Microsphere‐Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1‐MMP

Ku, Minhee; Kim, Hyun Joon; Yau, Su Yee; Yoon, Nara; Kim, Nam Hee; Yook, Jong In; Kim, Dae Eun; Yang, Jaemoon

doi:10.1002/smll.201803000

Cited by 6 publications

(4 citation statements)

References 52 publications

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“…Accordingly, HT-1080 cells were incubated on PNHC for 6 h, and we analyzed the images of PNHC before and after treatment ( Figure 7 a,b). We confirmed that several cells were attached well and were largely elongated on the PNHC surface, indicating that the cells were under active metabolism (for example, glycolysis) [ 38 ]. The B/G values were extracted from around the attached cells in the image and compared with those of the bare PNHC.…”

Section: Resultsmentioning

confidence: 53%

Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Lee

Kim

et al. 2022

Nanomaterials

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Hydrogels containing colorimetric nanoparticles have been used for ion sensing, glucose detection, and microbial metabolite analyses. In particular, the rapid chemical reaction owing to both the hydrogel form of water retention and the sensitive color change of nanoparticles enables the rapid detection of target substances. Despite this advantage, the poor dispersibility of nanoparticles and the mechanical strength of nanoparticle–hydrogel complexes have limited their application. In this study, we demonstrate a milliliter agarose gel containing homogeneously synthesized polyaniline nanoparticles (PAni-NPs), referred to as PAni-NP–hydrogel complexes (PNHCs). To fabricate the optimal PNHC, we tested various pH solvents based on distilled water and phosphate-buffered saline and studied the colorimetric response of the PNHC with thickness. The colorimetric response of the prepared PNHC to the changes in the pH of the solution demonstrated excellent linearity, suggesting the possibility of using PNHC as a pH sensor. In addition, it was verified that the PNHC could detect minute pH changes caused by the cancer cell metabolites without cytotoxicity. Furthermore, the PNHC can be stably maintained outside water for approximately 12 h without deformation, indicating that it can be used as a disposable patch-type wearable biosensing platform.

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Section: Resultsmentioning

confidence: 53%

Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Lee

Kim

et al. 2022

Nanomaterials

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“…We measured the nanomechanical strength of the HAP/Col hydrogels using the 10.8 μm polystyrene-bead tip. Because the size of the AFM probe tip is much larger than the pore of each hydrogel, it is assumed that the diverse pore size of the hydrogel hardly influenced the estimation of the nanomechanical properties of the HAP/Col hydrogels …”

Section: Resultsmentioning

confidence: 99%

Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles

Jung

Lee

et al. 2021

ACS Omega

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Hydroxyapatite nanoparticle-complexed collagen (HAP/Col) hydrogels have been widely used in biomedical applications as a scaffold for controlled drug release (DR). The time-variant mechanical properties (Young’s modulus, E ) of HAP/Col hydrogels are highly relevant to the precise and efficient control of DR. However, the correlation between the DR and the E of hydrogels remains unclear because of the lack of a nondestructive and continuous measuring system. To reveal the correlations, herein, we investigate the time-variant behavior of E for HAP/Col hydrogels during 28 days using the atomic force microscopy (AFM) nanoindentation technique. The initial E of hydrogels was controlled from 200 to 9000 Pa by the addition of HAPs. Subsequently, we analyzed the relationship between the DR of the hydrogels and the changes in their mechanical properties (Δ E ) during hydrogel degradation. Interestingly, the higher the initial E value of HAP/Col hydrogels is, the higher is the rate of hydrogel degradation over time. However, the DR of hydrogels with higher initial E appeared to be significantly delayed by up to 40% at a maximum. The results indicate that adding an appropriate amount of HAPs into hydrogels plays a crucial role in determining the initial E and their degradation rate, which can contribute to the properties that prolong DR. Our findings may provide insights into designing hydrogels for biomedical applications such as bone regeneration and drug-delivery systems.

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“…21 Eventually, the investigation of the change of cytoskeletal network is critical to anticipate the survival of cancer patients. Extending our previous study on the cytoskeletal network for cancer cells, 22 thus, we have focused on the change of cytoskeletal network from cancer cells treated with phenolics-loaded nanocarriers.…”

Section: T H Imentioning

confidence: 99%

Antioxidative Impact of Phenolics-Loaded Nanocarriers on Cytoskeletal Network Remodeling of Invasive Cancer Cells

Jung

Jeong

et al. 2023

ACS Appl. Mater. Interfaces

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Natural phenolic compounds have antioxidant properties owing to their free radical-scavenging capability. The combined effect of a mixture of phenolic compounds has been studied; however, the detailed investigation for finding a correlation between single phenolic molecules and antioxidant activity has not been explored. Herein, we revealed that the number of phenolic hydroxyl groups in phenolics played a central role in their antioxidant capacity. Based on the finding, tannic acid showed the most effective antioxidant potential, e.g., 76% in tannic acid versus 22% in vitamin C as a standard antioxidant component. Because cancer progression is closely related to oxidative processes at the cellular level, we further applied the surface treatment of tannic acid drug-delivery nanocarriers. Tannic acid-loaded nanocarriers reduced reactive oxygen species of cancer cells as much as 41% of vehicle treatment and remodeled cytoskeletal network. By a gelatin degradation study, TA-loaded nanocarrier-treated cells induced 44.6% reduction of degraded area than vehicletreated cells, implying a potential of blocking invasiveness of cancer cells.

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Microsphere‐Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1‐MMP

Cited by 6 publications

References 52 publications

Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles

Antioxidative Impact of Phenolics-Loaded Nanocarriers on Cytoskeletal Network Remodeling of Invasive Cancer Cells

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