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Figure S1) magnified images of Blu-ray disc surface are taken by different microscope objective and SEM; (Figures S2) analysis of super-resolution effects in different materials and sizes of microparticles; (Figures S3) simulation results of a 2 μm PS bead along two orthogonal directions with FDTD method; (Figure S4) Ge nanoparticles imaging through a PS-20 microsphere; (Figure S5) schematic explanation of reflection and transmission light illuminations; (Figure S6) comparison of optical resolutions of 800 nm chrome grating patterns of letter "K"; (Table S1) percentage of the FOV imaged versus the number of imaging frames and processing time (PDF) Movie of microparticles movement by an acoustic energy distribution. (AVI) Movie of microparticles movement and tracking. (AVI)

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Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire field of view (200 × 200 μm) by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs. Moreover, the image quality is also greatly improved by applying an alpha-blending imaging technique, which is critical for accurately depicting and identifying nanoscale objects or processes. This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.