover, the image quality is also greatly improved by applying an alpha-blending imaging technique, which is critical for accurately depicting and identifying nanoscale objects or processes. This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.

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Microsphere-assisted microscopy

Cited by 50 publications

References 154 publications

Microlens‐assisted microscopy for biology and medicine

Microlens‐assisted microscopy for biology and medicine

Photonic Jets and Single‐Photon Emitters

An acoustofluidic scanning nanoscope using enhanced image stacking and processing

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